The mutant strain was constructed from the parental strain L. monocytogenes EGD-e using a two step integration/excision procedure. In-frame deletion of agrD resulted in an altered adherence and biofilm formation in abiotic surfaces
Protein Sequence
>gi|157939819|emb|CAL81507.1| accessory gene regulator protein D [Listeria monocytogenes]
MKNMNKSVGKFLSRLASCLYTNQKVHL
Molecule Role
Virulence factor
Molecule Role Annotation
MUTATION: agrD mutant reduced invasion of Caco-2 intestinal epithelial cells, attenuated in mice (Riedel et al., 2009).
References
Riedel et al., 2009: Riedel CU, Monk IR, Casey PG, Waidmann MS, Gahan CG, Hill C. AgrD-dependent quorum sensing affects biofilm formation, invasion, virulence and global gene expression profiles in Listeria monocytogenes. Molecular microbiology. 2009; 71(5); 1177-1189. [PubMed: 19154329].
