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18K

Victor ID 5131
Gene Name 18K
NCBI Nucleotide GI 557798815
NCBI Protein GI 557798816
Protein Accession AHA36956.1
Other Database IDs CDD:151579
Taxonomy ID 1419340
Gene Strand (Orientation) ?
Protein Name 18K immediate early protein
Protein Length 157
Protein Note Protein of unknown function (DUF2888); pfam11135
DNA Sequence 
>gi|557798815|gb|KF703533.1| Zoo ranavirus 18K immediate early protein gene, complete cds
ATGGAGATTGAAACTATTTTACGGGACAATCGCCTCACTCTAAAACACTTTACATTCGCCATGAGCATGA
TCCAAGCCTACCTGTGTGACTCTGTGTCTGGAGAACCCTACACTTGCAAGGGGGACCTGTGCGAGATCCC
CTTTAACAAAAACTTTACCATAGATTTGGTAAACCTGTCTGTCTCTACAGAGTTTCAGGTCAGGATCACC
ATAACTCCCCACCACGACCTGGGAACCTTTGTGGTGGAGCCCAAGAAAGTCTTTTCCATCAAGAGGGCCA
CAAAGGGAGACGCGGCCTTTAAGGTGGAAAGGGCCGCCGGGTGGCTGCCCGACACTCCCCAAGTCCTGTC
CCTCTTTGTCTACAAGAGGCTCCATCCCGTCGAATGGCACAGCGAATGCCTGTACGAAAACCTGGAGACT
GACGGGGGCACGGTCATCGTCCCCGGCGAGGCCACCGGGCAGAGGTTCGGGACTGCGACAGCGGTCCCCA
CCAGGTTTCTCTTTAAGAGAATGTTTGTCGTAAAGGGTGTATAG
Protein Sequence 
>gi|557798816|gb|AHA36956.1| 18K immediate early protein [Zoo ranavirus]
MSMIQAYLCDSVSGEPYTCKGDLCEIPFNKNFTIDLVNLSVSTEFQVRITITPHHDLGTFVVEPKKVFSI
KRATKGDAAFKVERAAGWLPDTPQVLSLFVYKRLHPVEWHSECLYENLETDGGTVIVPGEATGQRFGTAT
AVPTRFLFKRMFVVKGV
Molecule Role Virulence factor
Molecule Role Annotation By successive rounds of selection for puromycin resistance and GFP expression, we have successfully constructed three recombinant viruses. In one, a "knock-in" mutant was created by inserting the Puro-EGFP cassette into a noncoding region of the FV3 genome (FV3-Puro/GFP). In the remaining two, KO mutants were constructed by replacement of the truncated viral homolog of eIF-2α (FV3-ÃŽâ€vIF-2α) or the 18K IE gene (FV3-ÃŽâ€18K) with the Puro-EGFP cassette Viral replication of each recombinant in cell culture was similar to that of parental FV3; however, infection in Xenopus laevis tadpoles revealed that FV3-ÃŽâ€vIF-2α and FV3-ÃŽâ€18K replicated less and resulted in lower mortality than did GFP-FV3 and wild-type FV3. Our results suggest that 18K, which is conserved in all ranaviruses, and the truncated vIF-2α gene contribute to virulence. (Chen et al., 2011)
References
Chen et al., 2011: Chen G, Ward BM, Yu KH, Chinchar VG, Robert J. Improved knockout methodology reveals that frog virus 3 mutants lacking either the 18K immediate-early gene or the truncated vIF-2alpha gene are defective for replication and growth in vivo. Journal of virology. 2011; 85(21); 11131-11138. [PubMed: 21865381].