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pseA |
| Victor ID |
830 |
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Gene Name |
pseA |
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Sequence Strain (Species/Organism) |
Campylobacter jejuni subsp. jejuni NCTC 11168 |
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NCBI Gene ID |
905608
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NCBI Protein GI |
218562927
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Locus Tag |
Cj1316c |
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Genbank Accession |
AL111168 |
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Protein Accession |
YP_002344706 |
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Other Database IDs |
UniProtKB-ID: Q0P8U1_CAMJE
UniRef100: UniRef100_A3ZC50
UniRef90: UniRef90_Q939J7
UniRef50: UniRef50_Q5HT89
UniParc: UPI00000C1EB8
EMBL: AL111168
EMBL-CDS: CAL35430.1
RefSeq_NT: NC_002163.1
GenomeReviews: AL111168_GR
KEGG: cje:Cj1316c
HOGENOM: HBG740439
OMA: CVISNQR
ProtClustDB: CLSK879200
BioCyc: CJEJ192222:CJ1316C-MONOMER
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Taxonomy ID |
192222
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Gene Starting Position |
1244255 |
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Gene Ending Position |
1245391 |
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Gene Strand (Orientation) |
- |
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Protein Name |
pseudaminic acid biosynthesis PseA protein |
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Protein pI |
7.65 |
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Protein Weight |
41405 |
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Protein Length |
378 |
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Protein Note |
Original (2000) note: Cj1316c, unknown, len: 378 aa; similar to e.g. TR:P72140 (EMBL: U50396) Pseudomonas aeruginosa WBPG (lipopolysaccharide gene cluster) (376 aa), fasta scores; opt: 257 z-score: 302.0 E(): 1.6e-09,23.5% identity in 366 aa overlap. No Hp match. Also similar to Cj1324 (33.2% identity in 373 aa overlap)~Updated (2006) note: Prosite domain PS00761 SPASE_I_3, Peptidase S26A, signal peptidase I identified within CDS. Product function modified to more specific family member based on motif match. PseA has been proposed to play a role in the pseudaminic acid biosynthetic pathway by appearing to synthesize pseudaminic acid (PseAm) directly from PseAc by transfer of an acetamidino group (Thibault et al., 2001). Plays a direct role in CMP-Pse5NAc7NAc or CMP-Pse5NAc7Am biosynthesis or transfer. Logan (Susan.Logan@nrc-cnrc.gc.ca) personal communication. Identifed as part of Campylobacter jejuni O-linked glycosylation locus (Cj1293 - Cj1342). Functional classification - Surface structures~PMID:16573682, PMID:12781527, PMID:16684771 |
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DNA Sequence |
>gi|15791399:1244255-1245391 Campylobacter jejuni subsp. jejuni NCTC 11168 chromosome, complete genome
ATCATTTTAAAACACACTCGTCTTTTCTTACCAAAGAACCATCATAATCTCTTATAAATTTACCATTTTC
ATCACGTTTAAAAATTTTTTTATTGGTAAAAGAATCTACTATTTTTTGAAATTCTTCCTCGCTAAAACCA
CTGAATTCAAGGTATTTTTTAATAGCCTTTTTAGGGGGCTTGCCATCGTATTTTTGTGCCAAACGCACCC
CTTCTTCACGGCTGATATAACCTAAACGAATATCCAAACAAGCATTATCTGTTGCTCTACCAAAGCCGTA
TTTGCAGTATTTTAAATAATCATGCACATGATTAGAATAACAATCTAAATTTTCAAAATTTTCATAAGTA
GTCTCAACAGGATGATCTAGAGTTAAAAAACCATATTTTTTAGAAATTTCAAGAATTTTTTTATAATCCC
ATTTAAAATAATACCCTAAAAATAGCCCCGTAACCCCTACTCTTTGAAGTTCTTCATCGCTTGGATAAGT
ATATAAAAACAAATCTTTTTCAGTGATACCATTTACCCCAAGCATATCAGAAGCACGATTTCCTAAAAGC
CCGCCAAATTCTTCAAGCCACTCTCTGCCTAAAATATTTTTATTTTTACTACTCGCAGGACCGCCATACT
CTATCTGCGGACTTTCTCCCCAGATGATCAAAGGCACTCCAAAATTTACCGCTATGCGTGGAACAGAAGT
AAAAATTCCCAAATGGTTTTGCCACTCATTATCCCCTGTTCTTATAAAAGCTTCACGGGCAAGTTTTTGA
TAAACTTTTGGATCACGCTTGATATGGATCAAATCCACGCCAAGATGATTTAAATTGTCTAAATTTTTGC
GCCCTATTTTCGTAGGTACGCTAGGCTCAAAACACACACACAAAGGATTAAGTCCAAGTTCAAGCATTTT
AACCACTTGAAAAGTAGAATCTTTTCCGCCACTTACTCCGATAACACAATCATAAACAGGATGTTTTTTG
TATTTTTTTATAAGCTCAAAAAATTCTTTCTCTCTTTCTTGCCAATTAATATTTTGATTTTTAGCTTCTT
GAGAACGACAAGCATCACAAACACCTTCTTCATCAAAATGTAAATCAGGCTTAGTATCTGGCATCACACA
TTTTTTACAAAATTTCA
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Protein Sequence |
>gi|218562927|ref|YP_002344706.1| pseudaminic acid biosynthesis PseA protein [Campylobacter jejuni subsp. jejuni NCTC 11168]
MKFCKKCVMPDTKPDLHFDEEGVCDACRSQEAKNQNINWQEREKEFFELIKKYKKHPVYDCVIGVSGGKD
STFQVVKMLELGLNPLCVCFEPSVPTKIGRKNLDNLNHLGVDLIHIKRDPKVYQKLAREAFIRTGDNEWQ
NHLGIFTSVPRIAVNFGVPLIIWGESPQIEYGGPASSKNKNILGREWLEEFGGLLGNRASDMLGVNGITE
KDLFLYTYPSDEELQRVGVTGLFLGYYFKWDYKKILEISKKYGFLTLDHPVETTYENFENLDCYSNHVHD
YLKYCKYGFGRATDNACLDIRLGYISREEGVRLAQKYDGKPPKKAIKKYLEFSGFSEEEFQKIVDSFTNK
KIFKRDENGKFIRDYDGSLVRKDECVLK
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
MUTATION: Mutation of Cj1316c or pseA had been shown to result in loss of PseAm. C jejuni that produced flagella lacking PseAm failed to autoagglutinate. Mutants (81-176) defective in autoagglutination showed a modest reduction in adherence and invasion of INT407 cells (Guerry et al., 2006) |
| References |
Guerry et al., 2006: Guerry P, Ewing CP, Schirm M, Lorenzo M, Kelly J, Pattarini D, Majam G, Thibault P, Logan S. Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence. Molecular microbiology. 2006; 60(2); 299-311. [PubMed: 16573682].
Thibault et al., 2001: Thibault P, Logan SM, Kelly JF, Brisson JR, Ewing CP, Trust TJ, Guerry P. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni flagellin. The Journal of biological chemistry. 2001; 276(37); 34862-34870. [PubMed: 11461915].
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