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recA from Brucella melitensis biovar Abortus 2308

Victor ID 1741
Gene Name recA from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788897
NCBI Protein GI 82700036
Locus Tag BAB1_1224
Protein Accession YP_414610.1
Other Database IDs UniProtKB-ID: RECA_BRUA2
UniRef100: UniRef100_B2S648
UniRef90: UniRef90_B2S648
UniRef50: UniRef50_B2S648
UniParc: UPI00001334D4
EMBL: AM040264
EMBL-CDS: CAJ11180.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1224
eggNOG: COG0468
HOGENOM: HBG339889
OMA: GRDNTIT
ProtClustDB: PRK09354
BioCyc: BMEL359391:BAB1_1224-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1195422
Gene Ending Position 1196507
Gene Strand (Orientation) -
Protein Name recombinase A
DNA Sequence 
>gi|82698932:1195422-1196507 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
CTTACATTTCCGCCGCGCCGGCAGCATCTTCTTCCGGTCCGCCATCGTCAAGGAACTGCTCGGCGATCAG
GCCGGCATTCTGGCGAAGCGTGGTTTCGATTTCACGCGCGACTTCCGGATTATCCTTGAGATATTGCTTG
GCGTTCTCGCGCCCCTGCCCGAGACGCTGGGAATTATAGGAGAACCAGGCACCAGACTTCTCAACCACAC
CGGCCTTGACACCAAGATCGACCAGCTCGCCCACCTTGGAAACGCCCGCGCCATACATGATATCGAATTC
GACCTGCTTGAACGGAGGAGCGAGCTTGTTCTTCACCACCTTCACGCGGGTCTGGTTGCCCACCACTTCG
TCGCGCTCCTTGATGGAGCCGATACGGCGAATATCGAGGCGAACCGAAGCATAGAACTTGAGCGCATTGC
CGCCCGTTGTCGTTTCCGGCGAGCCGAACATGACACCGATCTTCATGCGGATCTGGTTGATGAAGATCAC
CATGCAGTTTGAACGGGAAATGGAGCCAGTAAGCTTGCGCAGCGCCTGGCTCATGAGACGGGCCTGAAGG
CCCGGCAGGGAATCCCCCATTTCACCTTCGATTTCAGCACGCGGCGTCAGGGCTGCGACCGAGTCGACAA
CGAGAACGTCGATGGCGCCAGAGCGCACGAGCGTATCGGTGATTTCAAGCGCCTGTTCACCCGTATCGGG
CTGCGAGATCAGAAGATTTTCAAGATCGACACCGAGCTTGCGCGCATAGACCGGGTCAAGCGCATGTTCC
GCATCGACGAATGCGCAGATGCCGCCCTTCTTCTGCGCTTCAGCGATGGTGTGCAAGGCAAGCGTGGTTT
TACCGGAACTTTCCGGCCCATAGATTTCCACGATACGTCCCTTGGGCAAGCCGCCAACCCCCAAGGCAAT
ATCGAGCGAGAGCGAGCCGGTCGATACGGTTTCGATTTCGACCACCTGATCGTTCTGGCCCAGACGCATG
ATTGAGCCTTTGCCGAACGCCCGTTCGATTTGCGACAATGCCGCGTCGAGAGCTTTTGTCTTGTCCACTG
AATTATCCTCAACAAGTCGCAATGAATTTTGAGACA
Protein Sequence 
>gi|82700036|ref|YP_414610.1| recombinase A [Brucella abortus 2308] MSQNSLRLVEDNSVDKTKALDAALSQIERAFGKGSIMRLGQNDQVVEIETVSTGSLSLDIALGVGGLPKGRIVEIYGPESSGKTTLALHTIAEAQKKGGICAFVDAEHALDPVYARKLGVDLENLLISQPDTGEQALEITDTLVRSGAIDVLVVDSVAALTPRAEIEGEMGDSLPGLQARLMSQALRKLTGSISRSNCMVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGAGVSKVGELVDLGVKAGVVEKSGAWFSYNSQRLGQGRENAKQYLKDNPEVAREIETTLRQNAGLIAEQFLDDGGPEEDAAGAAEM
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage (By similarity)(UniProt: P65976).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P65976).

SIMILARITY: Belongs to the recA family(UniProt: P65976).

MUTATION: The RecA mutant was more sensitive than the parental strain to killing by MMS. When administered intraperitoneally to BALBc mice, numbers of bacteria per spleen were consistently lower in animals infected with the RecA mutant than with the parental strain. However, both the RecA mutant and parental strain persisted in mice through 100 days post-infection. These results indicate that RecA is not crucial for persistence of B abortus in mice (Tatum et al., 1993).

The B abortus RecA mutant was virulent in mice, but its course of infection in mice differed from that of the parental strain. The infectious cycle of the parental strain in the mouse model was biphasic. During the rst week, there was an initial rise in cfu of B abortus 2308 in the spleen followed by a decrease during the second week. This phase was followed by a second phase in which B abortus S2308 persisted and slowly increased in numbers in the spleen . Though fewer RecA mutants were found in the spleens of mice infected intraperitoneally in the early stages of the infection and no large initial rise was seen, the same numbers were found as the parental strain 100 days post -infection. This suggests that collectively, different loci are involved to varying extents in the initial infection and the persistence phase (Tatum et al., 1993).

COG COG0468L, under L: Replication, recombination and repair
References
Tatum et al., 1993: Tatum FM, Morfitt DC, Halling SM. Construction of a Brucella abortus RecA mutant and its survival in mice. Microbial pathogenesis. 1993; 14(3); 177-185. [PubMed: 8321120].