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sepA from Shigella flexneri 5a

Victor ID 55
Gene Name sepA from Shigella flexneri 5a
Sequence Strain (Species/Organism) Shigella flexneri 5a
NCBI Gene ID 876712
NCBI Protein GI 13449013
Locus Tag pWR501_p074
Protein Accession NP_085229.1
Taxonomy ID 623
Plasmid No pWR501
Gene Starting Position 52282
Gene Ending Position 56376
Gene Strand (Orientation) -
Protein Name secreted protease
DNA Sequence 
>gi|13448939:52282-56376 Shigella flexneri 5a virulence plasmid pWR501, complete sequence
GTCAGAAAACATAACGGAAGTTGGCGTTTATCGCATTATCCACATTATATTTCCCGAACGCTGATTTCTC
CAGCTCCAGTCCCAAACGCATATTATCCTTAATTTCCGCATTCATCCCTACCGTCATCAGCATCCTGCTA
TCTTTTTCACCTTCGAAACGTTTCTCTCCGGAAGCATCCTGCAGAACCGTTTCTCCGTTCGCCAGCAGGT
CGAACTGATAACCCAGCCCGGCTCGAGCTGTGATTTTCCAGTCGTCTCCGGAGAAGGCTCTTCCCACGTC
AACACCAGTACGGCCAATCAGTGGGTTATAATCCTTGTCTTTCATGCTCAGAGCCATTCCCCGGTCTTCC
CAGCTAAAAGCTTTTCCTGATACAGAACCGTAAACCAGCTCTATCTGTGGCTCCACCCAGGACTCTTTCG
TCAGGTGGTAACGATAACCAACTTCAGCACCGGCATACCAGGAATGAGAGCTGTAGTCTTTTGTTCCCAG
TGAGGCAAAATTCGCCGTGTGCTGATTATCATGATGGAGATATTTACCAATCAGGTCAAAATAAGCTCCG
GAATTAAAGAGTGCAGAGGCATACAGACCGCCACCCACGGATTTGTTTTTTCCACTGAATGCGTGGCTGC
TTGCATTGCTGTCCGTATAGGTCAGCAATGCCCCCGTAAATAAATCCACACCGTCCAGCTCATGCTTTCT
GTCGACACCAATCTGAACGTGAGTGTAGTTATCGCTGTAGTCACCATCTGCCGAACCGGTACCATTCATT
ATGCGTGCCCAGACACCGGCATCCCCCTGAGTATCCCGCAGGTCACCCATACGTTTGTTCAGGTTATTGA
CCTCTGTAAGGAAGGACTTATAACCTGATGCCATAAAGTCTTTTGCGGCTTTCGAGGCTCCGGCATCAGC
GGTAGTCTGATATCCTGTCAGCACCCATTTTGTGGCATCATCCGTTTTTTCCGTGCTGATTACTGGCGTA
ACGTTACTGAAACCAATCTGCTGCGTACCGGCAGTGAACGTTTTCTCATCACTCCCTTTCGGTGCGGTAA
TTAACGTCACGCTCATTTCACTGGCTGGTTTGTTTAAAAAGTCAACCAGGACGCTATTATTACTGCCTGA
CAGTTTGTCTGTGACATTCAACTGGTCAGCCCGGCTGTTGTTGGTATGCATCAGGAACGTGGTATTATCT
GCAGAAAGCTCTTTTACTTGTAAAGTGGCAAAGTGTTTCCCGTCCCCCAGTTCAATTCGCGAGCCCTTAG
CCTTCAGTGCACCGGCCTCTGATTTTCCGTCAGAGTACCACAGAGCATTGATCATATTTACCGTACTCTG
TGCCCCCGTAATACTGCCTCTCAGAACAGCGTTATATCCCTTCATTAAGGAGCTGAATAACGGAGAGTCC
GTCTTCCCTGCATCAGCATCACTGTCTCCCAGATTAATGGTTGCCGCAGTGGTTCCTGCATGAATATCCG
AACTCAGATAGCCCATATTTTTAACAGAAAAGCTGGCGTTATCTTCGAGGTTAATCCCTTCTGTTGTAGA
AATTACGGGTGAGTAATACCCCTGTTTCTGCGCAGAAGGCATCCCCGTCAGGGTCAGAGAAGCATTTTCT
TTTACATCCACAGCCCCGGTGCTGAAAATACCGCTCTGAGCCGTAAGTTTTGCTCCTTTTCCGAGGGTCA
GGCTGCTGTTGACAAAGCTGCCAACACGGTCAAAAACAGCATTCTGAGAGGTCACACTCACGGTGCTATC
ATAAGCCTCAATACCACCGGTGAATCTCTCCATGATTTGCAGGGATGATTTATTTTCCAGCGTAACATTT
CCGCTGTAATCACTCTGGTCACCAACTGTGGTTGCAGTGGCACTCCCTTCAACAGAGGAGACCGCATTAC
CGGTTCCGTCACTCCTGTCAGTAAAGACACGGCTACTTCCCAGAATGACTGTACTTCCATTGGTGGCGTT
AATATCTCCCTGCATCTTGCCGTTTCTGGACAGATGGAATGTTGCCGCATCGAGTTTCAGTTTTTCCAGC
GTAAACTGACGGGTTTCCCAGTCGCTCTGGCTGGAAGAAGTCGTCGTTCCAGCATGAATAACTGGATGAC
CCTGGAATATCAGCGTGCCGGACTTCTTGGTTATCGAACCTTCTGGCAGGTTAACTGAACCATCAAGAGC
CAGTACATCCTTACCTGAGAGCTGCGGAATATTCACATCCATATTGCCGTTTAGTTGGCCGTGATACATA
TAAGGCTGGCTGCTGGCCTCAATTTTTTGTTGCTTCACAGTATCAATGGCTTTATTATGATCTTTTCCGA
CATTCTGCCAGACACTGCTGTTGTTCAGATCGGAGAAGAACGGGGAATATGAACTCGCTTTCAGGATGAA
ATATTGCTTGGTAGAACTGTCATAATATAGGTCTCCCGGCGCTCCTCTCCCCCCAAAAATACTGACAGTA
TTAACCGGTACATTGATGTCACTGGCTTTTAATGTCTGAAGGTCGAGTGTTACCGTTGATTTTTTCTCAC
TGTTATTGGTAATGATGGCCCCGTAGTCAGCCGCCTGAAGACGGGTAAAGGTCAGATTATTACCATTCAG
TTCCAGACGTCCTCCACGGTATCCCCACGAAATATTATCCGGATTAACTTGCTGTGAGTCAGAAAGCACA
ACTGTCGGACGACCGCTGGCAATACCAACAGAGCTGAAAGCCTGTACCTTTCCGTCAGCATCCGCCTGCT
GGTTGAGAATAACGGTACCGTCCCCCACCTTGAGTCCGCCTGCATTCACACCGGTCCCGTTTACTGTCAG
GGTACCTTCGCCAGTTTTATGCAGGTTATCACCGGCAACTCCGTTCACCTGCCACAGAACGTGGGTTCCT
TTATCGGTGATAATCCCGCCCCCCATCCATGTCTGGCCGTTCAGGGCGGACACCCTGTAATCGCCGGCAA
ACTGCAGATACCCAGCTCCCTGATTAACGGAATTCTGCAGAACGACCTCGCCATTATTGCCGGTGAACAG
GAGGTTTTTTCCTGCGTTCAGATCATTTCCTTTCTTTCCATGCATATCCCAAGTCGTGTTTCCCTGAGTC
AGTGTGCCGGTGCCATTAGCCGCATCATATTTCCACTGCAGTACTCCTTCACCAGAGGTGTAGGCTATAG
TTTTGTCAAAATCATTTTGCGGTTGCTGACCAAGGAAATCCTGAGTGGTGACGACCCAGTTATTTCCATA
GTTAGTTCCTGAGGAAAGCACGCCAATCAGTACCCATTTTTTTTGCAGGGAATCATAAGCAAACAGGGGG
GAGCCGCTGTCCCCCATATTCAGATAATTAGCCAGAGGTCCATTCGCGGGATTAAAAATGTCACCGGTCT
GAGCTGTAATCATCTGTCCGTTGTTATAGGAGTTCAGCGCGCTGACGGTTCCCCCAACAAGAAATCCGCC
ATTGGTATACACTTGGGTGCGATTACCATTTTTGTCTTTGACGTACTGCATCCCCCCCCCAAGGCGATAG
AATTCAGTAAAGCGTCCGCCGGCCTGATAGGCACCGCTCACAGCCCCGATGTCAGACACTTCTGCCGGCG
CCACCTCTGTCACCAGTTTACTCAGGCGGCGGGTTTTAATATCAAGACCGCTATTATTATTTGTACCAAC
CGCAGTATAGGTGTTTTTGGCGTATCCGAAGCTCATCGTGTCAGAACCGCTGACATGTTTGGCGGTAACC
ACATACTGAGGGGAGTAAAGTGTATGGTCTCCCGGAGGCAGGCTACCGGTAGTAATAGTGGCACTACTGA
AATCAGCCATCGGGGCTTTATCAAGTTTACCGACCAGATTGCCCTGCTTGTCGTAAATGGAAATATTCGT
GGTCCCCGGCGTAAACTGACCTTTATTCTCGGCAAAGTCGCGGAATATCTGATAAGGGATCTCTGCACTG
ACCGTTGCTGACAGAGCTGGCCATGCCGAAGAAAGTGATAAAGCTGCAACAGACGTAAGAATAACCCGAC
GTGAAAGTCTGCGATGGCTTTTGCAGGTAACCCTGCGGGCCAGTTCGGAGACAGCAATCAGGCTTTTGGT
TATATGGCAATACTTAAGATAATATATTTTGTTCA
Protein Sequence 
>gi|13449013|ref|NP_085229.1| secreted protease [Shigella flexneri 5a str. M90T] MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVAALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAPMADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSGLDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQVYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDSLQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAANGTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRVSALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDGTVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNLTFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYDSSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDHNKAIDTVKQQKIEASSQPYMYHGQLNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSDWETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVSSVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRVGSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTEGINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAVLRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNTTFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAGTQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNNLNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGALLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLGTKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKDYNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDSRMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: sepA mutant has attenuated virulence in ligated rabbit ileal loop model (Benjelloun-Touimi et al., 1995).
References
Benjelloun-Touimi et al., 1995: Benjelloun-Touimi Z, Sansonetti PJ, Parsot C. SepA, the major extracellular protein of Shigella flexneri: autonomous secretion and involvement in tissue invasion. Molecular microbiology. 1995; 17(1); 123-135. [PubMed: 7476198].