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virB9 from Brucella suis 1330

Victor ID 963
Gene Name virB9 from Brucella suis 1330
Sequence Strain (Species/Organism) Brucella suis 1330
NCBI Gene ID 1164498
NCBI Protein GI 23499828
Locus Tag BRA0061
Protein Accession NP_699268.1
Other Database IDs UniProtKB-ID: VIRB9_BRUSU
UniRef100: UniRef100_Q2YJ79
UniRef90: UniRef90_Q2YJ79
UniRef50: UniRef50_Q2YJ79
UniParc: UPI00000DC988
EMBL: AF141604
EMBL: AE014292
EMBL-CDS: AAD56619.1
EMBL-CDS: AAN33273.1
RefSeq_NT: NC_004311.2
TCDB: 3.A.7.3.1
GenomeReviews: AE014292_GR
KEGG: bms:BRA0061
TIGR: BRA0061
HOGENOM: HBG751000
OMA: YGPRNWR
ProtClustDB: CLSK884262
BioCyc: BSUI204722:BR_A0061-MONOMER
Taxonomy ID 204722
Chromosome No II
Gene Starting Position 59039
Gene Ending Position 59908
Gene Strand (Orientation) -
Protein Name type IV secretion system protein VirB9
DNA Sequence 
>gi|56968493:59039-59908 Brucella suis 1330 chromosome II, complete genome
GTCATTGCAGGTTCTCCCCGGGCGATTTGATGACGCGCTTGACGCCTGGCACTGTGGTTCCGTCGCGTGC
GGGAACGCCATCAGGATTGAAGGATTCGTTGTAGATGCCAATCACCTTGTTACCGAGGCGCAGCACCATT
TGCTTTGCGACCCGATGCAGGACCAGAATGTCCGGCGCTGACGGGTCTATATGGCTGTTCACGATGCTTT
CCGACTTGTCTTCGGCGACAAGGAAAGCGGCCGGAAAGTCTCTGTTGTTCGGAAAGCGCAAATATGTGAA
CCGCCCATCGTCATAGGCAAGAGTGGGCGCGATGCTGGCCGAATTAGTGCCGACCTGCATCGTGTAGTTC
CAGTTTACCGGCGACGGTCTGGCATTCATGCGGGCCTGTACCACTCGCTTTTGCGCGGCTGCTACTGCCG
CCGCTGCCGCTGCCGCCGGGTAGCGGAACTGAACACGATAGGCAACACGCTGATTGTTGCGGCCGGGCAT
CAAGCGCAAATCGAAATCGTACATGCGCTGATCTGTCGTGACCATCAGGTTTGTGTCCCACTTGCCCGGT
TCCGGGGCCATGTCGATCGATTGGTTCGAGTGGGAGAGCGTCATGGAGCGGGCCTTGAGATACAGGATGT
TGTGGCTCGCCTTGAACTCCCAGCCTTGCGTAAATCCTGAGGCGACATCCTCAATATTCTCGCCCGGCGC
AAACACGATGCGTGCGCCAACGCCGGGCAGGGCCCGAACCAGCACGACATCGCCGGAATTGTAATCGACA
TACTGAATTCGGCTGTCGTATTTTGAGCCGGACGGGATTTTGGTTGCCCAGGAGGGCGAGGCCAGCGTGA
TCAGTATGCACGCAAGCAGGAATCTTTTCA
Protein Sequence 
>gi|23499828|ref|NP_699268.1| type IV secretion system protein VirB9 [Brucella suis 1330] MKRFLLACILITLASPSWATKIPSGSKYDSRIQYVDYNSGDVVLVRALPGVGARIVFAPGENIEDVASGFTQGWEFKASHNILYLKARSMTLSHSNQSIDMAPEPGKWDTNLMVTTDQRMYDFDLRLMPGRNNQRVAYRVQFRYPAAAAAAAVAAAQKRVVQARMNARPSPVNWNYTMQVGTNSASIAPTLAYDDGRFTYLRFPNNRDFPAAFLVAEDKSESIVNSHIDPSAPDILVLHRVAKQMVLRLGNKVIGIYNESFNPDGVPARDGTTVPGVKRVIKSPGENLQ
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Uptake in the presence or absence of Ca2 and Mg2 did not influence the subsequent intracellular survival of wild-type Brucella, whereas the decrease in the number of surviving virB9 mutant cells was delayed in the absence of Ca2 and Mg2. Possibly two types of adhesion molecules promoted uptake of Brucella, one being Ca2 and Mg2 dependent and the other not, and that both types participate in the uptake of wild-type bacteria but only the latter type participates in the uptake of the virB9 mutant (Gorvel and Moreno, 2002).

Four independent mutants in virB5, virB9 or virB10 were highly attenuated in an in vitro infection model with human macrophages (Gorvel and Moreno, 2002).

The intracellular fate of three virB mutants (virB2, virB4 and virB9) in HeLa cells by immunofluorescence was examined. The three VirB proteins are not necessary for penetration and the inhibition of phago-lysosomal fusion within non-professional phagocytes. Rather, the virB mutants are unable to reach the replicative niche and reside in a membrane -bound vacuole expressing the late endosomal marker, LAMP1, and the sec61beta protein from the ER membrane, proteins that are present in autophagic vesicles originating from the ER (Gorvel and Moreno, 2002).

Attenuated non-polar virB2, virB4, virB8, virB9 and virB10 Brucella mutants are capable of penetrating cells as the same rate as the virulent wild-type Brucella, transit through EEA1 -positive early compartments and then localize in LAMP1-positive compartments at early times of infection (Gorvel and Moreno, 2002).
COG COG3504U, under U: Intracellular trafficking, secretion, and vesicular transport
References
Gorvel and Moreno, 2002: Gorvel JP, Moreno E. Brucella intracellular life: from invasion to intracellular replication. Veterinary microbiology. 2002; 90(1-4); 281-297. [PubMed: 12414149].