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sodC from Brucella melitensis biovar Abortus 2308

Victor ID 1754
Gene Name sodC from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3827840
NCBI Protein GI 83269434
Locus Tag BAB2_0535
Protein Accession YP_418725.1
Other Database IDs UniProtKB-ID: Q2YKV9_BRUA2
UniRef100: UniRef100_P66826
UniRef90: UniRef90_P66826
UniRef50: UniRef50_P0AGD2
UniParc: UPI000016780C
EMBL: AM040265
EMBL-CDS: CAJ12701.1
RefSeq_NT: NC_007624.1
GenomeReviews: AM040265_GR
KEGG: bmf:BAB2_0535
eggNOG: COG2032
HOGENOM: HBG609879
OMA: GEHAASM
ProtClustDB: CLSK898879
BioCyc: BMEL359391:BAB2_0535-MONOMER
Taxonomy ID 359391
Chromosome No II
Gene Starting Position 534068
Gene Ending Position 534589
Gene Strand (Orientation) +
Protein Name Copper/Zinc superoxide dismutase
DNA Sequence 
>gi|83268957:534068-534589 Brucella melitensis biovar Abortus 2308 chromosome II, complete genome
GATGAAGTCCTTATTTATTGCATCGACAATGGTGCTTATGGCTTTTCCGGCTTTCGCAGAAAGCACGACG
GTAAAAATGTATGAGGCGCTGCCGACCGGACCGGGTAAAGAAGTTGGCACCGTGGTCATTTCCGAAGCCC
CGGGCGGGCTGCACTTCAAGGTGAATATGGAAAAGCTGACGCCGGGCTATCATGGCTTTCATGTTCACGA
AAATCCAAGCTGCGCTCCGGGAGAAAAAGACGGCAAGATCGTACCGGCTCTTGCTGCCGGCGGGCATTAT
GATCCGGGTAATACCCATCACCATTTAGGACCTGAAGGTGATGGACATATGGGCGATTTGCCACGCCTGA
GCGCCAATGCTGACGGCAAGGTGAGTGAAACCGTTGTCGCTCCACATCTCAAGAAATTGGCGGAAATCAA
GCAGCGTTCTTTGATGGTCCATGTCGGAGGGGATAATTATTCCGATAAGCCTGAGCCGCTTGGTGGCGGT
GGTGCCCGTTTTGCCTGCGGCGTGATCGAATA
Protein Sequence 
>gi|83269434|ref|YP_418725.1| copper/Zinc superoxide dismutase [Brucella abortus 2308] MKSLFIASTMVLMAFPAFAESTTVKMYEALPTGPGKEVGTVVISEAPGGLHFKVNMEKLTPGYHGFHVHENPSCAPGEKDGKIVPALAAGGHYDPGNTHHHLGPEGDGHMGDLPRLSANADGKVSETVVAPHLKKLAEIKQRSLMVHVGGDNYSDKPEPLGGGGARFACGVIE
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)(UniProt: P66827).

CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2)(UniProt: P66827).

COFACTOR: Binds 1 copper ion per subunit (By similarity)(UniProt: P66827).

COFACTOR: Binds 1 zinc ion per subunit (By similarity)(UniProt: P66827).

SUBUNIT: Homodimer (By similarity)(UniProt: P66827).

SUBCELLULAR LOCATION: Periplasmic (By similarity)(UniProt: P66827).

SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family(UniProt: P66827).

IMMUNOGENICITY: Induces antigen-specific Th1 immune response, as indicated by the specific induction of serum IgG2a, but not IgG1, antibodies and by the secretion of IFN-γ, but not IL-4, by the Cu/Zn SOD-stimulated splenocytes. Has been used for vaccine development (Vemulapalli et al., 2000; Vemulapalli et al., 2000; Vemulapalli et al., 2000).

MUTATION: An isogenic sodC mutant constructed from B abortus 2308 by gene replacement exhibited much greater susceptibility to killing by exogenous O(2)(-) than the parental 2308 strain, supporting a role for SodC in protecting this bacterium from O(2)(-) stress. The B abortus sodC mutant was much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and its attenuation in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon. The attenuation of the B abortus sodC mutant in both resting and IFN-gamma -activated macrophages was alleviated in the presence of the NADPH oxidase inhibitor apocynin. Consistently, the B abortus sodC mutant also displayed significant attenuation in infected C57BL6J mice compared to the parental strain. These findings suggest that SodC protects B abortus 2308 from the respiratory burst of host macrophages (Vemulapalli et al., 2000).
COG COG2032P, under P: Inorganic ion transport and metabolism
References
Vemulapalli et al., 2000: Vemulapalli R, He Y, Cravero S, Sriranganathan N, Boyle SM, Schurig GG. Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51. Infection and immunity. 2000; 68(6); 3286-3289. [PubMed: 10816475].