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purN

Victor ID 4495
Gene Name purN
Sequence Strain (Species/Organism) Pasteurella multocida subsp. multocida str. Pm70
NCBI Gene ID 1243367
NCBI Protein GI 15601885
Locus Tag PM0020
Genbank Accession AE004439
Protein Accession NP_244957
Taxonomy ID 272843
Gene Starting Position 16765
Gene Ending Position 17406
Gene Strand (Orientation) -
Protein Name phosphoribosylglycinamide formyltransferase
Protein pI 6.16
Protein Weight 21918.48
Protein Length 213
Protein Note glycinamide ribonucleotide transformylase; GAR Tfase; catalyzes the synthesis of 5'-phosphoribosylformylglycinamide from 5'-phosphoribosylglycinamide and 10-formyltetrahydrofolate; PurN requires formyl folate for the reaction unlike PurT which uses formate
DNA Sequence 
>gi|15601865:16765-17406 Pasteurella multocida subsp. multocida str. Pm70, complete genome
TTTATTCTTCCATCGCATACCCTTGTGGGGGCAATTGTTTGCCATCAAGGAAAGCATGTTCACCGATCAA
CGTTAAACGCTCCTCTACAAACCATTTGACGACTAAAGGATAAATGCGTAACTCTTGCGCTTTGACACGT
TGCTCAATGTCTTCAATACGGTCTTCAGCAAAGATAGGCACTTTCGCTTGTAAAATGACGGCACCTGCAT
CCACCTCTTCATTCACAAAATGGACTGAGGTACCATGTTCGCGCTCACCTGCATCGAGGGCTTGCTGATA
AGTATTGAGACCCGGGTATTTAGGGAGAAGCGAAGGATGAATATTGAGAATTTTGCCAGCAAAACGTTGA
GTAAACGCAGGACTTAAAATTTTCATATAGCCTGCGAGTACGATCAAATCGGCTTGGACAGATTCAATAT
AATGACCAATTTGCTCATCCATAGCACGGTTATTAGCAAAATCTTTGCGTAAAAACACTTTACTTGGAAT
GGACGCGCTTTGAGCTCGCTCTAAGGCATAAGCCTCCGCTTGATTACTGATGACAGCAACGATTTTACCT
GCAATCTGTCCGCTATGACAGGCATCGATTAATGCCTGTAAATTACTGCCATGTCCTGACACTAAAACAA
CAATTTTTTTCA
Protein Sequence 
>gi|15601885|ref|NP_244957.1| phosphoribosylglycinamide formyltransferase [Pasteurella multocida subsp. multocida str. Pm70]
MKKIVVLVSGHGSNLQALIDACHSGQIAGKIVAVISNQAEAYALERAQSASIPSKVFLRKDFANNRAMDE
QIGHYIESVQADLIVLAGYMKILSPAFTQRFAGKILNIHPSLLPKYPGLNTYQQALDAGEREHGTSVHFV
NEEVDAGAVILQAKVPIFAEDRIEDIEQRVKAQELRIYPLVVKWFVEERLTLIGEHAFLDGKQLPPQGYA
MEE
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: purN mutant is attenuated in chickens and mice (Harper et al., 2003).
References
Harper et al., 2003: Harper M, Boyce JD, Wilkie IW, Adler B. Signature-tagged mutagenesis of Pasteurella multocida identifies mutants displaying differential virulence characteristics in mice and chickens. Infection and immunity. 2003; 71(9); 5440-5446. [PubMed: 12933901].