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virB5 from Brucella suis 1330

Victor ID 966
Gene Name virB5 from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1164502
NCBI Protein GI 23499832
Locus Tag BRA0065
Protein Accession NP_699272.1
Other Database IDs UniProtKB-ID: VIRB5_BRUSU
UniRef100: UniRef100_Q9RPY0
UniRef90: UniRef90_Q2YJ75
UniRef50: UniRef50_Q2YJ75
UniParc: UPI00000DD820
EMBL: AF141604
EMBL: AE014292
EMBL-CDS: AAD56615.1
EMBL-CDS: AAN33277.1
RefSeq_NT: NC_004311.2
GenomeReviews: AE014292_GR
KEGG: bms:BRA0065
NMPDR: fig|204722.1.peg.2181
TIGR: BRA0065
HOGENOM: HBG531833
OMA: IQNEQTK
ProtClustDB: CLSK884267
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 62191
Gene Ending Position 62907
Gene Strand (Orientation) -
Protein Name type IV secretion system protein VirB5
DNA Sequence 
>gi|56968493:62191-62907 Brucella suis 1330 chromosome II, complete genome
GTTAATAGGCGGCTTCCAGTGCTTTCGGCTGCGGATAGCCGCGCCGGGCATTATCCTTCGCGTCTAATTC
GTGCTGGCGTTCGTCCAGGAGCTTGTCCTGTGCCTCTGCCATCTGCTGGTAGAGATGAAGGCGGGTGTCT
TCGTTTTGGATCATGGCATTTTCGCTTTCGATCCGGCCTTGCAATTCGGCAATCGCCTTCGGGTCTCCCG
TCTTGTTGATCTCCTGCATCAAGCTCTCGATCTCTTGTAGGCGTTTGTCAGTGGCGTCGTAGGCTTTGGA
CGTCATGACCTTGTCCTGGACCGGCTTCACCACTTTAGCCTCACATGCGATGCGCTGATCTTTGTCGGAG
ATGGAGGCACACGGATCGTATACCTGGCTTTTGCGTAAGAGGTTGGCCGTTTCGCCAGCCGCCGCCAGGT
AGCCGCCGCTCATCACCGCTTCGTAGAGATCTCGCCAGTTATGTGGCAGATAGCTACGCAGCGTAGGGTC
GCGGAGAATATCGCCAAGGCCACGGTAGCCGGTCAGGGCATCGAACTGCATTTTCTGTTCTTCAAACTGC
TGTTTCAGTTGCTCGATCTGCTGGGCGAACTTCACCATCTGTTCGAGATGGTTGGCCAGGTTCTGCGCGA
TGGAGCCGGCATCTGTCACCGGGAGCTGCGCGTGCGCCGTGCTGGTTACAGTCAGGGCGAATGCGAAGCT
GAGAATTATCTTCTTCA
Protein Sequence 
>gi|23499832|ref|NP_699272.1| type IV secretion system protein VirB5 [Brucella suis 1330] MKKIILSFAFALTVTSTAHAQLPVTDAGSIAQNLANHLEQMVKFAQQIEQLKQQFEEQKMQFDALTGYRGLGDILRDPTLRSYLPHNWRDLYEAVMSGGYLAAAGETANLLRKSQVYDPCASISDKDQRIACEAKVVKPVQDKVMTSKAYDATDKRLQEIESLMQEINKTGDPKAIAELQGRIESENAMIQNEDTRLHLYQQMAEAQDKLLDERQHELDAKDNARRGYPQPKALEAAY
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: A comparison of the VirB8 and VirB5 contents after induction of the B suis wild type and of virB5 and virB12 mutants further confirmed that the virB5 and virB12 genes belong to the same operon (O'Callaghan et al., 1999).

Smooth strains of Brucella unable to replicate (ie, killed B suis or the avirulent mutant B suis virB5) exhibit delayed phagosome-lysosome fusion (O'Callaghan et al., 1999).

Polar mutations in the operon upstream of virB5 exert a greater effect on the expression of virB5 than they do on the expression of the downstream gene virB12. It indicates that in B abortus , regulatory elements other than the virB promoter may influence VirB12 protein levels (O'Callaghan et al., 1999).

Four independent mutants in virB5, virB9 or virB10 were highly attenuated in an in vitro infection model with human macrophages (O'Callaghan et al., 1999).
References
O'Callaghan et al., 1999: O'Callaghan D, Cazevieille C, Allardet-Servent A, Boschiroli ML, Bourg G, Foulongne V, Frutos P, Kulakov Y, Ramuz M. A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Molecular microbiology. 1999; 33(6); 1210-1220. [PubMed: 10510235].