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pglF

Victor ID 822
Gene Name pglF
Sequence Strain (Species/Organism) Campylobacter jejuni subsp. jejuni NCTC 11168
NCBI Gene ID 905411
NCBI Protein GI 218562734
Locus Tag Cj1120c
Genbank Accession AL111168
Protein Accession YP_002344513
Other Database IDs UniProtKB-ID: Q0P9D4_CAMJE
UniRef100: UniRef100_Q5HTY4
UniRef90: UniRef90_Q5HTY4
UniRef50: UniRef50_A7I1W9
UniParc: UPI00000C1E26
EMBL: AL111168
EMBL-CDS: CAL35237.1
RefSeq_NT: NC_002163.1
GenomeReviews: AL111168_GR
KEGG: cje:Cj1120c
HOGENOM: HBG429349
OMA: PFPRSVI
ProtClustDB: CLSK879094
BioCyc: CJEJ192222:CJ1120C-MONOMER
Taxonomy ID 192222
Gene Starting Position 1052029
Gene Ending Position 1053801
Gene Strand (Orientation) -
Protein Name UDP-GlcNAc C4,6 dehydratase
Protein pI 7.83
Protein Weight 62300.32
Protein Length 590
Protein Note Original (2000) note: Cj1120c, wlaL, pglF, possible sugar epimerase/dehydratase, len: 590 aa; 99.5% identical to TR:O86159 (EMBL: Y11648) C. jejuni 81116 wlaL (590 aa),and similar to e.g. CAPD_STAAU Staphylococcus aureus capsular polysaccharide biosynthesis CAPD protein (599 aa), fasta scores; opt: 1051 z-score: 1218.6 E(): 0, 38.4% identity in 614 aa overlap, and TR:O69130 (EMBL: AF064070) Burkholderia pseudomallei putative epimerase/dehydratase WBII (637 aa), fasta scores; opt: 961 z-score: 1113.9 E(): 0, 36.0% identity in 605 aa overlap. No Hp ortholog. Contains probable integral membrane domain at N-terminus~Updated (2006) note: Pfam domain PF02719 Polysaccharide biosynthesis protein identified within CDS. Characterised in Campylobacter jejuni. Product function modified to more specific family member. This dehydratase forms EDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hexos-4-ulose. Identifed as part of Campylobacter jejuni N-linked glycosylation locus (Cj1119c - Cj1130c). Functional classification - Surface polysaccharides,lipopolysaccharides and antigens~PMID:16421095 PMID:15752194, PMID:16186480,PMID:16618123, PMID:16547029, PMID:16286454
DNA Sequence 
>gi|15791399:1052029-1053801 Campylobacter jejuni subsp. jejuni NCTC 11168 chromosome, complete genome
ATTATACACCTTCTTTATTGTGTTTAAATTCAGGTACAATCTTTAATAAAGCCTCTGAAATATCTTCACA
TATTTGTAAATTTTCTATCTCTTTATTAAGCCAATCAAGGTCAACCTTCTCATTCTTTGCTACAAAAATA
CTCTCATATTGTGTTTTAGCATCATTTTCATCAATCAAAAGCTCTTCATAAAGCTTCTCACCTTTTCTTA
AGCCTGTGATTTTAATTTCTAAATCATTGCGATTAGAAAGTAAAAGCATTTTTTTAGCTAAATCTATGAT
TTTCACAGGCTTACCCATATCCAAAACAAAAAGTTCTCCCCCTTTTGCGATAGCTCCAGCTTGTAAAACA
AGTTGCACTGCCTCAGCCACAAGCATAAAATAACGCACTATATCAGGGTGCGTTAAAGTTAAAGGCTCAT
TATTGGCAATTTGTGCTTTAAATTTCGGTATCACACTACCACTAGAACCTAAAACATTACCAAAACGCAC
ACAAGCAACTTCAAAATTTTCATCACTCATACTTAAAGTATAAAGCTCGCAAACTCTCTTAGTGCAACCC
ATAATATTTGTTGGTCGTACTGCTTTATCTGTACTTATCATCACAAATTTAGCTACTTTGTTTTCTTTAG
CACTGTCGCATAAAATTTTAGTTCCTAAAATATTATTGATTACTGCTGAATGTGGATTTTGTTCGCAAAG
AGGCACATGTTTATAAGCGGCTGCATGTAAAATAAGCTCGGGTTTATAAGTTTTTAATACCTCATCTAAA
CTTTGCTTATCTAAAATACTCAGTAAAATAGGAGTAATTTTTTCTTTATATAAATTTAAATCATCATTGA
TCTTATAAAGATTATACTCACTATGATCAACCATGATAAGATGCTTAGCACCAAATTTAATACATTGCTT
ACAAAGTTCACTGCCTATAGTTCCACCTGCTCCACTTACCAAAACTACCTTATCTTTTAAAAAAGCCGCC
ACAGCACTATCATCTAAATCTTTTGGTTTTCTAGCAAGCAAGTCTTCTATACTGATATCTCTTGCTTCGT
TTCTTGTAAAAGAAAATATTTTTACATCGCAAATACCATAAGCTACAAGTTCTTCAAAAAGTTTTTTAAG
CTCTTCTTGTTCAAGTCTTAAAGCAATAATGGCAGTTTTTACCCCTTGTTCTACATAAGATTTTATTTTT
TCTTTTTCTTCTACAATAAATTTATCACAATAAGTCCCTATAAGCTCTTTTCTCGCATCAACTACGCCTA
CAGGAAAAAGCCCTAAAGAACCTTCTTTTGCACCTTTTAACAAATGCAAAGCCTTAGAAGTTGCCCCTAC
TACAATACAAGGAGTTTCTTCTTCTTTCATTCTAGAAGGTTTAAAATCCACAAGCATTCTTTTGCTAATT
CTTAAAGTACCTATAAACATATAAGAAAGAACAAAATCTATCACAATAGCACTTCTTGGAAAAGGATTAA
AAAAATCACTAAAAAAATAAAAAATAAGAAAAAAACAAAACTCAGCTAAAAGCAAAGCGATAAAAATCTT
TCTTGCTTCATTGAGAGAAAAAAATCTCCAAGCTACTTTATAAATTCTAAAAACAAACAAAAATGAAAGT
TTTAAAACAAGCAAAATAATAGCAGAAACCATCATACCATGATAAAAAATACTCGGAATATCTCCACTAA
ATCTCAAAGAAAAAGCCAGATAAACGCTAAGTAAAATTAAAACAATATCTGAAGTTAAAAAAAATGCTAA
TCTTTTGCTTTTATAAAAAATCA
Protein Sequence 
>gi|218562734|ref|YP_002344513.1| UDP-GlcNAc C4,6 dehydratase [Campylobacter jejuni subsp. jejuni NCTC 11168]
MIFYKSKRLAFFLTSDIVLILLSVYLAFSLRFSGDIPSIFYHGMMVSAIILLVLKLSFLFVFRIYKVAWR
FFSLNEARKIFIALLLAEFCFFLIFYFFSDFFNPFPRSAIVIDFVLSYMFIGTLRISKRMLVDFKPSRMK
EEETPCIVVGATSKALHLLKGAKEGSLGLFPVGVVDARKELIGTYCDKFIVEEKEKIKSYVEQGVKTAII
ALRLEQEELKKLFEELVAYGICDVKIFSFTRNEARDISIEDLLARKPKDLDDSAVAAFLKDKVVLVSGAG
GTIGSELCKQCIKFGAKHLIMVDHSEYNLYKINDDLNLYKEKITPILLSILDKQSLDEVLKTYKPELILH
AAAYKHVPLCEQNPHSAVINNILGTKILCDSAKENKVAKFVMISTDKAVRPTNIMGCTKRVCELYTLSMS
DENFEVACVRFGNVLGSSGSVIPKFKAQIANNEPLTLTHPDIVRYFMLVAEAVQLVLQAGAIAKGGELFV
LDMGKPVKIIDLAKKMLLLSNRNDLEIKITGLRKGEKLYEELLIDENDAKTQYESIFVAKNEKVDLDWLN
KEIENLQICEDISEALLKIVPEFKHNKEGV
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: a Cj1120c C. jejuni mutant was attenuated for colonization of the chick caeca (Hendrixson and DiRita, 2004)
References
Hendrixson and DiRita, 2004: Hendrixson DR, DiRita VJ. Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract. Molecular microbiology. 2004; 52(2); 471-484. [PubMed: 15066034].