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pfoA

Victor ID 853
Gene Name pfoA
Sequence Strain (Species/Organism) Clostridium perfringens str. 13
NCBI Gene ID 988404
NCBI Protein GI 18309145
Locus Tag CPE0163
Genbank Accession BA000016
Protein Accession NP_561079
Other Database IDs UniProtKB-ID: TACY_CLOPE
UniRef100: UniRef100_P0C2E9
UniRef90: UniRef90_Q0TUS0
UniRef50: UniRef50_Q0TUS0
UniParc: UPI000013686D
EMBL: M81080
EMBL: BA000016
EMBL-CDS: AAA23271.1
EMBL-CDS: BAB79869.1
RefSeq_NT: NC_003366.1
PDB: 1M3I
PDB: 1M3J
PDB: 1PFO
PDB: 2BK1
PDB: 2BK2
TCDB: 1.C.12.1.1
GenomeReviews: BA000016_GR
KEGG: cpe:CPE0163
HOGENOM: HBG337470
OMA: GAYVAQF
ProtClustDB: CLSK877006
BioCyc: CPER195102:CPE0163-MONOMER
Taxonomy ID 195102
Gene Starting Position 209027
Gene Ending Position 210529
Gene Strand (Orientation) +
Protein Name perfringolysin O
Protein pI 7.62
Protein Weight 51761.69
Protein Length 500
Protein Note similar to sp:TACY_CLOPE PERFRINGOLYSIN O PRECURSOR (THETA-TOXIN) (THIOL-ACTIVATED CYTOLYSIN) from Clostridium perfringens (500 aa); 99.8% identity in 500 aa overlap. Putative N-terminal signal sequence was found by PSORT
DNA Sequence 
>gi|18308982:209027-210529 Clostridium perfringens str. 13, complete genome
TATGATAAGATTTAAGAAAACAAAATTAATAGCAAGTATTGCAATGGCTTTATGTCTGTTTTCTCAACCA
GTAATCAGTTTCTCAAAGGATATAACAGATAAAAATCAAAGTATTGATTCTGGAATATCAAGCTTAAGTT
ACAATAGAAATGAAGTTTTAGCTAGTAATGGAGATAAAATTGAAAGTTTTGTTCCAAAGGAAGGTAAAAA
GACTGGTAATAAATTTATAGTTGTAGAACGTCAAAAAAGATCCCTTACAACATCACCAGTAGATATATCA
ATAATTGATTCTGTAAATGACCGTACATATCCAGGAGCATTACAACTTGCAGATAAAGCCTTTGTGGAAA
ATAGACCTACAATCTTAATGGTAAAAAGAAAGCCTATTAACATTAATATAGATTTACCAGGATTAAAGGG
TGAAAATAGTATAAAGGTTGATGATCCAACCTATGGAAAAGTTTCTGGAGCAATTGATGAGTTAGTGTCT
AAGTGGAATGAAAAGTATTCATCTACACATACTTTACCAGCAAGAACTCAATATTCAGAATCTATGGTTT
ATAGTAAATCACAAATATCAAGTGCCCTTAATGTTAATGCTAAAGTCCTTGAAAACTCACTTGGAGTAGA
CTTTAATGCAGTAGCAAACAATGAGAAAAAAGTTATGATTTTAGCATATAAACAAATATTCTATACAGTA
AGTGCAGACTTACCTAAGAATCCATCAGATCTTTTTGATGACAGTGTTACATTTAATGATTTAAAACAAA
AGGGAGTAAGTAATGAAGCACCTCCACTTATGGTTTCAAATGTAGCTTATGGAAGAACTATATATGTTAA
GTTAGAAACTACTTCTAGTAGTAAAGATGTACAAGCTGCTTTCAAAGCTCTTATAAAGAACACTGATATA
AAAAATAGTCAACAATATAAAGATATTTATGAAAATAGTTCCTTCACAGCAGTAGTTTTAGGAGGAGATG
CACAAGAACATAACAAAGTTGTAACTAAGGACTTTGATGAAATAAGAAAAGTAATTAAAGACAATGCAAC
TTTTAGTACAAAAAACCCAGCATATCCAATATCTTATACTAGTGTTTTCTTAAAAGATAACTCAGTTGCT
GCTGTTCACAATAAAACAGATTATATAGAAACAACTTCTACAGAGTATTCTAAGGGAAAAATAAACTTAG
ATCATAGTGGAGCCTATGTTGCACAGTTTGAAGTAGCATGGGATGAAGTTTCATATGACAAAGAAGGAAA
TGAAGTTTTAACTCATAAAACATGGGATGGAAATTATCAAGATAAAACAGCTCACTATTCAACAGTAATA
CCTCTTGAAGCCAATGCAAGAAATATAAGAATAAAGGCAAGAGAGTGTACAGGTCTTGCTTGGGAATGGT
GGAGAGATGTTATAAGTGAATATGATGTTCCATTAACAAATAATATAAATGTTTCAATATGGGGAACTAC
TTTATACCCTGGATCTAGTATTACTTACAATTA
Protein Sequence 
>gi|18309145|ref|NP_561079.1| perfringolysin O [Clostridium perfringens str. 13]
MIRFKKTKLIASIAMALCLFSQPVISFSKDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKK
TGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKG
ENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVD
FNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVK
LETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNAT
FSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGN
EVLTHKTWDGNYQDKTAHYSTVIPLEANARNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTT
LYPGSSITYN
Molecule Role Virulence factor
Molecule Role Annotation TOXIN: perfringolysin O is a pore forming toxin that kills eukaryotic cells by making holes in the cell membrane by attaching to cholesterol (Rossjohn et al., 1997).
References
Rossjohn et al., 1997: Rossjohn J, Feil SC, McKinstry WJ, Tweten RK, Parker MW. Structure of a cholesterol-binding, thiol-activated cytolysin and a model of its membrane form. Cell. 1997; 89(5); 685-692. [PubMed: 9182756].