>gi|27885149|ref|NP_777500.1| nonstructural protein p7 [Classical swine fever virus]
LPLGQGEVVLIGNLITHTDIEVVVYFLLLYLVMRDEPIKKWILLLFHAMTNNPVKTITVALLMVSGVAKG
Molecule Role
Virulence factor
Molecule Role Annotation
Using reverse genetics, partial in-frame deletions of p7 were deleterious for virus growth, demonstrating that CSFV p7 function is critical for virus production in cell cultures. A panel of recombinant mutant CSFVs was created using alanine scanning mutagenesis of the p7 gene harboring sequential three- to six-amino-acid residue substitutions spanning the entire protein. These recombinant viruses allowed the identification of the regions within p7 that are critical for virus production in vitro. In vivo, some of these viruses were partially or completely attenuated in swine relative to the highly virulent parental CSFV Brescia strain, indicating a significant role of p7 in CSFV virulence (Gladue et al., 2012).
References
Gladue et al., 2012: Gladue DP, Holinka LG, Largo E, Fernandez Sainz I, Carrillo C, ODonnell V, Baker-Branstetter R, Lu Z, Ambroggio X, Risatti GR, Nieva JL, Borca MV. Classical swine fever virus p7 protein is a viroporin involved in virulence in swine. Journal of virology. 2012; 86(12); 6778-6791. [PubMed: 22496228].
