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hemH from Brucella melitensis biovar Abortus 2308

Victor ID 1772
Gene Name hemH from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3828247
NCBI Protein GI 83269029
Locus Tag BAB2_0075
Protein Accession YP_418320.1
Other Database IDs UniProtKB-ID: HEMH_BRUA2
UniRef100: UniRef100_B2SCP3
UniRef90: UniRef90_B2SCP3
UniRef50: UniRef50_A9MDJ3
UniParc: UPI00004F9274
EMBL: AM040265
EMBL-CDS: CAJ12241.1
RefSeq_NT: NC_007624.1
GenomeReviews: AM040265_GR
KEGG: bmf:BAB2_0075
eggNOG: COG0276
HOGENOM: HBG697135
OMA: TIEEIGM
ProtClustDB: PRK00035
BioCyc: BMEL359391:BAB2_0075-MONOMER
Taxonomy ID 359391
Chromosome No II
Gene Starting Position 74577
Gene Ending Position 75635
Gene Strand (Orientation) -
Protein Name ferrochelatase
DNA Sequence 
>gi|83268957:74577-75635 Brucella melitensis biovar Abortus 2308 chromosome II, complete genome
TCTAAACCCATCCCAGCAATTCGCGGCGCACCAGCGTCTCAATGACCTTCATGCCCTCTTCGCTATCGTT
GAGGCAGGGAATATGGCTGAAGTTTTCGCCGCCGTTTTCAAGAAACTCTTCCGCTGCCTCATTGCCGATT
TCGTCCACCGTTTCCAGGCAATCGGCCACGAAACCAGGATTGAGCACCGCCACCAATTTGACGCCGTTTT
TGGCCAGTTCCTTCACGGTTTCGTCGGTATAGGGCTGGAGCCATTCTTCCGGGCCGAAGCGTGACTGGAA
GGTGGCGCGCATCTCGTCTTCGCCAAGGCCGAGGCGCTCACGCAGAAGCCGCGTCGTTTCAAGGCATTGC
TGGCGATAGGGGTCGCCCTTGTCCGAATAGCTTTTCGGAATGCCGTGATAGGAGGTGAGGATGACTTCGG
GCTTGAAGGAAAGCGTAGCCAGATGCTTTTCGACGGAACGCGCCAGCGCGTCGATATAGACCGGCTCGGC
CTCGTATGACGGCACGGTGCGGATTGCAGGCATGAAGCGCTTTTTCATCAGCGCCTCGAAGAACTTGTCG
TTCACCGTCGCCGTGGTGGTGGCGGAATATTGCGGATAGAGCGGGAATATCACAATCCGCTCGCAGCCCT
GCTGAAGCAGGCGGTCGGTTATGCTCTCGATGGAAGGCTGGCCATAGCGCATCGCCCAGTCCACCACGAC
ATTGGGTTGGTCGCTAAGTGCTTTGGCAAGTTTCTCGCCCTGCGCGCGCGTATAGGTGCGCAGGGGCGAT
TCATTATTCTCGTGATTCCAGATGCGATCATAGAGCCTGCCGGAACGCCTGGGCCGTGTGTTGAGAACAA
TACCGTAAAGGATCGGGTACCAGATAAGGCGCGACCATTCGATGACGCGGCGGTCGGAGAGAAATTCGGC
AAGATAGCGCCGCATCGGGCCGTAGCTCGTGCCGTCCGGCGTGCCGAGATTGACCAGCAAAACCCCGACT
TTCGGCAGTTTGGCCGATGTATGCAGCGGGGTCTGCGCTGTCTGGCTGACGTTCACACGCACCTTGTCCG
TTCCACTCA
Protein Sequence 
>gi|83269029|ref|YP_418320.1| ferrochelatase [Brucella abortus 2308] MSGTDKVRVNVSQTAQTPLHTSAKLPKVGVLLVNLGTPDGTSYGPMRRYLAEFLSDRRVIEWSRLIWYPILYGIVLNTRPRRSGRLYDRIWNHENNESPLRTYTRAQGEKLAKALSDQPNVVVDWAMRYGQPSIESITDRLLQQGCERIVIFPLYPQYSATTTATVNDKFFEALMKKRFMPAIRTVPSYEAEPVYIDALARSVEKHLATLSFKPEVILTSYHGIPKSYSDKGDPYRQQCLETTRLLRERLGLGEDEMRATFQSRFGPEEWLQPYTDETVKELAKNGVKLVAVLNPGFVADCLETVDEIGNEAAEEFLENGGENFSHIPCLNDSEEGMKVIETLVRRELLGWV
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(UniProt: P0A3D7).

CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(UniProt: P0A3D7).

PATHWAY: Protoheme biosynthesis; last step(UniProt: P0A3D7).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P0A3D7).

SIMILARITY: Belongs to the ferrochelatase family(UniProt: P0A3D7).

MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme (Almirón et al., 2001).
COG COG0276H, under H: Coenzyme transport and metabolism
References
Almirón et al., 2001: Almirón M, Martínez M, Sanjuan N, Ugalde RA. Ferrochelatase is present in Brucella abortus and is critical for its intracellular survival and virulence. Infection and immunity. 2001; 69(10); 6225-6230. [PubMed: 11553564].