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pldA |
| Victor ID |
840 |
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Gene Name |
pldA |
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Sequence Strain (Species/Organism) |
Campylobacter jejuni subsp. jejuni NCTC 11168 |
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NCBI Gene ID |
905643
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NCBI Protein GI |
218562960
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Locus Tag |
Cj1351 |
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Genbank Accession |
AL111168 |
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Protein Accession |
YP_002344739 |
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Other Database IDs |
UniProtKB-ID: Q0P8Q8_CAMJE
UniRef100: UniRef100_D3FNJ8
UniRef90: UniRef90_A1W0W7
UniRef50: UniRef50_A8FN29
UniParc: UPI00000C1ED5
EMBL: AL111168
EMBL-CDS: CAL35463.1
RefSeq_NT: NC_002163.1
GenomeReviews: AL111168_GR
KEGG: cje:Cj1351
HOGENOM: HBG564477
OMA: LYLAYTQ
ProtClustDB: CLSK879211
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Taxonomy ID |
192222
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Gene Starting Position |
1282932 |
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Gene Ending Position |
1283921 |
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Gene Strand (Orientation) |
+ |
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Protein Name |
phospholipase A |
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Protein pI |
7.65 |
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Protein Weight |
37985.08 |
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Protein Length |
329 |
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Protein Note |
Original (2000) note: Cj1351, pldA, probable phospholipase A, len: 329 aa; highly similar to TR:O32349 (EMBL: Y11031) Campylobacter coli phospholipase A (297 aa),fasta scores; opt: 1544 z-score: 1835.1 E(): 0, 75.2% identity in 298 aa overlap, and similar to e.g. PA1_ECOLI phospholipase A1 precursor (EC 3.1.1.32) (289 aa), fasta scores; opt: 467 z-score: 560.5 E(): 6.4e-24, 34.7% identity in 262 aa overlap. 25.5% identity to HP0499. Contains probable N-terminal signal sequence~Updated (2006) note: Pfam domain PF02253 Phospholipase A1 identified within CDS. Further support given to product function. Characterised within Campylobacter coli, so putative not added to product function (Grant et al., 1997). Functional classification -Pathogenicity~PMID:9119448, PMID:8300539 |
|
DNA Sequence |
>gi|15791399:1282932-1283921 Campylobacter jejuni subsp. jejuni NCTC 11168 chromosome, complete genome
AATGAGAAAAATTGCTTTATTTTTAAGCTTATGCGTTTTTATATGGGCTAGTGATTTACAACAAGCTTTA
GAGTATGAAAAACAAGGCGATTATAAAAAAGCTATGGAAATTTACAAAAAACTCGCTTTAAAAAATTCAT
CTGTTTTGATTTCTCAAGAACAAAATAACTCAAGTCAAGCAACACAAACGCAAAATTCTATCACTATAAA
AAAAGAAGAAAAACAAGACTTTTCTCGCTTAGCTTTGGCTAATTATCTTGGTGAAAATGAAAGCTTTAAT
CCCCTTGGCATTAGCTCTTATAAGATGAATTATTTTTTACCTTTTGCTTATAGTTTTAATTCTTTAGGAG
TAAATAATAATAAGAGTGAGGCGAAATTCCAACTTAGTGTTAAAAAAAGACTTTTTGAAAATTTACTAGG
ACTAGATGAAAAATATTATATAGCCTATACTCAAACTTCTTGGTGGCAAATTTATGAGCATTCTTCTCCT
TTTAGAGAAACCAACTATCAACCAGAATTTTTTATAGATCTTCCTTTATATCTTAAAGATTATGAATTTT
TTAACAATCTACGCGTAGGTATATTGCATGAAAGCAATGGCAAGGGAGATGAGAATTTACAATCTCGCTC
ATGGAATAGAATTTATGTATCTACTGCTATTTTATATAATAAATTTTTATTTGTTCCACGCCTTTGGTAT
AGAATTCCTGAAAACAAAAAAGATGATGATAATCCTGCCATCTTGCATTATATGGGAAATTTTGATGTGA
ATTTAGCGTATTTAGGTGATGATTATTTTATCAATCTTATGCTAAGAAATAATCTTAAATTCCACAATAA
CAAAGGCGCTATACAAGTTGATCTAGGATATGATATTTTTAATAACGGAATTTATTGGTATTTACAATAC
TTTAATGGCTATGGAGAAAGCCTTATAGACTATAACAAACACTTGCAAAGACTTTCCACTGGATTTTTGA
TTTCTTACTA
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Protein Sequence |
>gi|218562960|ref|YP_002344739.1| phospholipase A [Campylobacter jejuni subsp. jejuni NCTC 11168]
MRKIALFLSLCVFIWASDLQQALEYEKQGDYKKAMEIYKKLALKNSSVLISQEQNNSSQATQTQNSITIK
KEEKQDFSRLALANYLGENESFNPLGISSYKMNYFLPFAYSFNSLGVNNNKSEAKFQLSVKKRLFENLLG
LDEKYYIAYTQTSWWQIYEHSSPFRETNYQPEFFIDLPLYLKDYEFFNNLRVGILHESNGKGDENLQSRS
WNRIYVSTAILYNKFLFVPRLWYRIPENKKDDDNPAILHYMGNFDVNLAYLGDDYFINLMLRNNLKFHNN
KGAIQVDLGYDIFNNGIYWYLQYFNGYGESLIDYNKHLQRLSTGFLISY
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
MUTATION: a mutation in the pldA gene impairs the ability of C. jejuni to colonize the cecum (Ziprin et al., 2001) |
| References |
Grant et al., 1997: Grant KA, Belandia IU, Dekker N, Richardson PT, Park SF. Molecular characterization of pldA, the structural gene for a phospholipase A from Campylobacter coli, and its contribution to cell-associated hemolysis. Infection and immunity. 1997; 65(4); 1172-1180. [PubMed: 9119448].
Ziprin et al., 2001: Ziprin RL, Young CR, Byrd JA, Stanker LH, Hume ME, Gray SA, Kim BJ, Konkel ME. Role of Campylobacter jejuni potential virulence genes in cecal colonization. Avian diseases. 2001; 45(3); 549-557. [PubMed: 11569726].
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