Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

virB1

Victor ID 970
Gene Name virB1
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1164506
NCBI Protein GI 23499836
Locus Tag BRA0069
Genbank Accession AE014292
Protein Accession NP_699276
Other Database IDs UniProtKB-ID: VIRB1_BRUSU
UniRef100: UniRef100_Q9RPY4
UniRef90: UniRef90_Q2YIT5
UniRef50: UniRef50_Q2YIT5
UniParc: UPI00000DCBB7
EMBL: AF141604
EMBL: AE014292
EMBL-CDS: AAD56611.1
EMBL-CDS: AAN33281.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0069
NMPDR: fig|204722.1.peg.2185
TIGR: BRA0069
HOGENOM: HBG742087
OMA: VFDPCRN
ProtClustDB: CLSK884272
BioCyc: BSUI204722:BR_A0069-MONOMER
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 66524
Gene Ending Position 67240
Gene Strand (Orientation) -
Protein Name type IV secretion system protein VirB1
Protein pI 9.83
Protein Weight 22720.07
Protein Length 238
Protein Note similar to GP:5929866, and GP:5929866; identified by sequence similarity; putative
DNA Sequence 
>gi|56968493:66524-67240 Brucella suis 1330 chromosome chromosome II, complete sequence
GTTAGAAAACAACTACGCCGTCCGTATTATCCTTCCCTGGCGGCGCATCGGTTGTCGCCGGCTGCTCTGT
CTTCAGCCTTACAGGCGCGTTCGCCGCGCCGGTGGCTGGCATCGACTGCGATGATGGATGCAGATCGACA
AGAACAGAATTACGATCAGCCGGCTGTCGTTTTTTTACTGGGGCAGCTACTGCTGCCGCCGCATCCGGCG
TTTTCCGGATCATGGGCACGACAGCAATCGGCTTTGTGGTCACGTCGGCGCTTGCCACGACCTTCTGCAC
GTAGCTGGGACTGCCGGGCTTCGTCTTGAAGCCGCCCGTAAAGTTGCCGGCGTAGTAACAGGAGAATGCG
GCGCGAAGCGCGCCTTGTTCCTGACCGGGCATCTTCACGATGGCCCGACGGTAGCAGTCTTCAAGGATCT
TGGATCCCATCTTCAGGTTCTTGCAGGGGTCGAACGCTTGTGCGTAGGTGCTGCCATATTTCGGCAGATT
GTACCTGTTGACTTGAGCAATACCCAAAGAGAAATTCCAGCCTTTGGCTTCCAGTGACTGTGCTGTCGTG
ATTGCTTCATCAAGGGAAACGGGTTGACGGACCAACCGCCCACCAACGACGCCTATTGCATAAGGATTGA
AGCCCGACTCGACCTGCACGATTGCTGCCATAGTCTGAGGTGCAACAGTCGGTGCGCATTGTTGCGCGAG
GACAAGGAATGGCACCA
Protein Sequence 
>gi|23499836|ref|NP_699276.1| type IV secretion system protein VirB1 [Brucella suis 1330]
MVPFLVLAQQCAPTVAPQTMAAIVQVESGFNPYAIGVVGGRLVRQPVSLDEAITTAQSLEAKGWNFSLGI
AQVNRYNLPKYGSTYAQAFDPCKNLKMGSKILEDCYRRAIVKMPGQEQGALRAAFSCYYAGNFTGGFKTK
PGSPSYVQKVVASADVTTKPIAVVPMIRKTPDAAAAVAAPVKKRQPADRNSVLVDLHPSSQSMPATGAAN
APVRLKTEQPATTDAPPGKDNTDGVVVF
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: The Brucella abortus virB operon, encoding a type IV secretion system (T4SS), is required for intracellular replication and persistent infection in the mouse model. The products of the first two genes of the virB operon, virB1 and virB2, are predicted to be localized at the bacterial surface. Both mutants were shown to be nonpolar, as demonstrated by their ability to express the downstream gene virB5 during stationary phase of growth in vitro. Both VirB1 and VirB2 were essential for intracellular replication in J774 macrophages. The nonpolar virB1 mutant persisted at wild-type levels, showing that the function of VirB1 is dispensable in the mouse model of persistent infection (Höppner et al., 2005).

A B abortus polar virB1 mutant failed to replicate in HeLa cells, indicating that the virB operon plays a critical role in intracellular multiplication (Höppner et al., 2005).

Polar mutations in the virB1 to virB2 intergenic region or in virB2 reduced the detection of VirB5 to a greater extent than they did that of VirB12. A virB1 mutation also eliminates the transcription of virB12 in B suis (Höppner et al., 2005).

An infection assay with signature-tagged Brucella abortus mutants demonstrated that mutagenesis of the virB1 gene causes attenuation of virulence (Höppner et al., 2005).
References
Höppner et al., 2005: Höppner C, Carle A, Sivanesan D, Hoeppner S, Baron C. The putative lytic transglycosylase VirB1 from Brucella suis interacts with the type IV secretion system core components VirB8, VirB9 and VirB11. Microbiology (Reading, England). 2005; 151(Pt 11); 3469-3482. [PubMed: 16272371].