Victors

F12L
Victor ID	5020
Gene Name	F12L
Sequence Strain (Species/Organism)	Vaccinia virus
NCBI Gene ID	3707508
NCBI Protein GI	66275848
Locus Tag	VACWR051
Genbank Accession	AY243312
Protein Accession	YP_232933
Taxonomy ID	10245
Gene Starting Position	38889
Gene Ending Position	40796
Gene Strand (Orientation)	-
Protein Name	EEV maturation protein
Protein pI	6
Protein Weight	70237.94
Protein Length	635
Protein Note	EEV maturation protein; similar to vaccinia virus strain Copenhagen F12L; facilitates the transport of intracellular viral particles to the cell membrane; in its absence particles are assembled but remain intracellular and do not traffic to cell membrane; The poxviridae are enveloped unsegmented dsDNA viruses; unlike many dsDNA viruses that replicate in the host nucleus poxviruses encode their own replication machinery and therefore replicate in the cytoplasm; viral genes are expressed in a bi-phasic manner with early genes encoding non-structural proteins involved in genome replication and late genes encoding the viral structural proteins;
DNA Sequence	>gi\|66275797:38889-40796 Vaccinia virus, complete genome TTTATAATTTTACCATCTGACTCATGGATTCATTAATATCTTTATAAGAGCTACTAACGTATAATTCTTT ATAACTGAACTGAGATATATACACCGGATCTATGGTTTCCATAATTGAGTAAATGAATGCTCGGCAATAA CTAATGGCAAATGTATAGAACAACGAAATTATACTAGAGTTGTTAAAGTTAATATTTTCTATGAGCTGTT CCAATAAATTATTTGTTGTAACTGCGTTCAAGTCATAAATCATCTTGATACTATCCAGTAAACCGTGTTT AAGTTCTGGAATATTATCATCCCATTGTAAAGCCCCTAATTCGACTATCGAATATCCTGCTCTGATAGCA GTTTCAATATCGACGGACGTCAATACTGTAATAAAGGTGGTAGTATTGTCATCATCGTGATAAACTACGG GAATATGGTCGTTAGTAGGTACGGTGACTTTACACAACGCGATATATAACTTTCCTTTTGTACCATTTTT AACGTAGTTGGGACGTCCTGCAGGGTATTGTTTTGAAGAAATGATATCGAGAACAGATTTGATACGATAT TTGTTGGATTCCTGATTATTCACTATAATATAATCTAGACAGATAGATGATTCGATAAATAGAGAAGGTA TATCGTTGGTAGGATAATACATCCCCATTCCAGTATTCTCGGATACTCTATTGATGACACTAGTTAAGAA CATGTCTTCTATTCTAGAAAACGAAAACATCCTACATGGACTCATTAAAACTTCTAACGCTCCTGATTGT GTCTCGAATGCCTCGTACAAGGATTTCAAGGATGCCATAGATTCTTTGACCAACGATTTAGAATTGCGTT TAGCATCTGATTTTTTTATTAAATCGAATGGTCGGCTCTCTGGTTTGCTACCCCAATGATAACAATAGTC TTGTAAAGATAAACCGCAAGAAAATTTATACGCATCCATCCAAATAACCCTAGCACCATCGGATGATATT AATGTATTATTATAGATTTTCCATCCACAATTATTGGGCCAGTATACTGTTAGCAACGGTATATCGAATA GATTACTCATGTAACCTACTAGAATGATAGTTCGTGTACTAGTCATAATATCTTTAATCCAATCTAAGAA ATTTAAAATTAGATTTTTTACACTGTTAAAGTTAACAAAGGTATTACCCGGATACGTGGATATCATATAT GGCATTGGTCCATTATCAGTAATAGCTCCATAAACTGATACGGCGATGGTTTTTATATGTGTTTGATCTA ACGAGGAAGAAATTCGCGCCCACAATTCATCTCTAGATATGTATTTAATATCAAACGGTAACACATCAAT TTCGGGACGCGTATATGTTTCTAAATTTTTAATCCAAATATAATGATGACCTATATGCCCTATTATCATA CTGTCAACTATAGTACACCTAGAGAACTTACGATACATCTGTTTCCTATAATCGTTAAATTTTACAAATC TATAACATGCTAAACCTTTTGACGACAGCCATTCATTAATTTCTGATATGGAATCTGTATTCTCGATACC GTATTGTTCTAAAGCCAGTGCTATATCTCCCTGTTCGTGGGAACGCTTTCGTATAATATCGATCAACGGA TAATCTGAAGTTTTTGGAGAATAATATGACTCATGATCTATTTCGTCCATAAACAATCTAGACATAGGAA TTGGAGGCGATGATCTTAATTTTGTGCAATGAGTCGTCAATCCTATAACTTCTAATCTTGTAATATTCAT CATCGACATAATACTATCTATGTTATCATCGTATATTAGTATACCACGGCCTTCTTCATTTCGTGCCAAA ATAATATACAGTCTTAAATAATTACGCAATATCTCAATAGTTTCATAATTGTTAGCTGTTTTCATCAAGA TTTGTACCCTGTTTAACA
Protein Sequence	>gi\|66275848\|ref\|YP_232933.1\| involved in plaque and EEV formation [Vaccinia virus] MLNRVQILMKTANNYETIEILRNYLRLYIILARNEEGRGILIYDDNIDSIMSMMNITRLEVIGLTTHCTK LRSSPPIPMSRLFMDEIDHESYYSPKTSDYPLIDIIRKRSHEQGDIALALEQYGIENTDSISEINEWLSS KGLACYRFVKFNDYRKQMYRKFSRCTIVDSMIIGHIGHHYIWIKNLETYTRPEIDVLPFDIKYISRDELW ARISSSLDQTHIKTIAVSVYGAITDNGPMPYMISTYPGNTFVNFNSVKNLILNFLDWIKDIMTSTRTIIL VGYMSNLFDIPLLTVYWPNNCGWKIYNNTLISSDGARVIWMDAYKFSCGLSLQDYCYHWGSKPESRPFDL IKKSDAKRNSKSLVKESMASLKSLYEAFETQSGALEVLMSPCRMFSFSRIEDMFLTSVINRVSENTGMGM YYPTNDIPSLFIESSICLDYIIVNNQESNKYRIKSVLDIISSKQYPAGRPNYVKNGTKGKLYIALCKVTV PTNDHIPVVYHDDDNTTTFITVLTSVDIETAIRAGYSIVELGALQWDDNIPELKHGLLDSIKMIYDLNAV TTNNLLEQLIENINFNNSSIISLFYTFAISYCRAFIYSIMETIDPVYISQFSYKELYVSSSYKDINESMS QMVKL
Molecule Role	Virulence factor
Molecule Role Annotation	The vDeltaF12L virus was severely attenuated in vivo, such that a dose of vDeltaF12L 10,000-fold greater than the dose of wild-type virus that induced severe disease was unable to induce disease in mice infected intranasally (Zhang et al., 2000).
References	*Zhang et al., 2000:* Zhang WH, Wilcock D, Smith GL. Vaccinia virus F12L protein is required for actin tail formation, normal plaque size, and virulence. Journal of virology. 2000; 74(24); 11654-11662. [PubMed: 11090164].


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F12L