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leuA from Brucella melitensis biovar Abortus 2308

Victor ID 1724
Gene Name leuA from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788771
NCBI Protein GI 162002872
Locus Tag BAB1_1583
Protein Accession YP_414947.2
Other Database IDs UniProtKB-ID: Q2YRT1_BRUA2
UniRef100: UniRef100_B2S726
UniRef90: UniRef90_Q8FZC4
UniRef50: UniRef50_A4QAP0
UniParc: UPI00004F913C
EMBL: AM040264
EMBL-CDS: CAJ11539.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1583
eggNOG: COG0119
HOGENOM: HBG289752
OMA: VERCNQI
ProtClustDB: PRK03739
BioCyc: BMEL359391:BAB1_1583-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1531098
Gene Ending Position 1532765
Gene Strand (Orientation) -
Protein Name 2-isopropylmalate synthase
DNA Sequence 
>gi|82698932:1531098-1532765 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
TCTACTTAGCAATCACACGATTTGCCGCCGACACCACGGCTTCAAGCGAGGCGCTGACGATATTATCGTT
GATGCCGACACCGAACAGCTTGCCGCCGGGATAAACCATCTCGACATAGGAAATGGCCGAAGCATCCGAA
CCCTGCTGGAGCGAATGTTCGGAATAATCGACCACCGACATTTTCACGCCAAGATATTTCGACAGCGCAT
CCACGAAACCATCGATCGGTCCCGTGCCCTTGCCCTCGATAGTCTTGGTGACACCGTTATCGGTGATCTC
AGCCGTCAGGATGCGCCGGCCCTTTTGCTCAGGATCGGGATAGGTGTGGTGATCCACGAACTTGATGCGG
GCATCGGGCTGCGTCACATAGAGTTTCTGGAACTCCTCGTAAATGCGCTTCGATGGCAGCTCCTTGCCCT
CTTCATCCGTAATGTGCTGGATGATTTCGCGGAACTCGACCTGCAGGTTGCGCGGCAGGTTCAGGCCGTA
ATCGGCTTGCAGGATATAGGCGATACCGCCCTTGCCCGATTGCGAATTGATGCGGATGATCGCCTCGTAG
GAGCGGCCCACATCCTGCGGATCGATCGGCAGGTAAGGAACTTCCCAAACCGGCGAATTGGCCGAACGGC
GCGCTTTCATGCCCTTGTTGATCGCATCCTGATGCGAGCCGGAGAAAGCCGTATAGACCAGCTCGCCCAC
ATAAGGATGGCGCTCGGCGATCTTCAACTGGTTGGAATATTCGTAGACTTCCTTCATCCGGTTGATATCG
GTGCAATCCAGCCCGGGGTCGATGCCCTGCGTGTACATGTTGAGCGCCAGCGTCACCACATCCACATTGC
CCGTGCGCTCGCCATTGCCGAACAGCGTGCCTTCCACGCGATCAGCGCCTGCCATCAGGCCCAGTTCAGT
GGCGGCAATGCCCGTGCCGCGGTCATTATGCGGATGCAGCGAGATAATCAGGCTCTCGCGATTGTCCAGA
TTGCGGCACATCCATTCGATCTGGTCGGCATAGACATTCGGCGTGTTCATCTCCACCGTCGAAGGCAGGT
TGACGATCAGCTTGTTGTCCGGCGTCGGCTTCACGATCTCGATCACCGCATTGCAGATTTCCAGCGCCAC
ATCCAGTTCGGTGCCGGTAAAGCTTTCCGGCGAATACTGGAAGCGATAACCGCCGCCAGCCTTGGCAGCC
ATATCCATGATCATCTTGGCCGCATCGGTTGCAATCTGCTTGATGCCGCCGACATCCTTGGCAAACACCA
CGCGGCGCTGCAATTCGCTGGTGGAATTGTAGAAATGGATGATCGGCGTCTTCGCGCCTTCCAGCGCCTC
GAAAGTGCGCGTAATCAATTCAGGACGGCACTGGACCAGAACCTGCAAATCCACGTCGTCAGGAACATCG
CCCTGTTCAATGGCCCAGCGGCAGAAGTCGAAATCCGTCTGCGAAGCGGACGGGAAACCGATCTCGATTT
CCGGGAAACCCATATCGATCAGCAGGCGGAACATGCGCTCCTTGCGGTCATGCCCCATGGGGTCGATCAA
AGCCTGGTTGCCGTCGCGCAGATCGACCGAACACCAGATCGGCGCCTTTTCGATGCGTTTGGAAGACCAA
GTGCGGTCATCGAGCTGCGGCGCAGGGAACGGCGCGTATTTGGTACCGGCAATAGGCA
Protein Sequence 
>gi|162002872|ref|YP_414947.2| 2-isopropylmalate synthase [Brucella abortus 2308] MPIAGTKYAPFPAPQLDDRTWSSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIISLHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTGPIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)(UniProt: Q8FZC4).

CATALYTIC ACTIVITY: Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA(UniProt: Q8FZC4).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1(UniProt: Q8FZC4).

SUBUNIT: Homotetramer (By similarity)(UniProt: Q8FZC4).

SIMILARITY: Belongs to the alpha-IPM synthetase/homocitrate synthase family. LeuA type 2 subfamily(UniProt: Q8FZC4).

MUTATION: leuA is a B. suis gene identified by signature-tagged mutagenesis. It is attenuated inside THP1 macrophage cell line.
COG COG0119E, under E: Amino acid transport and metabolism
References