Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

sepA from Shigella flexneri 2a str. 301

Victor ID 892
Gene Name sepA from Shigella flexneri 2a str. 301
Sequence Strain (Species/Organism) Shigella flexneri 2a str. 301
NCBI Gene ID 1238005
NCBI Protein GI 31983545
Locus Tag CP0070
Protein Accession NP_858203.1
Other Database IDs UniProtKB-ID: SEPA_SHIFL
UniRef100: UniRef100_Q8VSL2
UniRef90: UniRef90_Q8VSL2
UniRef50: UniRef50_Q84GK0
UniParc: UPI00000B9CB8
EMBL: Z48219
EMBL: AL391753
EMBL: AF348706
EMBL: AF386526
EMBL-CDS: CAA88252.1
EMBL-CDS: CAC05786.1
EMBL-CDS: AAK18385.1
EMBL-CDS: AAL72309.1
RefSeq_NT: NC_004851.1
MEROPS: S06.013
EnsemblGenome: EBESCG00000093242
EnsemblGenome_TRS: EBESCT00000094198
EnsemblGenome_PRO: EBESCP00000090405
GenomeReviews: AF386526_GR
KEGG: sfl:CP0070
HOGENOM: HBG467630
OMA: DIFNPAN
ProtClus
Taxonomy ID 198214
Plasmid No pCP301
Gene Starting Position 52744
Gene Ending Position 56838
Gene Strand (Orientation) -
Protein Name SepA, extracellular serine protease of the IgA1 protease family, secreted by a C-terminal autotransporter domain
DNA Sequence 
>gi|31983523:52744-56838 Shigella flexneri 2a str. 301 plasmid pCP301, complete sequence
GTCAGAAAACATAACGGAAGTTGGCGTTTATCGCATTATCCACATTATATTTCCCGAACGCTGATTTCTC
CAGCTCCAGTCCCAAACGCATATTATCCTTAATTTCCGCATTCATCCCTACCGTCATCAGCATCCTGCTA
TCTTTTTCACCTTCGAAACGTTTCTCTCCGGAAGCATCCTGCAGAACCGTTTCTCCGTTCGCCAGCAGGT
CGAACTGATAACCCAGCCCGGCTCGAGCTGTGATTTTCCAGTCGTCTCCGGAGAAGGCTCTTCCCACGTC
AACACCAGTACGGCCAATCAGTGGGTTATAATCCTTGTCTTTCATGCTCAGAGCCATTCCCCGGTCTTCC
CAGCTAAAAGCTTTTCCTGATACAGAACCGTAAACCAGCTCTATCTGTGGCTCCACCCAGGACTCTTTCG
TCAGGTGGTAACGATAACCAACTTCAGCACCGGCATACCAGGAATGAGAGCTGTAGTCTTTTGTTCCCAG
TGAGGCAAAATTCGCCGTGTGCTGATTATCATGATGGAGATATTTACCAATCAGGTCAAAATAAGCTCCG
GAATTAAAGAGTGCAGAGGCATACAGACCGCCACCCACGGATTTGTTTTTTCCACTGAATGCGTGGCTGC
TTGCATTGCTGTCCGTATAGGTCAGCAATGCCCCCGTAAATAAATCCACACCGTCCAGCTCATGCTTTCT
GTCGACACCAATCTGAACGTGAGTGTAGTTATCGCTGTAGTCACCATCTGCCGAACCGGTACCATTCATT
ATGCGTGCCCAGACACCGGCATCCCCCTGAGTATCCCGCAGGTCACCCATACGTTTGTTCAGGTTATTGA
CCTCTGTAAGGAAGGACTTATAACCTGATGCCATAAAGTCTTTTGCGGCTTTCGAGGCTCCGGCATCAGC
GGTAGTCTGATATCCTGTCAGCACCCATTTTGTGGCATCATCCGTTTTTTCCGTGCTGATTACTGGCGTA
ACGTTACTGAAACCAATCTGCTGCGTACCGGCAGTGAACGTTTTCTCATCACTCCCTTTCGGTGCGGTAA
TTAACGTCACGCTCATTTCACTGGCTGGTTTGTTTAAAAAGTCAACCAGGACGCTATTATTACTGCCTGA
CAGTTTGTCTGTGACATTCAACTGGTCAGCCCGGCTGTTGTTGGTATGCATCAGGAACGTGGTATTATCT
GCAGAAAGCTCTTTTACTTGTAAAGTGGCAAAGTGTTTCCCGTCCCCCAGTTCAATTCGCGAGCCCTTAG
CCTTCAGTGCACCGGCCTCTGATTTTCCGTCAGAGTACCACAGAGCATTGATCATATTTACCGTACTCTG
TGCCCCCGTAATACTGCCTCTCAGAACAGCGTTATATCCCTTCATTAAGGAGCTGAATAACGGAGAGTCC
GTCTTCCCTGCATCAGCATCACTGTCTCCCAGATTAATGGTTGCCGCAGTGGTTCCTGCATGAATATCCG
AACTCAGATAGCCCATATTTTTAACAGAAAAGCTGGCGTTATCTTCGAGGTTAATCCCTTCTGTTGTAGA
AATTACGGGTGAGTAATACCCCTGTTTCTGCGCAGAAGGCATCCCCGTCAGGGTCAGAGAAGCATTTTCT
TTTACATCCACAGCCCCGGTGCTGAAAATACCGCTCTGAGCCGTAAGTTTTGCTCCTTTTCCGAGGGTCA
GGCTGCTGTTGACAAAGCTGCCAACACGGTCAAAAACAGCATTCTGAGAGGTCACACTCACGGTGCTATC
ATAAGCCTCAATACCACCGGTGAATCTCTCCATGATTTGCAGGGATGATTTATTTTCCAGCGTAACATTT
CCGCTGTAATCACTCTGGTCACCAACTGTGGTTGCAGTGGCACTCCCTTCAACAGAGAAGACCGCATTAC
CGGTTCCGTCACTCCTGTCAGTAAAGACACGGCTACTTCCCAGAATGACTGTACTTCCATTGGTGGCGTT
AATATCTCCCTGCATCTTGCCGTTTCTGGACAGATGGAATGTTGCCGCATCGAGTTTCAGTTTTTCCAGC
GTAAACTGACGGGTTTCCCAGTCGCTCTGGCTGGAAGAAGTCGTCGTTCCAGCATGAATAACTGGATGAC
CCTGGAATATCAGCGTGCCGGACTTCTTGGTTATCGAACCTTCTGGCAGGTTAACTGAACCATCAAGAGC
CAGTACATCCTTACCTGAGAGCTGCGGAATATTCACATCCATATTGCCGTTTAGTTGGCCGTGATACATA
TAAGGCTGGCTGCTGGCCTCAATTTTTTGTTGCTTCACAGTATCAATGGCTTTATTACGATCTTTTCCGA
CATTCTGCCAGACACTGCTGTTGTTCAGATCGGAGAAGAACGGGGAATATGAACTCGCTTTCAGGATGAA
ATATTGCTTGGTAGAACTGTCATAATATAGGTCTCCCGGCGCTCCTCTCCCCCCAAAAATACTGACAGTA
TTAACCGGTACATTGATGTCACTGGCTTTTAATGTCTGAAGGTCGAGTGTTACCGTTGATTTTTTCTCAC
TGTTATTGGTAATGATGGCCCCGTAGTCAGCCGCCTGAAGACGGGTAAAGGTCAGATTATTACCATTCAG
TTCCAGACGTCCTCCACGGTATCCCCACGAAATATTATCCGGATTAACTTGCTGTGAGTCAGAAAGCACA
ACTGTCGGACGACCGCTGGCAATACCAACAGAGCTGAAAGCCTGTACCTTTCCGTCAGCATCCGCCTGCT
GGTTGAGAATAACGGTACCGTCCCCCACCTTGAGTCCGCCTGCATTCACACCGGTCCCGTTTACTGTCAG
GGTACCTTCGCCAGTTTTATGCAGGTTATCACCGGCAACTCCGTTCACCTGCCACAGAACGTGGGTTCCT
TTATCGGTGATAATCCCGCCCCCCATCCATGTCTGGCCGTTCAGGGCGGACACCCTGTAATCGCCGGCAA
ACTGCAGATACCCAGCTCCCTGATTAACGGAATTCTGCAGAACGACCTCGCCATTATTGCCGGTGAACAG
GAGGTTTTTTCCTGCGTTCAGATCATTTCCTTTCTTTCCATGCATATCCCAAGTCGTGTTTCCCTGAGTC
AGTGTGCCGGTGCCATTAGCCGCATCATATTTCCACTGCAGTACTCCTTCACCAGAGGTGTAGGCTATAG
TTTTGTCAAAATCATTTTGCGGTTGCTGACCAAGGAAATCCTGAGTGGTGACGACCCAGTTATTTCCATA
GTTAGTTCCTGAGGAAAGCACGCCAATCAGTACCCATTTTTTTTGCAGGGAATCATAAGCAAACAGGGGG
GAGCCGCTGTCCCCCATATTCAGATAATTAGCCAGAGGTCCATTCGCGGGATTAAAAATGTCACCGGTCT
GAGCTGTAATCATCTGTCCGTTGTTATAGGAGTTCAGCGCGCTGACGGTTCCCCCAACAAGAAATCCGCC
ATTGGTATACACTTGGGTGCGATTACCATTTTTGTCTTTGACGTACTGCATCCCCCCCCCAAGGCGATAG
AATTCAGTAAAGCGTCCGCCGGCCTGATAGGCACCGCTCACAGCCCCGATGTCAGACACTTCTGCCGGCG
CCACCTCTGTCACCAGTTTACTCAGGCGGCGGGTTTTAATATCAAGACCGCTATTATTATTTGTACCAAC
CGCAGTATAGGTGTTTTTGGCGTATCCGAAGCTCATCGTGTCAGAACCGCTGACATGTTTGGCGGTAACC
ACATACTGAGGGGAGTAAAGTGTATGGTCTCCCGGAGGCAGGCTACCGGTAGTAATAGTGGCACTACTGA
AATCAGCCATCGGGGCTTTATCAAGTTTACCGACCAGATTGCCCTGCTTGTCGTAAATGGAAATATTCGT
GGTCCCCGGCGTAAACTGACCTTTATTCTCGGCAAAGTCGCGGAATATCTGATAAGGGATCTCTGCACTG
ACCGTTGCTGACAGAGCTGGCCATGCCGAGGAAAGTGATAAAGCTGCAACAGACGTAAGAATAACCCGAC
GTGAAAGTCTGCGATGGCTTTTGCAGGTAACCCTGCGGGCCAGTTCGGAGACAGCAATCAGGCTTTTGGT
TATATGGCAATACTTAAGATAATATATTTTGTTCA
Protein Sequence 
>gi|31983545|ref|NP_858203.1| extracellular serine protease SepA [Shigella flexneri 2a str. 301] MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVAALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAANGTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYDSSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQLNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVFSVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNTTFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGALLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLGTKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: sepA mutant has attenuated virulence in ligated rabbit ileal loop model (Benjelloun-Touimi et al., 1995).
COG COG3468MU, under M: Cell wall/membrane/envelope biogenesis; U: Intracellular trafficking, secretion, and vesicular transport
References
Benjelloun-Touimi et al., 1995: Benjelloun-Touimi Z, Sansonetti PJ, Parsot C. SepA, the major extracellular protein of Shigella flexneri: autonomous secretion and involvement in tissue invasion. Molecular microbiology. 1995; 17(1); 123-135. [PubMed: 7476198].