Victors

EVM018
Victor ID	5098
Gene Name	EVM018
Sequence Strain (Species/Organism)	Ectromelia virus
NCBI Gene ID	951501
NCBI Protein GI	22164625
Locus Tag	ECTVgp018
Genbank Accession	AF012825
Protein Accession	NP_671536
Taxonomy ID	12643
Gene Starting Position	27619
Gene Ending Position	29157
Gene Strand (Orientation)	-
Protein Name	kelch-like protein
Protein pI	8.64
Protein Weight	55166.73
Protein Length	512
Protein Note	Kelch-like protein; Kelch motifs are 50-amino acid motifs represented by one beta-sheet blade; may be involved in remodelling and stabilization of the host cytoskeleton and host immune evasion; BTB-Kelch proteins have Kelch repeats that can interact with actin filaments; the poxviridae are enveloped unsegmented dsDNA viruses; unlike many dsDNA viruses that replicate in the host nucleus poxviruses encode their own replication machinery and therefore replicate in the cytoplasm; viral genes are expressed in a bi-phasic manner with early genes genes encoding non-structural proteins involved in genome replication and late genes encoding the viral structural proteins
DNA Sequence	>gi\|22164589:27619-29157 Ectromelia virus, complete genome GCTATTGTAGGAATTTTTTTTCACAGTTGCTCAAAAACGATGGTAGTGACTTATGAGTTACATTACATTT TGGAGTCTCATCTTTAGTAAACATATCATAATATTCGATATTACGAGTTGACGTATCTAACAAATTCCAA GTATTTGATTTTGGATAATATTCGTATTTTGCATCTGCTATAATTAAGATATAATCACCACAAGAACACA CGAACATCTTTCCTACATGGTTAAAGTACATGTATAATTCTATCCATTTGTCTTCATTAACTATATATTT GTATAGATAATTACGAGTCTCATGAGTAATTCCAGTAATTGCATAGATGTCACCATCGTACTCTACAGCA TAAACTATACTATGATGTCTAGGCATGGGAGACTTTTTTATCCAACGATTTTTAGTGAAACATTCCACAT CATTTAATACTACATATTTCTCATACGTGGTATAAACTCCACCCATTACATATATATCATCGTTTACGAA TACCGATGCTCCTGAATATCTAGGAGTAATTAAGTTTGGAAGTCTTATCCATTTCGAAGCGCCATGTTTC AAATATTCTGCTACAGCCGTTGAAATAGAAAATTCTAATCCTCCTATTACATATAACTTTCCATTGTTAA CACAAGTACTAACTTCTGATTTTAACGACGACATATTAGTGACCGTTTTCCATTTTTTCGTTTTAAGATC TACCCGCGATACGGAATAAACATGTCTATTGTTAATCATGCCGCCAATAATGTATAGACAATTATGTAAA ACATTTGCATTATAGAATTGTCTATCTGTATTACCGACTATCGTCCAATATTCTGTTCTAGGAGAGTAAT GGGTTATTGTGGATATATAATCAGAGTTTTTAATGACTACTATATTATGTTTTATACCATTTCGTGTCAC AGGCTTTGTAGATTTGGATATAGTTAATCCCAACAATGCTATAGCATTGCGCATAGCATTAGTCATAAAC TTGGGATGTAAAATGTTGATGATATCTACATCGTTTGGATTTTTATGTATCCACTTTAATAATATTATAG CTGTAACATCCTCATGATTTACGTTAACGTTTTCGTGGGATAAGATAGTTGTTAGTTCATCCTTTGATAA TTTTCCAAATTCTGGATCGGATGTCACCGCTGTAATATTGTTGATTATTTCTGACATCGACGCATTATAT AGTTTTTTAATTCCATATCTTTTAGAAAAGTTAAACATCCTTATACAATTTGTGGAATTAATATTATGAA TCATAGTTTTTACACATAGATCTACTACAGGCGGAACATCAATTATTATGGCAGCAACTAGTATCATTTC TACATTGTTTATGGTGATGTTTATCTTCTTCCAGCGCATATAGTCTAATAGCGATTCAAACGCGTGATAG TTTATACCATTCAATATAATCGCTTCATCCTTTAGATGGTGATCCTGAATGCGTTTAAACAAATTATACG GAGACGCCGTAATAATTTCCTTATTCACTTGTATAATTTCCCCATTGATAGAAAATATCACGCTTTCCA
Protein Sequence	>gi\|22164625\|ref\|NP_671536.1\| EVM018 [Ectromelia virus] MESVIFSINGEIIQVNKEIITASPYNLFKRIQDHHLKDEAIILNGINYHAFESLLDYMRWKKINITINNV EMILVAAIIIDVPPVVDLCVKTMIHNINSTNCIRMFNFSKRYGIKKLYNASMSEIINNITAVTSDPEFGK LSKDELTTILSHENVNVNHEDVTAIILLKWIHKNPNDVDIINILHPKFMTNAMRNAIALLGLTISKSTKP VTRNGIKHNIVVIKNSDYISTITHYSPRTEYWTIVGNTDRQFYNANVLHNCLYIIGGMINNRHVYSVSRV DLKTKKWKTVTNMSSLKSEVSTCVNNGKLYVIGGLEFSISTAVAEYLKHGASKWIRLPNLITPRYSGASV FVNDDIYVMGGVYTTYEKYVVLNDVECFTKNRWIKKSPMPRHHSIVYAVEYDGDIYAITGITHETRNYLY KYIVNEDKWIELYMYFNHVGKMFVCSCGDYILIIADAKYEYYPKSNTWNLLDTSTRNIEYYDMFTKDETP KCNVTHKSLPSFLSNCEKKFLQ
Molecule Role	Virulence factor
Molecule Role Annotation	deletions of one to four genes of kelch family both individually (single mutants) and in different combinations (double, triple and quadruple mutants). It was shown that deletion of any of the three genes EVMO18, EVM027 or EVM167 resulted in reduction of 50% lethal dose (LD50) by five and more orders in outbred white mice infected intraperitoneally. Deletion of mousepox kelch-gene EVM150 did not influence the virus virulence. Two or more kelch-genes deletion also resulted in high level of attenuation, which could evidently be due to the lack of three genes EVM167, EVM018 and/or EVM027 identified as virulence factors. (Kochneva et al., 2009)
References	*Kochneva et al., 2009:* Kochneva GV, Kolosova IV, Lupan TA, Sivolobova GF, Iudin PV, Grazhdantseva AA, Riabchikova EI, Kandrina NIu, Shchelkunov SN. [Orthopoxvirus genes for kelch-like proteins. III. Construction of mousepox (ectromelia) virus variants with targeted gene deletions]. Molekuliarnaia biologiia. 2009; 43(4); 616-622. [PubMed: 19807023].


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EVM018