Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

N2L

Victor ID 5051
Gene Name N2L
Sequence Strain (Species/Organism) Vaccinia virus
NCBI Gene ID 3707644
NCBI Protein GI 66275826
Locus Tag VACWR029
Genbank Accession M22812
Protein Accession YP_232911
Taxonomy ID 10245
Gene Starting Position 22308
Gene Ending Position 22835
Gene Strand (Orientation) -
Protein Name putative alpha aminitin-sensitive protein
Protein pI 7.49
Protein Weight 19661.19
Protein Length 175
Protein Note putative alpha aminitin-sensitive protein; mutations in this protein may confer resistance to alpha-amanitin, an inhibitor of RNA polymerase II; similar to vaccinia virus strain copenhagen N2L and lumpy skin disease virus 009; The poxviridae are enveloped unsegmented dsDNA viruses; unlike many dsDNA viruses that replicate in the host nucleus poxviruses encode their own replication machinery and therefore replicate in the cytoplasm; viral genes are expressed in a bi-phasic manner with early genes encoding non-structural proteins involved in genome replication and late genes encoding the viral structural proteins
DNA Sequence 
>gi|66275797:22308-22835 Vaccinia virus, complete genome
TTTAGAAATACTTAGTTTCCACGTAGTTAATGAAACATTTGAACTCATCGTACAGGACGTTCTCGTACAG
GACGTAACTATAAACCGGTTTATATTTGTTCAAGATAGATACAAATCCGATAACTTTTTTTACGAATTCT
ACGGGATCCACTTTAAAAGTGTCATACCGGGTTCTTTTTATTCTTTTAAACAGATCAATGGTGTGATGTT
GATTAGGTCTTTTACGAATTTGATATAGAATAGCGTTCACATATCCTCCATAATGGTCAATCGCCATTTG
TTCGTATGTCATAAATTCTTTAATTATATGACACTGTGTATTATTTAGTTCATCCTTGTTCATCATTAGG
AATCTATCCAAAATGGCAATTATACTAGAACTATAGGTGCGTTGTATACACATATTGATGTGTCTGTTTA
TACAATCCATGATATTTGGATCCATGCTACTACCTTCGGGTAAAATTGTAGCATCATATACCATTTCTAG
TACTTTAGGTTCATTATTATCCATTGCAGAGGACGTCA
Protein Sequence 
>gi|66275826|ref|YP_232911.1| alpha-amanitin target [Vaccinia virus]
MTSSAMDNNEPKVLEMVYDATILPEGSSMDPNIMDCINRHINMCIQRTYSSSIIAILDRFLMMNKDELNN
TQCHIIKEFMTYEQMAIDHYGGYVNAILYQIRKRPNQHHTIDLFKRIKRTRYDTFKVDPVEFVKKVIGFV
SILNKYKPVYSYVLYENVLYDEFKCFINYVETKYF
Molecule Role Virulence factor
Molecule Role Annotation A mutant VACV strain Western Reserve lacking the N2L gene (vÃâN2) showed normal replication and spread in cultured cells compared to wild-type parental (vN2) and revertant (vN2-rev) viruses, but was attenuated in two murine models of infection. After intranasal infection, the vÃâN2 mutant induced lower weight loss and signs of illness, and virus was cleared more rapidly from the infected tissue. In the intradermal model of infection, vÃâN2 induced smaller lesions that were resolved more rapidly. In summary, the N2 protein is an intracellular virulence factor that inhibits IRF3 activity in the nucleus. (Ferguson et al., 2013)
References
Ferguson et al., 2013: Ferguson BJ, Benfield CT, Ren H, Lee VH, Frazer GL, Strnadova P, Sumner RP, Smith GL. Vaccinia virus protein N2 is a nuclear IRF3 inhibitor that promotes virulence. The Journal of general virology. 2013; 94(Pt 9); 2070-2081. [PubMed: 23761407].