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spa32 from Shigella flexneri 2a str. 301

Victor ID 890
Gene Name spa32 from Shigella flexneri 2a str. 301
Sequence Strain (Species/Organism) Shigella flexneri 2a str. 301
NCBI Gene ID 1238001
NCBI Protein GI 31983541
Locus Tag CP0151
Protein Accession NP_858284.1
Other Database IDs UniProtKB-ID: SPAN_SHIFL
UniRef100: UniRef100_P0A1K5
UniRef90: UniRef90_P0A1K5
UniRef50: UniRef50_P0A1K5
UniParc: UPI0000135D9E
EMBL: M81458
EMBL: D13663
EMBL: AL391753
EMBL: AF348706
EMBL: AY206439
EMBL: AF386526
EMBL-CDS: AAA26542.1
EMBL-CDS: BAA02827.1
EMBL-CDS: CAC05826.1
EMBL-CDS: AAK18470.1
EMBL-CDS: AAP79014.1
EMBL-CDS: AAL72305.1
RefSeq_NT: NC_002698.1
RefSeq_NT: NC_004851.1
EnsemblGenome: EBESCG00000093246
EnsemblGenome_TRS: EBESCT00000094202
EnsemblGenome_PRO: EBESCP00000090409
GenomeR
Taxonomy ID 198214
Plasmid No pCP301
Gene Starting Position 130936
Gene Ending Position 131814
Gene Strand (Orientation) +
Protein Name Spa32, secreted by and component of the Mxi-Spa machinery
DNA Sequence 
>gi|31983523:130936-131814 Shigella flexneri 2a str. 301 plasmid pCP301, complete sequence
TATGGCATTAGATAATATAAACCTAAATTTTAGTAGTGACAAACAGATAGAAAAATGTGAGAAACTATCT
TCAATAGATAATATTGATAGTCTCGTTTTGAAGAAGAAGAGGAAGGTTGAAATTCCTGAGTACTCTTTAA
TTGCATCTAACTATTTTACTATTGATAAGCACTTTGAACATAAGCATGATAAAGGAGAAATTTATAGTGG
CATTAAAAATGCGTTCGAACTTAGAAACGAACGAGCGACATATTCTGATATTCCAGAATCAATGGCCATT
AAAGAAAATATTTTGATACCAGATCAAGATATCAAAGCAAGGGAAAAAATAAATATCGGCGATATGAGGG
GGATCTTTTCATATAATAAGAGTGGAAATGCAGACAAGAACTTCGAAAGAAGTCATACTTCTTCTGTAAA
CCCTGATAATCTGCTAGAATCTGATAATAGAAATGGTCAAATTGGTTTAAAAAATCATAGCTTGTCTATT
GATAAGAATATTGCTGACATCATTTCTTTACTAAATGGAAGTGTTGCTAAATCATTTGAGCTGCCTGTAA
TGAATAAAAATACTGCAGACATAACCCCATCCATGTCATTGCAAGAAAAATCAATAGTTGAAAATGATAA
AAATGTTTTTCAAAAAAATAGTGAAATGACTTACCACTTTAAACAGTGGGGGGCTGGACATTCTGTTAGT
ATTTCAGTGGAGTCTGGTTCTTTTGTTCTAAAACCGTCAGATCAATTTGTAGGAAATAAACTTGACTTAA
TTTTGAAACAAGATGCTGAGGGTAATTACAGATTTGATAGCAGTCAACATAATAAGGGGAATAAAAATAA
TAGTACAGGATATAATGAACAGAGTGAAGAAGAATGCTA
Protein Sequence 
>gi|31983541|ref|NP_858284.1| Mxi-Spa secretion machinery protein Spa32 [Shigella flexneri 2a str. 301] MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEYSLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQDIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHSLSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSEMTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKGNKNNSTGYNEQSEEEC
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: spa32 mutants are unable to invade HeLa cells and do not secrete type III effectors (Tamano et al., 2002).
References
Tamano et al., 2002: Tamano K, Katayama E, Toyotome T, Sasakawa C. Shigella Spa32 is an essential secretory protein for functional type III secretion machinery and uniformity of its needle length. Journal of bacteriology. 2002; 184(5); 1244-1252. [PubMed: 11844752].