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purD from Brucella melitensis biovar Abortus 2308

Victor ID 1734
Gene Name purD from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788874
NCBI Protein GI 82699328
Locus Tag BAB1_0442
Protein Accession YP_413902.1
Other Database IDs UniProtKB-ID: Q2YMD3_BRUA2
UniRef100: UniRef100_B2S9Q5
UniRef90: UniRef90_Q8YFK1
UniRef50: UniRef50_P52420
UniParc: UPI00004F8F0E
EMBL: AM040264
EMBL-CDS: CAJ10398.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_0442
eggNOG: COG0151
HOGENOM: HBG404060
OMA: FSLMAFV
ProtClustDB: PRK00885
BioCyc: BMEL359391:BAB1_0442-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 437132
Gene Ending Position 438415
Gene Strand (Orientation) -
Protein Name phosphoribosylamine--glycine ligase
DNA Sequence 
>gi|82698932:437132-438415 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
ATCAGCGATTAGCCTTCTCACGCTCCACGGCGCGCCAGCCGATATCAGAACGGTAAAAACCTTCCGGCCA
GTCGATGAGCTTCAGCGCCTCATAGGCACGCGCCTGCGCTTGCGCTACGGTTGCCGCCGTAGCGGTGATG
TTGAGGACGCGCCCGCCGCTGGCGACGATGGCGCCGTCCTTCAGCGCCGTGCCCGCATGGAACAGTTTTA
CGCCATCAATGCTTTCCAGCTTTTCGAGGTCGCGGATGACGCTGCCCTTCTTCACATTGGCCGGGTAGCC
TTCGGCGGCCATCACTACGGTCAGCGCCGGTTCGTCTTTCCATTCAAGTGAAACCTCATCAAGTCTGCCG
TCAACGGCAGCATTGATGAGGGCAAGCAGATCGCTGTTGAGGCGCATCATCAATACCTGGCATTCCGGAT
CGCCGAAACGGGTATTATATTCGATGAGCTTCGGGCCATCGCTGGTAATCATCAGCCCGGCAAAGAGAAT
GCCGGAAAAGGGTGCGCCGATTTCCGCCATGCCGCGCATGGTGGGTTCAATCAGTTCGCGCATGGTGCGC
GCCACCATTTCCGGTGTCATCACCGGGGCGGGCGCATAGGCGCCCATGCCGCCGGTATTCGGGCCGGTAT
CGCCATCGCCGACGCGCTTGTGATCCTGCGCGGAGCCAAGCGGCAATGCCGTCTTGCCATCGCAGATGCA
GAAGAAGCTTGCTTCCTCACCGTCAAGAAATTCCTCGACCACCACTTCCGCGCCTGCCGCACCGAAAGCG
CCCTCAAAGCAGGAATCGACCGCATCAAGTGCTTCCTGAAGCGTCATGGCGACAACCACGCCCTTGCCTG
CGGCAAGCCCATCGGCCTTGACCACGATGGGCGCGCCCTGCTGGCGGATATAGGCTTTCGCCTTTGGCGC
ATTGTTGAAACGGCCATAAGCGCCGGTCGGGATATTGAAGCGCGCGCAGAGATCCTTGGTGAAGCCCTTC
GAGCCTTCAAGCTGGGCTGCGGCCCTGGACGGGCCGAAGACGCGGATGCCCTCAGCGCGCATTTCATCGG
CAAGCCCGGCGACAAGCGGCGCTTCCGGCCCCACGACCACGAGGTCGATATGCTTTTCCTTGGCAAAGGC
AATCAGCGCGGCATGGTCGTCCACGCCGATATCGGCAAGCTCCGCTACAGCGGCGATACCGGGATTGCCG
GGCGCGCAATATAGTTTTTCAAGCAGGGGCGAGGCGGCAAGCTTCCATGCCAAGGCGTGCTCGCGACCAC
CGGAACCGATCAACAGAACTTTCA
Protein Sequence 
>gi|82699328|ref|YP_413902.1| phosphoribosylamine--glycine ligase [Brucella abortus 2308] MKVLLIGSGGREHALAWKLAASPLLEKLYCAPGNPGIAAVAELADIGVDDHAALIAFAKEKHIDLVVVGPEAPLVAGLADEMRAEGIRVFGPSRAAAQLEGSKGFTKDLCARFNIPTGAYGRFNNAPKAKAYIRQQGAPIVVKADGLAAGKGVVVAMTLQEALDAVDSCFEGAFGAAGAEVVVEEFLDGEEASFFCICDGKTALPLGSAQDHKRVGDGDTGPNTGGMGAYAPAPVMTPEMVARTMRELIEPTMRGMAEIGAPFSGILFAGLMITSDGPKLIEYNTRFGDPECQVLMMRLNSDLLALINAAVDGRLDEVSLEWKDEPALTVVMAAEGYPANVKKGSVIRDLEKLESIDGVKLFHAGTALKDGAIVASGGRVLNITATAATVAQAQARAYEALKLIDWPEGFYRSDIGWRAVEREKANR
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide(UniProt: Q8G2B1).

PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2(UniProt: Q8G2B1).

PATHWAY: Context: Purine biosynthesis(UniProt: Q8G2B1).

SIMILARITY: Belongs to the GARS family(UniProt: Q8G2B1).

SIMILARITY: Contains 1 ATP-grasp domain(UniProt: Q8G2B1).

MUTATION: Brucella abortus 2308 derivatives with mini-Tn5 insertions in purE, purL, and purD display significant attenuation in the BALBc mouse model. It confirms the importance of the purine biosynthesis pathways for the survival and replication of the brucellae in host macrophages (Drazek et al., 1995).

Like the purE mutant, a purD::Tn10 mutant has reduced survival in murine macrophages and reduced virulence in mice (Drazek et al., 1995).
COG COG0151F, under F: Nucleotide transport and metabolism
References
Drazek et al., 1995: Drazek ES, Houng HS, Crawford RM, Hadfield TL, Hoover DL, Warren RL. Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages. Infection and immunity. 1995; 63(9); 3297-3301. [PubMed: 7642258].