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pcs |
| Victor ID |
995 |
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Gene Name |
pcs |
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Sequence Strain (Species/Organism) |
Brucella suis 1330 |
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NCBI Gene ID |
1165011
|
|
NCBI Protein GI |
23500316
|
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Locus Tag |
BRA0572 |
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Genbank Accession |
AE014292 |
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Protein Accession |
NP_699756 |
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Other Database IDs |
UniProtKB-ID: Q8FW88_BRUSU
UniRef100: UniRef100_Q8FW88
UniRef90: UniRef90_A5VUQ6
UniRef50: UniRef50_Q98MN3
UniParc: UPI00000DD9B4
EMBL: AE014292
EMBL-CDS: AAN33761.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0572
NMPDR: fig|204722.1.peg.2665
TIGR: BRA0572
HOGENOM: HBG675964
OMA: FLHPFRV
ProtClustDB: CLSK863002
BioCyc: BSUI204722:BR_A0572-MONOMER
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Taxonomy ID |
204722
|
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Chromosome No |
II |
|
Gene Starting Position |
550525 |
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Gene Ending Position |
551325 |
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Gene Strand (Orientation) |
- |
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Protein Name |
phosphatidylcholine synthase |
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Protein pI |
10.31 |
|
Protein Weight |
26888.95 |
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Protein Length |
266 |
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Protein Note |
similar to GP:6739568; identified by sequence similarity; putative |
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DNA Sequence |
>gi|56968493:550525-551325 Brucella suis 1330 chromosome chromosome II, complete sequence
ATCATGGTGCTTCTCCGCTCTTCTGTTGCTTTTCAACAAGTTTTCGGGCCTTGGCAAGTGCAGCTTCACG
ACCAAGGCTTGGGAAAAGCTGCATGATCGCGCCGATGAAATAGATGTAAAGGCCGGTTACGGAAATGCCG
ATACGGACCCAGAGCGGCGCATCCAGCATGTAATAAAGGGCAATCACGCCAAACGCGCACCAGAGCAGGA
AAATCGTGAGGTTGAGCGGGCGCAGCCGCACCACCCGCACCGGATGCAGGAAGTTGATCGGCAGGAAGGA
AAGGATCGCCGAAGCCACGACCGTTCCGAAGGCCACCCATTCGCCGGGCCTGACGATGAAAAGCATGAAA
ACCACCATGTTCCAGACCACGGGGAAACCCTTGAAGAAGTTCTCCTTGGTCTTCATGCCGGTATCAGCAT
AATAGATCGCGCTTGAAACCACGATGATCGCGCCGGAAATGAAGGACAGGTTCGTCCCCATGAACCCGCT
TTGATAGAGCGCGAAAGCGGGTATCAGCACATAGGTAACATAGTCGATAATACTGTCGAGAAGCTCGCCA
GACCAGTTCGGCAGGACATATTTGACTTTAAGCTTACGCGCGATGGGGCCGTCTATGCCATCCACGAACA
GCGCGAGGCCCAGCCACCACCACATGGCGGTATAGCGCCCGTCGCTTGCCGCCACCACGGAAAGGAAGGC
CAGAAACGAGCCGGAAGCCGTCAGAAGGTGAACGGAAAATGCCTTGGCCTGCGGGGCCGTGACTTTCTTG
GCCTTCAGTTTTCCGGTCAGTTTGGTTTTCA
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Protein Sequence |
>gi|23500316|ref|NP_699756.1| phosphatidylcholine synthase [Brucella suis 1330]
MKTKLTGKLKAKKVTAPQAKAFSVHLLTASGSFLAFLSVVAASDGRYTAMWWWLGLALFVDGIDGPIARK
LKVKYVLPNWSGELLDSIIDYVTYVLIPAFALYQSGFMGTNLSFISGAIIVVSSAIYYADTGMKTKENFF
KGFPVVWNMVVFMLFIVRPGEWVAFGTVVASAILSFLPINFLHPVRVVRLRPLNLTIFLLWCAFGVIALY
YMLDAPLWVRIGISVTGLYIYFIGAIMQLFPSLGREAALAKARKLVEKQQKSGEAP
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
MUTATION: The role of Phosphatidylcholine (PC) in Brucella abortus was examined by generating mutants in pcs (BApcs) and pmtA (BApmtA), which encode key enzymes of the two bacterial PC biosynthetic routes, the choline and methyl-transferase pathways. In rich medium, BApcs and the double mutant BApcspmtA but not BApmtA displayed reduced growth, increased phosphatidylethanolamine and no PC, showing that Pcs is essential for PC synthesis under these conditions. In minimal medium, the parental strain, BApcs and BApmtA showed reduced but significant amounts of PC suggesting that PmtA may also be functional Probing with phage Tb, antibiotics, polycations and serum demonstrated that all mutants had altered envelopes. In macrophages, BApcs and BApcspmtA showed reduced ability to evade fusion with lysosomes and establish a replication niche. In mice, BApcs showed attenuation only at early times after infection, BApmtA at later stages and BApcspmtA throughout. The results suggest that Pcs and PmtA have complementary roles in vivo related to nutrient availability and that PC and the membrane properties that depend on this typical eukaryotic phospholipid are essential for Brucella virulence (Conde-Alvarez et al., 2006). |
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COG
|
COG1183I, under I: Lipid transport and metabolism |
| References |
Conde-Alvarez et al., 2006: Conde-Alvarez R, Grilló MJ, Salcedo SP, de Miguel MJ, Fugier E, Gorvel JP, Moriyón I, Iriarte M. Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus. Cellular microbiology. 2006; 8(8); 1322-1335. [PubMed: 16882035].
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