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miaA from Shigella flexneri 2a str. 2457T

Victor ID 944
Gene Name miaA from Shigella flexneri 2a str. 2457T
Sequence Strain (Species/Organism) Shigella flexneri 2a str. 2457T
NCBI Gene ID 1079793
NCBI Protein GI 30065543
Locus Tag S4594
Protein Accession NP_839714.1
Other Database IDs UniProtKB-ID: MIAA_SHIFL
UniRef100: UniRef100_P59197
UniRef90: UniRef90_A8AMN0
UniRef50: UniRef50_Q7U347
UniParc: UPI000012F0D1
EMBL: AB000785
EMBL: AE005674
EMBL: AE014073
EMBL-CDS: BAA22627.1
EMBL-CDS: AAN45743.1
EMBL-CDS: AAP19526.1
RefSeq_NT: NC_004337.1
RefSeq_NT: NC_004741.1
EnsemblGenome: EBESCG00000086881
EnsemblGenome_TRS: EBESCT00000087836
EnsemblGenome_PRO: EBESCP00000084658
EnsemblGenome: EBESCG00000088435
EnsemblGenome_TRS: EBESCT00000089391
EnsemblGenome_PRO: EBESCP00000086027
Geno
Taxonomy ID 198215
Gene Starting Position 4496229
Gene Ending Position 4497179
Gene Strand (Orientation) +
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|30061571:4496229-4497179 Shigella flexneri 2a str. 2457T, complete genome
GATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTGATGGGGCCGACGGCCTCCGGTAAA
ACGGCGTTAGCCATTGAGCTGCGTAAAATTTTACCAGTAGAGTTGATAAGCGTTGATTCTGTCCTTATTT
ACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCT
GGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCC
GATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGG
AAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTGCGGGCCAGAATTGAGCAACAGGCGGCAGAGCA
AGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAAT
GATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGC
AAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCT
CCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTT
TTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTT
ACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAA
GCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCG
CGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTG
Protein Sequence 
>gi|30065543|ref|NP_839714.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Shigella flexneri 2a str. 2457T] MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSVLIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARIHPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATRQLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: miaA mutants had reduced ability to invade and spread in MK2 cell monolayers compared to the wildtype (Durand et al., 1997).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Durand et al., 1997: Durand JM, Björk GR, Kuwae A, Yoshikawa M, Sasakawa C. The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes. Journal of bacteriology. 1997; 179(18); 5777-5782. [PubMed: 9294434].