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EVM027

Victor ID 5099
Gene Name EVM027
Sequence Strain (Species/Organism) Ectromelia virus
NCBI Gene ID 951567
NCBI Protein GI 22164632
Locus Tag ECTVgp027
Genbank Accession AF012825
Protein Accession NP_671545
Taxonomy ID 12643
Gene Starting Position 40259
Gene Ending Position 41707
Gene Strand (Orientation) -
Protein Name Kelch-like protein
Protein pI 8.1
Protein Weight 52956.42
Protein Length 482
Protein Note Kelch-like protein; intracellular protein expressed in late infection stages; may be involved in remodelling and stabilization of the host cytoskeleton; contains a BTB/POZ domain which mediates dimerisation; similar to Vaccinia virus Copenhagen F3L; the poxviridae are enveloped unsegmented dsDNA viruses; unlike many dsDNA viruses that replicate in the host nucleus poxviruses encode their own replication machinery and therefore replicate in the cytoplasm; viral genes are expressed in a bi-phasic manner with early genes genes encoding non-structural proteins involved in genome replication and late genes encoding the viral structural proteins
DNA Sequence 
>gi|22164589:40259-41707 Ectromelia virus, complete genome
TTTACCATCTTATCCCATTCCATATATTCCATGAATAAGTGCGATTATTGTACACTTCTATAGTATCTAT
ATACGATCCACGATAAAATCCTCCTATCAATAGCAGTTTATTATCCACTATGATCAATTCTGGATTATCC
CTCGGATAAATAGGATCATCTATCAGAGTCCATGTATTGCTGGATCCACAATAAAATTCAGCATTTCTAC
CAACCAAGAATAACCTTCTACCGAACACTAACGCGCATGATTTATAATGAGGATAATAAGTTGATGGTCC
AAACTGCCACTGATTATGATTGGGTAGCAAATATTCTGTAGTTGTATCCGTTTCAGAATGTCCTCCTATT
ACGTATATAACATTGTTTATAGACGCGATCGCTGGATTATATCTAGGTTTCAGAAGACTCGGCATATTAA
CCCAAGAAGTATCTCCGTGGAACCAACACTCGACAGATGTGGGATTTGGTAGACCTCCTACTACGTATAA
TTTATTGTTAGCAGCTACCCCGCTAGCATACAGTCTGGGACTATTCATCGGAGGAATTGGAATCCAATTG
TTTGATGTACAATTTAACGCTATAGCATTGTTATGTATTTCATTGTTCATCCATCCACCGATGAGATATA
CTACTTCTCCAACATGAGTACTTGTACACATATGGAATATATCTATAATTTGATCCATGTTCATATGATA
CTCTATGAATGGATACTTGTATGATTTGCGTGGTTGTTTATCACAATGAAATATTTTGGTACAGTCTAGT
ATCCATTTTACATTATTTATACCTCTGGGAGAAAGATAATTTGACCTGATTACATTTTTGATAAGGAGTA
GCAAATTTCCTAATCGATTTCTTCGCTTCATATACCACTTAATGACAAAATCAACTACATAATCCTCATC
TGGAACATTTAGTTCATTGCTTTCTAGAATAAGTTTCATAGATAGATAATCAAAATTGTCTATGATGTCA
TCTTCCAGTTCCAAAAAGTGTTTGGTAATAAAGTCTTTAGTATGACATAAGAGATTGGATAGTCCGTATT
CTATACCCATCATGTAACACTCGACACAATATTCCTTTCTAAAATCTCGTATGATAAAGTTTATACAAGT
GTAGATGATAAATTCTACAGAGGTTAATATAGAAGCACGTAATAAATTGACAACGTTATGACTATCTATA
TATACCTTTCCAGTATATGAGTAAATAACTATAGAAGTTAGGCTGTGAATGTCAAGGTCTAGACAAACCC
TTGTAACTGGATCTTTATTTTTTGTGTATTTTTGACGTAAATGTGTGCGAAAGTAAGGAGACAACTTTTT
CAATATCGTAGAATTGACTATTATATTGCCTCCTATAGCCTCAATAATTGTTTTGAATTTCTTAGTCATA
GACAATGCTAATATATTCTTACAGTACACAGTATTGACAAATATCGGCA
Protein Sequence 
>gi|22164632|ref|NP_671545.1| EVM027 [Ectromelia virus]
MPIFVNTVYCKNILALSMTKKFKTIIEAIGGNIIVNSTILKKLSPYFRTHLRQKYTKNKDPVTRVCLDLD
IHSLTSIVIYSYTGKVYIDSHNVVNLLRASILTSVEFIIYTCINFIIRDFRKEYCVECYMMGIEYGLSNL
LCHTKDFITKHFLELEDDIIDNFDYLSMKLILESNELNVPDEDYVVDFVIKWYMKRRNRLGNLLLLIKNV
IRSNYLSPRGINNVKWILDCTKIFHCDKQPRKSYKYPFIEYHMNMDQIIDIFHMCTSTHVGEVVYLIGGW
MNNEIHNNAIALNCTSNNWIPIPPMNSPRLYASGVAANNKLYVVGGLPNPTSVECWFHGDTSWVNMPSLL
KPRYNPAIASINNVIYVIGGHSETDTTTEYLLPNHNQWQFGPSTYYPHYKSCALVFGRRLFLVGRNAEFY
CGSSNTWTLIDDPIYPRDNPELIIVDNKLLLIGGFYRGSYIDTIEVYNNRTYSWNIWNGIRW
Molecule Role Virulence factor
Molecule Role Annotation deletions of one to four genes of kelch family both individually (single mutants) and in different combinations (double, triple and quadruple mutants). It was shown that deletion of any of the three genes EVMO18, EVM027 or EVM167 resulted in reduction of 50% lethal dose (LD50) by five and more orders in outbred white mice infected intraperitoneally. Deletion of mousepox kelch-gene EVM150 did not influence the virus virulence. Two or more kelch-genes deletion also resulted in high level of attenuation, which could evidently be due to the lack of three genes EVM167, EVM018 and/or EVM027 identified as virulence factors. (Kochneva et al., 2009)
References
Kochneva et al., 2009: Kochneva GV, Kolosova IV, Lupan TA, Sivolobova GF, Iudin PV, Grazhdantseva AA, Riabchikova EI, Kandrina NIu, Shchelkunov SN. [Orthopoxvirus genes for kelch-like proteins. III. Construction of mousepox (ectromelia) virus variants with targeted gene deletions]. Molekuliarnaia biologiia. 2009; 43(4); 616-622. [PubMed: 19807023].