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hemH from Brucella suis 1330

Victor ID 971
Gene Name hemH from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1164513
NCBI Protein GI 23499843
Locus Tag BRA0076
Protein Accession NP_699283.1
Other Database IDs UniProtKB-ID: HEMH_BRUSU
UniRef100: UniRef100_C0RK28
UniRef90: UniRef90_B2SCP3
UniRef50: UniRef50_A9MDJ3
UniParc: UPI0000058301
EMBL: AE014292
EMBL-CDS: AAN33288.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0076
TIGR: BRA0076
HOGENOM: HBG697135
OMA: TIEEIGM
ProtClustDB: PRK00035
BioCyc: BSUI204722:BR_A0076-MONOMER
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 74754
Gene Ending Position 75812
Gene Strand (Orientation) -
Protein Name ferrochelatase
DNA Sequence 
>gi|56968493:74754-75812 Brucella suis 1330 chromosome II, complete genome
TCTAAACCCATCCCAGCAATTCGCGGCGCACCAGCGTCTCAATGACCTTCATGCCCTCTTCGCTATCGTT
GAGGCAGGGAATATGGCTGAAGTTTTCGCCGCCGTTTTCAAGAAACTCTTCCGCTGCCTCATTGCCGATT
TCGTCCACCGTTTCCAGGCAATCGGCCACGAAACCAGGATTGAGCACCGCCACCGATTTGACGCCGTTTT
TGGCCAGTTCCTTCACGGTTTCGTCGGTATAGGGCTGGAGCCATTCTTCCGGGCCGAAGCGTGACTGGAA
GGTGGCGCGCATCTCGTCTTCGCCAAGGCCGAGGCGCTCACGCAGAAGCCGCGTCGTTTCAAGGCATTGC
TGGCGATAGGGGTCGCCCTTGTCCGAATAGCTTTTCGGAATGCCGTGATAGGAGGTGAGGATGACTTCGG
GCTTGAAGGAAAGCGTAGCCAGATGCTTTTCGACGGAACGCGCCAGCGCGTCGATATAGACCGGCTCGGC
CTCGTATGACGGCACGGTGCGGATTGCAGGCATGAAGCGCTTTTTCATCAGCGCCTCGAAGAACTTGTCG
TTCACCGTCGCCGTGGTGGTGGCGGAATATTGCGGATAGAGCGGGAATATCACAATCCGCTCGCAGCCCT
GCTGAAGCAGGCGGTCGGTTATGCTCTCGATGGAAGGCTGGCCATAGCGCATCGCCCAGTCCACCACGAC
ATTGGGTTGGTCGCTAAGTGCTTTGGCAAGTTTCTCGCCCTGCGCGCGCGTATAGGTGCGCAGGGGCGAT
TCATTATTCTCGTGATTCCAGATGCGATCATAGAGCCTGCCGGAACGCCTGGGCCGTGTGTTGAGAACAA
TACCGTAAAGGATCGGGTACCAGATAAGGCGCGACCATTCGATGACGCGGCGGTCGGAGAGAAATTCGGC
AAGATAGCGCCGCATCGGGCCGTAGCTCGTGCCGTCCGGCGTGCCGAGATTGACCAGCAAAACCCCGACT
TTCGGCAGTTTGGCCGATGTATGCAGCGGGGTCTGCGCTGTCTGGCTGACGTTCACACGCACCTTGTCCG
TTCCACTCA
Protein Sequence 
>gi|23499843|ref|NP_699283.1| ferrochelatase [Brucella suis 1330] MSGTDKVRVNVSQTAQTPLHTSAKLPKVGVLLVNLGTPDGTSYGPMRRYLAEFLSDRRVIEWSRLIWYPILYGIVLNTRPRRSGRLYDRIWNHENNESPLRTYTRAQGEKLAKALSDQPNVVVDWAMRYGQPSIESITDRLLQQGCERIVIFPLYPQYSATTTATVNDKFFEALMKKRFMPAIRTVPSYEAEPVYIDALARSVEKHLATLSFKPEVILTSYHGIPKSYSDKGDPYRQQCLETTRLLRERLGLGEDEMRATFQSRFGPEEWLQPYTDETVKELAKNGVKSVAVLNPGFVADCLETVDEIGNEAAEEFLENGGENFSHIPCLNDSEEGMKVIETLVRRELLGWV
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(UniProt: P0A3D7).

CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(UniProt: P0A3D7).

PATHWAY: Protoheme biosynthesis; last step(UniProt: P0A3D7).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P0A3D7).

SIMILARITY: Belongs to the ferrochelatase family(UniProt: P0A3D7).

MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme (Almirón et al., 2001).
COG COG0276H, under H: Coenzyme transport and metabolism
References
Almirón et al., 2001: Almirón M, Martínez M, Sanjuan N, Ugalde RA. Ferrochelatase is present in Brucella abortus and is critical for its intracellular survival and virulence. Infection and immunity. 2001; 69(10); 6225-6230. [PubMed: 11553564].