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nsP1

Victor ID 5087
Gene Name nsP1
NCBI Protein GI 25121492
Other Database IDs CDD:250778
Taxonomy ID 11029
Gene Strand (Orientation) ?
Protein Name nsP1 protein
Protein Length 533
Protein Note Viral methyltransferase; pfam01660
Protein Sequence 
>gi|25121492|ref|NP_740678.1| nsP1 protein [Ross River virus]
MKVTVDVEADSPFLKALQKAFPAFEVESQQVTPNDHANARAFSHLATKLIEQEVPANITILDVGSAPARR
LMSDHSYHCICPMKSAEDPERLANYARKLAKTAGEVLDKNVSGKITDLQDVMATPDLESPTFCLHTDETC
RTRAEVAVYQDVXXHAPTSLYHQAMKGVRTVYWIGFDTTPFMFEVVAGAYPTYSTNWADEQVLQARNIGL
CATSLSEGHRGKISIMRKKRLRPSDRXMFSVGXTLYIESRRLLKSWHLPSVFHLKGKNSFTCRCDTIVSC
EGYVVKKITMSPGTYGKTVGYAVTHHAEGFLMCKVTDTVRGERVSFPVCTYVPATICDQMTGILATDVTP
EDAQKLLVGLNQRIVVNGRTQRNTNTMKNYLLPVVAQAFSKWAREAKADMEDEKPLGTRERTLTCCCLWA
FKNHKTHTMYKRPDTQTIVKVPSTFDSFVIPSLWSSSLSIGIRQRIKLLLGPKLSRDLPYSGDRNEAREA
EKEAEETKEAELTREALPPLVGSNCADDVDQVDVEELTYRAGA
Molecule Role Virulence factor
Molecule Role Annotation we identify two nonsynonymous nucleotide changes as sufficient for the attenuated phenotype. RRV T48 carrying the six nonsynonymous DC5692 nucleotide differences (RRV-T48-nsP1(6M)) was attenuated in both wild-type and Rag1(-/-) mice. Despite the attenuated phenotype, RRV T48 and RRV-T48-nsP1(6M) loads in tissues of wild-type and Rag1(-/-) mice were indistinguishable from 1 to 3 days postinoculation. RRV-T48-nsP1(6M) loads in skeletal muscle tissue, but not in other tissues, decreased dramatically by 5 days postinoculation in both wild-type and Rag1(-/-) mice, suggesting that the RRV-T48-nsP1(6M) mutant is more sensitive to innate antiviral effectors than RRV T48 in a tissue-specific manner.
(Stoermer et al., 2014)
References
Stoermer et al., 2014: Stoermer Burrack KA, Hawman DW, Jupille HJ, Oko L, Minor M, Shives KD, Gunn BM, Long KM, Morrison TE. Attenuating mutations in nsP1 reveal tissue-specific mechanisms for control of Ross River virus infection. Journal of virology. 2014; 88(7); 3719-3732. [PubMed: 24429363].