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leuC from Brucella melitensis biovar Abortus 2308

Victor ID 1725
Gene Name leuC from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788772
NCBI Protein GI 82700673
Locus Tag BAB1_1905
Protein Accession YP_415247.1
Other Database IDs UniProtKB-ID: LEUC_BRUA2
UniRef100: UniRef100_B2S860
UniRef90: UniRef90_B2S860
UniRef50: UniRef50_C1F700
UniParc: UPI00000DD71D
EMBL: AM040264
EMBL-CDS: CAJ11861.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1905
eggNOG: COG0065
HOGENOM: HBG330745
OMA: RPHAPKG
ProtClustDB: PRK05478
BioCyc: BMEL359391:BAB1_1905-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1854619
Gene Ending Position 1856028
Gene Strand (Orientation) -
Protein Name isopropylmalate isomerase large subunit
DNA Sequence 
>gi|82698932:1854619-1856028 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
CTCACTTCCACGCGCGAATATCGACGAAGTGACCGGCAATTGCAGCCGCCGCAGCCATTGCTGGCGATAC
CAGATGCGTACGACCCTTGAAGCCCTGACGGCCCTCGAAATTGCGGTTCGAGGTCGAAGCGCAACGCTCG
CCCGGCTTCAGCCGGTCATCGTTCATCGCAAGGCACATGGAGCAGCCCGGCTCGCGCCAGTCGAAACCGG
CTTCAATGAAAATCTTGTGCAGGCCTTCGGCTTCCGCCTGTTCCTTTACGAGACCCGAACCCGGAACGAT
CATGGCATTGACGGTCGGGGCAACCTTCTTGCCTTCCACGACCTTGGCGGCAGCGCGAAGGTCTTCGATG
CGGCCATTGGTGCACGAACCGATGAAGACGCGATCAACCGCGATATCCGTGATTTTCGTGCCGGGCTTCA
GGCCCATATAGTCGAGCGCACGCCACTTGGAGGCACGCTTGGTTTCGTCGGCGATATCGTCCGGATTGGG
CACGACCCCCGTGACGGAAACGACGTCTTCCGGCGAAGAACCCCAGGAAACGATGGGCGGCAGGCTGCCG
GCGTCGAGCGTCACGATCTTGTCGAAATGCGCCCCTTCATCCGAATGCAGCGTCTTCCAGTAATTGATCG
CCTGTTCCAGCGTTTCGCCCTGCGGAGCACGCGGGCGGCCCTTGATATATTCAAACGTCGTCTCGTCGGG
AGCGATAAGACCGGCACGCGCGCCGCCCTCGATCGACATGTTGCAGATCGTCATGCGGCCTTCCATCGAC
AGCGAGCGAATGGCTTCGCCCGCATATTCGATGACATAGCCGGTGCCGCCTGCCGTGCCGATCTCGCCAA
TGATCGCCAGAACAATATCCTTGGCGGTCACGCCCGCCGGCAACTTGCCGTCAACGCGCACCAGCATATT
CTTGGCTTTTTTCTGGATCAGCGTCTGGGTGGCGAGCACATGCTCCACCTCCGATGTGCCGATGCCATGT
GCAAGCGCACCGAAAGCGCCGTGGGTGGAAGTGTGGCTGTCACCGCAAACGATGGTCATGCCCGGCAGGG
TGAAGCCCTGCTCTGGCCCGACGATATGCACGATGCCCTGGCGCTTGTCGCGTTCGGAATAATATTCGAC
GCCGAAATCGGCGGCATTCCTGGCCAGTGCCTCGACCTGGATGCGGCTTTCCTCGTTCTGGATGCCATTG
ATGCGGTCGGGCGACGTCGGAACGTTATGGTCCACCACGGCAAGCGTCTTTTCCGGGTGGCGCACCGGGC
GGCCTGCCATGCGCAGCCCCTCAAAGGCCTGCGGGCTCGTCACTTCATGCACAAGATGGCGATCAATATA
AAGGAGGCAGGTGCCGTCTTCCTGCTGATCCACTACATGGTCATCCCAAATCTTGTCATAAAGTGTACGC
GGCGCGCTCA
Protein Sequence 
>gi|82700673|ref|YP_415247.1| isopropylmalate isomerase large subunit [Brucella abortus 2308] MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDETTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H(2)O(UniProt: Q8FYG9).

CATALYTIC ACTIVITY: (2S)-2-isopropylmaleate + H(2)O = 3-hydroxy-4-methyl-3-carboxypentanoate(UniProt: Q8FYG9).

COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity)(UniProt: Q8FYG9).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2(UniProt: Q8FYG9).

SUBUNIT: Heterodimer of leuC and leuD (By similarity)(UniProt: Q8FYG9).

SIMILARITY: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily(UniProt: Q8FYG9).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis leuC gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0065E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].