Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

ORF2

Victor ID 5120
Gene Name ORF2
Sequence Strain (Species/Organism) Porcine circovirus 2
NCBI Gene ID 2943185
NCBI Protein GI 38018062
Locus Tag PCV2_gp3
Genbank Accession AY424401
Protein Accession NP_937957
Taxonomy ID 85708
Gene Starting Position 1033
Gene Ending Position 1734
Gene Strand (Orientation) -
Protein Name capsid protein
Protein pI 11.09
Protein Weight 26364.11
Protein Length 233
DNA Sequence 
>gi|38018060:1033-1734 Porcine circovirus 2, complete genome
TTTAGGGTTTAAGTGGGGGGTCTTTAAGATTAAATTCTCTGAATTGTACATACATGGTTACACGGATATT
GTAGTCCTGGTCGTATTTACTGTTTTCGAACGCAGTGCCGAGGCCTACGTGGTCCACATTTCTACTGGTT
TGTAGTCTCATCCACAGCTGATTCCTTTTGTTATTTGGTTGGAAGTAATCAATAGTGGAATCAAGAACAG
GTTTGGGTGTGAAGTAACGGGAGTGGTAGGAGAAGGGTTGGGGGATTGTATGGCGGGAGGAGTAGTTTAC
ATAGGGGTCATAGGTTTGGGCTGTGGCCTTTATTACAAAGTTGTCATCTAGAATAATAGCACTGGATCCA
ACTCCCCTGTCACCCTGGGTGATCGGGGAGCAGGGCCAGAATTCAACCTTAACCTTTCTTATTCTGTAGT
ATTCAAAGGGTATAGAGATTTTGTTGGTCCCCCCTCCCGGGGGAACAAAGTCGTCAAGATTAAATCTCAG
CATGTCCACCGCCCAGGAGGGCGTGCTGACTGTGGTAGCCTTGACAGTATATCCGAAGGTGCGGGAGAGG
CGGGCGTTGAAGATGCCATTTTTCCTTCTCCAGCGGTAACGGTGGCGGGGGTGGACGAGCCAGGGGCGGC
GGCGGAGGATCTGGCCAAGATGGCTGCGGGGGCGGTGTCTTCTCCTTCGGTAACGCCTCCTTGGATACGT
CA
Protein Sequence 
>gi|38018062|ref|NP_937957.1| capsid protein [Porcine circovirus-2]
MTYPRRRYRRRRHRPRSHLGQILRRRPWLVHPRHRYRWRRKNGIFNARLSRTFGYTVKATTVSTPSWAVD
MLRFNLDDFVPPGGGTNKISIPFEYYRIRKVKVEFWPCSPITQGDRGVGSSAIILDDNFVIKATAQTYDP
YVNYSSRHTIPQPFSYHSRYFTPKPVLDSTIDYFQPNNKRNQLWMRLQTSRNVDHVGLGTAFENSKYDQD
YNIRVTMYVQFREFNLKDPPLKP
Molecule Role Virulence factor
Molecule Role Annotation After 120 passages, only two nucleotide mutations were identified in the entire genome, and both were located in the capsid gene: the mutations were located at nucleotide positions 328 (C328G) and 573 (A573C). The PCV2 genomic copy numbers in serum in the PCV2 VP1 group were significantly higher than those in the PCV2 VP120 group (P < 0.0001). Gross and histopathologic lesions in pigs inoculated with PCV2 VP1 were more severe than those inoculated with PCV2 VP120 at both day 21 and 42 necropsies (P = 0.0032 and P = 0.0274, respectively). Taken together, the results from this study indicated that the P110A and R191S mutations in the capsid of PCV2 enhanced the growth ability of PCV2 in vitro and attenuated the virus in vivo. This finding has important implications for PCV2 vaccine development. (Fenaux et al., 2004)
References
Fenaux et al., 2004: Fenaux M, Opriessnig T, Halbur PG, Elvinger F, Meng XJ. Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo. Journal of virology. 2004; 78(24); 13440-13446. [PubMed: 15564454].