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pgaC |
| Victor ID |
5233 |
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Gene Name |
pgaC |
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NCBI Nucleotide GI |
259020301
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NCBI Protein GI |
259020302
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Locus Tag |
FJ866499 |
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Protein Accession |
ACV90435.1 |
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Other Database IDs |
CDD:236882
CDD:133045 |
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Taxonomy ID |
470
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Gene Strand (Orientation) |
? |
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Protein Name |
PgaC |
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Protein Length |
392 |
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Protein Note |
N-glycosyltransferase; Provisional |
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DNA Sequence |
>gi|259020301|gb|FJ866499.1| Acinetobacter baumannii strain S1 PgaC (pgaC) gene, complete cds
ATGGTAGGAGGTCTCTGGTTTTTCTTTAAAAGGGAATATCATGAGCAGCAACTGCCTGAGCCATCAAGTG
AAGGGTGCAGTATTATTATTCCTTGCTTTAATGAAGAAGCTCAGGTCCGACAAACGATTCGTTATGCATT
ACAAACAAAATATCCGAATTTTGAAGTCATTGCCGTAAATGACGGAAGTAGTGACAGCACTGCAGAAATT
CTTGATGAACTGGCAGCTCAAGATGCCCGATTAAGAGTTGTGCACCTGGCAGAAAACCAAGGTAAGGCCG
TAGCTTTAAGATCGGGAGTGTTAGTAAGTAAATACGAATATCTAGTTTGTATTGACGGAGACGCTTTATT
ACATCCTCATGCAGTACTTTGGCTTATGCAGCCTTTTTTAAATTTTCCAAGAATTGGTGCTGTAACCGGT
AATCCTAGAATTTTAAATCGCTCAAGTATTTTAGGTAAATTACAAGTTGGCGAGTTCTCTTCAATTATTG
GCTTGATTAAGCGCGCACAAAGAACATATGGCCGTATTTTTACAGTATCAGGTGTAATTGCAGCATTTAG
AAAGACCGCTTTGGTTCGCGTGGGCTTTTGGTCTGATGACAAAATTACGGAAGATATTGATATCTCTTGG
AAGCTCCAAATGGACCATTGGGACATTCAATATATTCCTCAAGCACTTTGTTATATCTATATGCCGGAAA
CCTTTAAAGGCCTTTGGAAACAGCGTTTACGCTGGGCACAAGGTGGGGTAGAGGTATTATTAGAATATAT
TCCGAAAATGTTCAAACTCCGCTTGCGCCGTATGTGGCCGGTAATGCTCGAAGCTTTAATCAGTATTATT
TGGTCATATGTGATGATTATGATTTTTATTCTATTTTTTGTGGGATTGTTTGTGGATTTACCGCAACAAT
TTCAAATTAATTCACTCATGCCGCAGTGGTATGGCGTGATATTAGGTGGAACATGTCTTGTCCAATTTCT
AGTAAGTTTATGGATAGACCATCGTTATGATCGTGGTCGGCTTTTTAGAAATTATTTTTGGGTAATTTGG
TATCCATTATTTTTCTGGTTACTGACCTTATTTACCAGTGTTGTTGCAGTACCCAAAACTATATTTAACA
CCAAAAAACGTGCTCGTTGGGTAAGCCCTGACCGAGGCTTCCGTGGAGATCATTCATGA
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Protein Sequence |
>gi|259020302|gb|ACV90435.1| PgaC [Acinetobacter baumannii]
MVGGLWFFFKREYHEQQLPEPSSEGCSIIIPCFNEEAQVRQTIRYALQTKYPNFEVIAVNDGSSDSTAEI
LDELAAQDARLRVVHLAENQGKAVALRSGVLVSKYEYLVCIDGDALLHPHAVLWLMQPFLNFPRIGAVTG
NPRILNRSSILGKLQVGEFSSIIGLIKRAQRTYGRIFTVSGVIAAFRKTALVRVGFWSDDKITEDIDISW
KLQMDHWDIQYIPQALCYIYMPETFKGLWKQRLRWAQGGVEVLLEYIPKMFKLRLRRMWPVMLEALISII
WSYVMIMIFILFFVGLFVDLPQQFQINSLMPQWYGVILGGTCLVQFLVSLWIDHRYDRGRLFRNYFWVIW
YPLFFWLLTLFTSVVAVPKTIFNTKKRARWVSPDRGFRGDHS
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
Deletion of the pga locus (the pgaABC genes) led to loss of the strong biofilm phenotype, which was restored by complementation (Choi et al., 2009).
pgaC is predicted to encode a 392-amino-acid N-glycosyltransferase that belongs to the glycosyltransferase 2 family. This family includes PgaC, BpsC, HmsR, and IcaA from E. coli, B. pertussis, Y. pestis, and S. aureus, respectively. A. baumannii PgaC shares 55% identity with E. coli PgaC, 47% identity with B. pertussis BpsC, and 55% identity with Y. pestis HmsR. In addition, the five amino acids that have been shown to be critical for the catalytic activity of glycosyltransferases are all conserved in PgaC (Asp112, Asp205, Gln241, Arg244, and Trp245) (Choi et al., 2009). |
| References |
Choi et al., 2009: Choi AH, Slamti L, Avci FY, Pier GB, Maira-Litrán T. The pgaABCD locus of Acinetobacter baumannii encodes the production of poly-beta-1-6-N-acetylglucosamine, which is critical for biofilm formation. Journal of bacteriology. 2009; 191(19); 5953-5963. [PubMed: 19633088].
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