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J2R

Victor ID 5048
Gene Name J2R
Sequence Strain (Species/Organism) Vaccinia virus
NCBI Gene ID 3707550
NCBI Protein GI 66275891
Locus Tag VACWR094
Genbank Accession JN654986
Protein Accession YP_232976
Taxonomy ID 10245
Gene Starting Position 80723
Gene Ending Position 81256
Gene Strand (Orientation) +
Protein Name thymidine kinase
Protein pI 5.83
Protein Weight 19008.31
Protein Length 177
Protein Note similar to VACCP-J2R
DNA Sequence 
>gi|66275797:80723-81256 Vaccinia virus, complete genome
CATGAACGGCGGACATATTCAGTTGATAATCGGCCCCATGTTTTCAGGTAAAAGTACAGAATTAATTAGA
CGAGTTAGACGTTATCAAATAGCTCAATATAAATGCGTGACTATAAAATATTCTAACGATAATAGATACG
GAACGGGACTATGGACGCATGATAAGAATAATTTTGAAGCATTGGAAGCAACTAAACTATGTGATGTCTT
GGAATCAATTACAGATTTCTCCGTGATAGGTATCGATGAAGGACAGTTCTTTCCAGACATTGTTGAATTC
TGTGAGCGTATGGCAAACGAAGGAAAAATAGTTATAGTAGCCGCACTCGATGGGACATTTCAACGTAAAC
CGTTTAATAATATTTTGAATCTTATTCCATTATCTGAAATGGTGGTAAAACTAACTGCTGTGTGTATGAA
ATGCTTTAAGGAGGCTTCCTTTTCTAAACGATTGGGTGAGGAAACCGAGATAGAAATAATAGGAGGTAAT
GATATGTATCAATCGGTGTGTAGAAAGTGTTACATCGACTCATA
Protein Sequence 
>gi|66275891|ref|YP_232976.1| thymidine kinase [Vaccinia virus]
MNGGHIQLIIGPMFSGKSTELIRRVRRYQIAQYKCVTIKYSNDNRYGTGLWTHDKNNFEALEATKLCDVL
ESITDFSVIGIDEGQFFPDIVEFCERMANEGKIVIVAALDGTFQRKPFNNILNLIPLSEMVVKLTAVCMK
CFKEASFSKRLGEETEIEIIGGNDMYQSVCRKCYIDS
Molecule Role Virulence factor
Molecule Role Annotation We have shown that insertion of the three vaccinia virus (VACV) promoter-driven foreign gene expression cassettes encoding Renilla luciferase-Aequorea GFP fusion protein, β-galactosidase, and β-glucuronidase into the F14.5L, J2R, and A56R loci of the VACV LIVP genome, respectively, results in a highly attenuated mutant strain GLV-1h68. This strain shows tumor-specific replication and is capable of eradicating tumors with little or no virulence in mice. (Chen et al., 2011)
References
Chen et al., 2011: Chen NG, Yu YA, Zhang Q, Szalay AA. Replication efficiency of oncolytic vaccinia virus in cell cultures prognosticates the virulence and antitumor efficacy in mice. Journal of translational medicine. 2011; 9; 164. [PubMed: 21951588].