Victors

virB4 from Brucella suis 1330
Victor ID	967
Gene Name	virB4 from Brucella suis 1330
Sequence Strain (Species/Organism)	Brucella suis 1330
NCBI Gene ID	1164503
NCBI Protein GI	23499833
Locus Tag	BRA0066
Protein Accession	NP_699273.1
Other Database IDs	UniProtKB-ID: VIRB4_BRUSU UniRef100: UniRef100_Q9RPY1 UniRef90: UniRef90_Q2YIT8 UniRef50: UniRef50_Q2YIT8 UniParc: UPI00000DD821 EMBL: AF141604 EMBL: AE014292 EMBL-CDS: AAD56614.1 EMBL-CDS: AAN33278.1 RefSeq_NT: NC_004311.2 GenomeReviews: AE014292_GR KEGG: bms:BRA0066 NMPDR: fig\|204722.1.peg.2182 TIGR: BRA0066 HOGENOM: HBG395345 OMA: ASHTEPG ProtClustDB: CLSK884268
Taxonomy ID	204722
Chromosome No	II
Gene Starting Position	62912
Gene Ending Position	65407
Gene Strand (Orientation)	-
Protein Name	type IV secretion system protein VirB4
DNA Sequence	>gi\|56968493:62912-65407 Brucella suis 1330 chromosome II, complete genome GTCACCTTCCTGTTGATTTGGACGACGCAATGCGTGCTTTCCGGCGCTCCTGGAACACCGGAAGCCATAC CGAAGGGTCGTCCCCGACTTCGGCAATGACCTGGTCCAGCAACTCGTTGTTGTCCGAGCTGCCTGAGAGG ATCGCCAGTTCGTCATCAAAGCCATTAAGCTCCAGGCGGCAAATCATCGAATGATGCCCCTGCTTGACCA GGAACATGCGGCTTTCCTCGCTCATACTGCGGATGATGTCGAACTCCTCGTTCGTCACCTTGAAGCCGTC CGTGTACTCGTGATAATCGGCTTTCGGATTCGGCAGATAGATTTCCGTTGCCACCTGTTGCACCAGGGCC GACGCGACTTTGGAGTTGAGCAGGCTGGAGGGCATTTGCGTGGCAAACACGCCCAGGCCGTTTTGCTTGC GGATGGTAAGCTGCTTGTTATTGGCGAACTCGCTGAATGCTTCGTCGTCTACCCACTTCCACGCCTCGTC CATCCAGTAGATGAAACGACGCCCGTCGATAGCCAATTCCATACGATGCAGCAGGTACATGGAAATCGGC GTACGCACGTCGGCGTTGTCGAGGAAGTCGGTGCCATCGAAACCGTAATTGCTATGCGTGTTGAAGTCGA TCTGGTCTTGCGGGCAATCCAGCACCCACCAGAATGTGCCCCGCTTGCCGGTGCCGTCATCAAAACACCA CTTCGCGAGCCGCTTCGCCACGCTGTTTTCACGATCTTGGCGGTCGCTGCCTTCGGTAACGTTTTGCAGC ACCGTCGACAGACGCCGCAGCTCCGGGCGCATGCGCATGACCGTACGTATGGCATGACTGATCCGGCTTT CGTCCGTTGTGGTCACGTGCTGGCCATCACGCGATACCAGTACCTGTACGAGTTTTTCAAGGAACAGGAT GTTCCCCGCTGTTGGCTCCATCTGGAATGGGTTGAACCCGGTGGGTTCGCCGTTCCTGATGGCAAGGTAT TTGCCGCCAATGGCAAGAATAGTCCCTTTCGCGCCTTGATCCTTGTCGAAAAAGACGTTGCACATACCCA TCGGAGCATTGTGCAAATACTTCTGCGCTTGCGCGAGGCAGAAGTTCATCAGGACAGTTTTACCAGCGCC GGACTGGCCGATGATGCGGGTATTGCCCAGAAGCTTCTTGTCGAAGTTGTCTTCGTCGCCTTTTGAATAG TGGAAGTTCAGGTAGGCGGGCTGTCCGGAAGGGGTTTTAAGCAGCGTCAATGCCTGCCCCCAGGGGTTGC CATCCCGCTTGCCTGCGCGGAAATTATGGAAACATGACAGACCGGCGAAGTTGAGCGAGGTCAGGCCCGC CACGCGCGGCCGATACCGCCAGTTACATGGCAGTTGTGCATAGAACGCCGCATCCGTGGCCGTGGCGATC ACCGTCGCCAGAAAACCATTGTCTTGCAGGATGTTCATCGCATGTGATGTGTTGTGGCGCACCGTCTCCA TGTCTTCGGCAAATACCAGCAGCGAATAATGATATTCACCCATGACGAAATCGCCCCGGCCGAGCTGGTC GATGGCAATGTCCATTTCCATGATCTGGGATGCGCTGCCGTCTTCGGTATTTTGGAGCCGCTGCTTTTGC CGTGTCAGGAACTCCTTGCCATCCCGCTTTGTCATGAAGCTGAAGGATTGTGTGATGACGTACTCGTAAT CGCTATACATCAGGCCGTTCAAAATGCCCGGCTCGGTGTGGCTCGCATAGTCCTTGAAATCGATGCCACG CGCATAGCGCGTCGCGTTCGCGGTGCGTATCTCGATGGTTTCAGTGCCAGCATACACCCACCCGGTGCCC AGATATTCATCCAGTGGGCAGGATGGCACGCGCACCTTCTGCCATTCGCCTGAGAGAAGGAAGTTATAAA ATTCAAGCTGCTGTGAGCACAGTGCGCCGTGTTTGTCCTCATAGGTGCTCAGCACTTCGATGCCACGCCC GTCATCGCCACCGTAGCGACGCAAGCTGGTTTCAATTTGATTGCCGATTTCGTCCAGCTTGCGGATGGCA AGCTGCTGCTCCTGTAAGATTTCCGCATGCGAACGATGAACGGCCACGTTGAACAACCTGCTGATCTTCG CCGGCACTGGCCGGTAAATAACCGTCAGATACAGTTCGTTTGACATCATTCGGTAGCCGGAAAACGATCC GTAATACTTCTTGTCGAGAGCGTCGCAGAACGGATTGGAAAAGAAGGATTTCAAGTGATCCCAGGTTCTG CGCCGTACGTTGTGGGACCACAGCGCCACATTGTTTGAGGCGATGGCACGGAACAAGGTGTTAAGCTGGT CCTTGCGGATCAACAGTTCCTCCTTGTCCTGCGTCTCGAAAGCCAGGCCCGCGATGCGCCATGTGCGCAC GAAATCACCGTCACGCGTTATCACGGTGGTCGGGCCAACCTGGCTCCTGAAGGGAATGAACGGTGCAAGT GACCGTTCATTATTGAGCTGTTGCGCGTATTTGGATTGAGCGCCCA
Protein Sequence	>gi\|23499833\|ref\|NP_699273.1\| type IV secretion system protein VirB4 [Brucella suis 1330] MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKLDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEIRTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHNAPMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQQVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQERRKARIASSKSTGR
Molecule Role	Virulence factor
Molecule Role Annotation	MUTATION: A mutant strain of B abortus that contains an in-frame deletion in virB4 is unable to replicate in macrophages and survives in mice (Kim et al., 2004). ntracellular replication was inhibited in wild-type B abortus after introducing a plasmid expressing a mutant VirB4 altered in the NTP -binding region. VirB4 containing the intact NTP -binding region is essential for evasion of fusion with lysosomes (11988518). The ruffling associated with internalization of the virB4 mutant results in a more rapid uptake than for the wild-type strain. The virB4 mutant shows primarily small regions of phalloidin staining at the sites of binding. Macrophages incubated simultaneously with B abortus and the fluid-phase marker tetramethyl rhodamine isothiocyanate (TRITC)-dextran accumulate the marker in large vacuoles containing the wild-type strain, but little or no marker accumulates in phagosomes containing the virB4 mutant. Similarly, phase-contrast micrographs have shown the wild-type strain in large phase-transparent compartments, but the virB4 mutant is in much smaller compartments (14738898). Intracellular growth-defective virB4 mutant and attenuated vaccine strain S19 did not induce abortion (Kim et al., 2004). The B abortus virB4 mutant was completely cleared from the spleens of mice after 4 weeks, while the pncA mutant showed a 1.5-log reduction of the number of bacteria isolated from spleens after 10 weeks. Splenomegaly was not observed at all in mice infected with virB4 mutant (Kim et al., 2004).
COG	COG3451U, under U: Intracellular trafficking, secretion, and vesicular transport
References	*Kim et al., 2004:* Kim S, Kurokawa D, Watanabe K, Makino S, Shirahata T, Watarai M. Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice. FEMS microbiology letters. 2004; 234(2); 289-295. [PubMed: 15135535].


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virB4 from Brucella suis 1330