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rpoA from Brucella melitensis biovar Abortus 2308

Victor ID 1744
Gene Name rpoA from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788935
NCBI Protein GI 82700043
Locus Tag BAB1_1231
Protein Accession YP_414617.1
Other Database IDs UniProtKB-ID: RPOA_BRUA2
UniRef100: UniRef100_B2S655
UniRef90: UniRef90_B2S655
UniRef50: UniRef50_Q5HAU5
UniParc: UPI00004F908C
EMBL: AM040264
EMBL-CDS: CAJ11187.1
RefSeq_NT: NC_007618.1
HSSP: P20429
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1231
eggNOG: COG0202
HOGENOM: HBG430844
OMA: FGTTLGN
ProtClustDB: PRK05182
BioCyc: BMEL359391:BAB1_1231-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1202049
Gene Ending Position 1203062
Gene Strand (Orientation) -
Protein Name DNA-directed RNA polymerase subunit alpha
DNA Sequence 
>gi|82698932:1202049-1203062 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
CTCAATATTGGTCTTCGTAGCGCTTGGCGAGGTCTTCGATATTTTCCGGCGGCCAGGCTGGGATTTCCAT
ACCGAGATGGAGACCCATCGATGCCAGAACTTCCTTGATCTCGTTAAGCGACTTGCGGCCGAAATTCGGG
GTCCGCAGCATTTCGGCTTCCGTCTTCTGGATCAGATCGCCGATATAGACGATGTTGTCGTTCTTCAGGC
AGTTTGCCGAACGCACCGACAGCTCCAGTTCGTCCACCTTCTTGAGCAGAGCCGGATTGAAGGCCAGTTC
CGCAACCTGTTCCTGCGGAGCTTCCTTCTGCGGCTCTTCAAAGTTGACGAAGATCGAAAGCTGGTCCTGG
AGAATGCGAGCGGCATAAGCCACAGCGTCTTCACCCGTAACCGAACCATTGGTCTCGATATTCAGCGTCA
GCTTGTCATAGTCGAGAACCTGGCCTTCACGGGTGTTCTCGATCTTGTACGACACCTTGCGAACCGGAGA
ATAAAGGCTATCGACCGGGATCAGACCGATCGGAGCATCTTCCGCGCGGTTGCGGTCGGCAGGCACATAT
CCCTTGCCGGTGTTGACGGTGAATTCCATGCGGATTTCAGCGCCTTCATCCAGCGTGCAGATGACGTGTT
CCGGGTTCAGGATCTCGACATCGCCAACGGTCTGAATGTCGCCAGCCGTAACGACGCCGGGGCCTTCCTT
GCGGACGACCATGCGCTTCGGGCCCTCGCCTTCCATACGGATGGCGATTTCCTTGATGTTCAGAACGATA
TCCGTGACGTCTTCGCGAACGCCCGGAATGGAAGAGAATTCATGCAGAACGCCGTCGATCTGGACAGCGG
TCACGGCAGCGCCGCGAAGCGACGACAGAAGCACGCGACGCAGCGCATTGCCGAGCGTCAGGCCAAAGCC
GCGTTCCAGCGGCTCAGCGACGACGGTTGCATGCGTGCGGGAGCCGTGGGTGATGAAATCCACCTTGTTC
GGCTTGATCAGTTCCTGCCAGTTCTTCTGGATCA
Protein Sequence 
>gi|82700043|ref|YP_414617.1| DNA-directed RNA polymerase subunit alpha [Brucella abortus 2308] MIQKNWQELIKPNKVDFITHGSRTHATVVAEPLERGFGLTLGNALRRVLLSSLRGAAVTAVQIDGVLHEFSSIPGVREDVTDIVLNIKEIAIRMEGEGPKRMVVRKEGPGVVTAGDIQTVGDVEILNPEHVICTLDEGAEIRMEFTVNTGKGYVPADRNRAEDAPIGLIPVDSLYSPVRKVSYKIENTREGQVLDYDKLTLNIETNGSVTGEDAVAYAARILQDQLSIFVNFEEPQKEAPQEQVAELAFNPALLKKVDELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEIPAWPPENIEDLAKRYEDQY
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates(UniProt: Q8G094).

CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)(UniProt: Q8G094).

SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity)(UniProt: Q8G094).

DOMAIN: The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements (By similarity)(UniProt: Q8G094).

SIMILARITY: Belongs to the RNA polymerase alpha chain family(UniProt: Q8G094).

MUTATION: The rpoA gene codes for the essential alpha-subunit of the RNA polymerase. B. melitensis rpoA mutant was found by signature-tagged mutagenesis from a mouse infection model. This disruption leaves a partially functional protein, impaired for the activation of virB transcription, as demonstrated by the absence of induction of the virB promoter in the Tn5::rpoA background. RpoA is involved in virB regulation in vitro. The mutant (Tn5::rpoA) was more resistant to oxidative stress (Lestrate et al., 2003).
COG COG0202K, under K: Transcription
References
Lestrate et al., 2003: Lestrate P, Dricot A, Delrue RM, Lambert C, Martinelli V, De Bolle X, Letesson JJ, Tibor A. Attenuated signature-tagged mutagenesis mutants of Brucella melitensis identified during the acute phase of infection in mice. Infection and immunity. 2003; 71(12); 7053-7060. [PubMed: 14638795].