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znuA from Brucella melitensis biovar Abortus 2308

Victor ID 1750
Gene Name znuA from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3827700
NCBI Protein GI 83269918
Locus Tag BAB2_1079
Protein Accession YP_419209.1
Other Database IDs UniProtKB-ID: ZNUA_BRUA2
UniRef100: UniRef100_Q2YJH5
UniRef90: UniRef90_Q2YJH5
UniRef50: UniRef50_Q2YJH5
UniParc: UPI00004F944F
EMBL: AY941821
EMBL: AM040265
EMBL-CDS: AAX46323.1
EMBL-CDS: CAJ13245.1
RefSeq_NT: NC_007624.1
GenomeReviews: AM040265_GR
KEGG: bmf:BAB2_1079
eggNOG: COG4531
HOGENOM: HBG731008
OMA: AYQYFEH
ProtClustDB: CLSK886373
BioCyc: BMEL359391:BAB2_1079-MONOMER
Taxonomy ID 359391
Chromosome No II
Gene Starting Position 1063213
Gene Ending Position 1064217
Gene Strand (Orientation) -
Protein Name Periplasmic solute binding protein
DNA Sequence 
>gi|83268957:1063213-1064217 Brucella melitensis biovar Abortus 2308 chromosome II, complete genome
TTTATTTCGGCAGGCAGTCTTTCAGCGAATTGGCCAGATTGCGGATGAGTTGCGGATAAAGATCAGGGCC
GTCCTTCAGTTCTGCGCCAAGAGGATCAAGAACACCTGTCCGGGCTTTCGTTCCGTCAACCACCGTCTTC
ACCAGTTTCGGCTCAAATTGCGGCTCTGAGAAAACGCAGGTTGCACCGAGTGACTTTATCTTGTCGTGAA
TTTGCTGGATACGCGCCGCACCCGGCGCTTTTTCCGGGCTGACCGTGATGGAGCCGGCTGCTTTCATGCC
GAAGCGATTCTCGAAATATTGATAGGCATCATGAAAGACGATGAAGGGCTTGTCCTTGACCGGCTTCAGT
TCAGCAGCCACTTCCCTGGTCAAGGCATCGAGTTTTTCTCCATAGGCCTTGGCATTCTTTTCATATTGCG
CGGCGTGTTCCGGGTCGCTCTCGCCAAGCGTTTTGGCGATATCGGCAGCCAGAATCTTGCCATTTTGCGG
GTCAAGCCAGAAATGCAGATCATATTCGCCGTGATGGTGGTGTTCATGGCCTTCTTCTGCGACTTCGGCC
GCATGGTCGTGATCGCCTGAACCATGTGCGTCATGCTCTTCCTCATGGCTCCCCCCATGGCCATGATCGT
GCGCCTCGAACGGCCCACCCTCGCGGAATTTTAGTTTCGTCAACCCTTTCGCATCGCCAAGAGCCACCAC
TTTCGCGCCCTCGCCCAGCGTATCAATGGGCTTGTCGAGAAAGGTTTCCATGGATGGCCCGGCCCAGAAA
ATCACCTTGGCGTGCTCGATAGCCTCGGCATCGGAGGGTTTCAGACTATAAACATGCTCGGACCCAGCCC
CCTGAACGATCAGCTTCGGCTTACCCACGCCCTGCATGACGGCGGAAACAATCGAGTGAAGCGGCTTGAT
CGAAACAACCACGCCCTCACGTTCGCCTGCAAGGGAAGAACCGCAAAAGCCAGCCAGAAAGGCAGAAGCA
AGAAACAAGGAATGCAGATTTTTCA
Protein Sequence 
>gi|83269918|ref|YP_419209.1| periplasmic solute binding protein [Brucella abortus 2308] MKNLHSLFLASAFLAGFCGSSLAGEREGVVVSIKPLHSIVSAVMQGVGKPKLIVQGAGSEHVYSLKPSDAEAIEHAKVIFWAGPSMETFLDKPIDTLGEGAKVVALGDAKGLTKLKFREGGPFEAHDHGHGGSHEEEHDAHGSGDHDHAAEVAEEGHEHHHHGEYDLHFWLDPQNGKILAADIAKTLGESDPEHAAQYEKNAKAYGEKLDALTREVAAELKPVKDKPFIVFHDAYQYFENRFGMKAAGSITVSPEKAPGAARIQQIHDKIKSLGATCVFSEPQFEPKLVKTVVDGTKARTGVLDPLGAELKDGPDLYPQLIRNLANSLKDCLPK
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Brucella abortus znuA mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells (Kim et al., 2003). High-affinity zinc uptake system protein mutant (znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. These results suggest that znuA does not contribute to intracellular trafficking of B abortus, but contributes to utilization of zinc required for intracellular growth (Kim et al., 2003).
COG COG4531P, under P: Inorganic ion transport and metabolism
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].