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ilvD from Brucella melitensis biovar Abortus 2308

Victor ID 1723
Gene Name ilvD from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788763
NCBI Protein GI 82699018
Locus Tag BAB1_0096
Protein Accession YP_413592.1
Other Database IDs UniProtKB-ID: ILVD_BRUA2
UniRef100: UniRef100_B2S7Z5
UniRef90: UniRef90_B2S7Z5
UniRef50: UniRef50_Q5F8G6
UniParc: UPI00004F8E5E
EMBL: AM040264
EMBL-CDS: CAJ10052.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_0096
eggNOG: COG0129
HOGENOM: HBG671001
OMA: KVPCLSK
ProtClustDB: PRK12448
BioCyc: BMEL359391:BAB1_0096-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 101039
Gene Ending Position 102874
Gene Strand (Orientation) -
Protein Name dihydroxy-acid dehydratase
DNA Sequence 
>gi|82698932:101039-102874 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
ATCAATCCGGCAGCTTTCGCACCGCGCCCCTGGCGGCACTTGTCGCCATTGCCGCATAGGCTTTCAGCGC
AGTGGAGATTTTACGCTTGCGTTCCTCTGCCGGTTTCCAGCCTGCTGCATCCTGCTCGGCGCGGCGTTCG
GCAAGTGTGGCGTCGTCAACGGCAAGGTGGATCTTGCGGTTCGGAATGTCGATATCGATGATATCGCCTT
CGCGCACCAGACCGATCGTGCCGCCTTCGGCTGCTTCCGGCGAAACATGGCCGATGGAAAGGCCCGATGA
ACCGCCCGAGAAACGTCCGTCGGTGATAAGTGCGCAGGCTTTGCCGAGGCCCTTCGACTTCAGATAGCTG
GTCGGGTAGAGCATTTCCTGCATTCCGGGGCCGCCGCGCGGGCCTTCATAGCGGATCAGCACGATGTCGC
CCGGCTTGATCTTGCCGTTCAAAATGCCAAGCACAGCCGAATCCTGGCTTTCAAAAATGCGGGCCGGGCC
GGAGAATTTCAGGATGGAATCGTCCACGCCTGCCGTCTTCACGATGCAGCCGTCTTCCGCCAGATTGCCG
TAAAGCACGGCCAAGCCGCCATCCTGGCTGAAAGCGTGTTCCTTTGAGCGGATGACGCCTTTTTCGCGGT
CGGTGTCCACCTTGTCGAAGCGCCGCTCCTGGCTGAAAGCCACCTGCGTCGGCACGCCGCCGGGAGCGGC
GGAATAGAATTTGTGCACCATTTCGCTGTTTGTGCGCGTTACATCCCAATGATCAAGCGCCTTAGCGAGC
GTTTCGCTGTGCACGGTCGGGATGCTGGTGGTCAGCAGGCCCGCATTGTCGAGCTGTCCGAGAATGCCCA
TGATGCCGCCGGCATGATGCACGTCTTCCATGTGAACCGACGACACGGCGGGCGCCACCTTGCACAGCAC
CGGCACGCGGCGCGACAGACGGTCTATATCCGCCATGGTGAAATCGATTTCCGCTTCCTGCGCAGCCGCG
AGCAGATGCAGAACCGTATTGGTCGAACCGCCCATGGCGATATCGAGCGTCATCGCGTTCTCGAAAGCCG
AGAAAGTGGCGATGGAGCGCGGCAGCACACTTTCGTCATCCTGCTCGTAATAGCGGCGGGCGAGATCGAC
GATCAGGTGGCCAGCTTCGACGAAGAGGCGCTTGCGGTCTGCATGGGTGGCGAGCGTCGAACCATTGCCG
GGCAGCGACAGGCCGAGCGCTTCGGTAAGGCAATTCATCGAATTGGCCGTGAACATGCCCGAACACGACC
CACAGGTCGGGCAGGCGGAACGCTCAATCGCCTTCACCTGCTCGTCGGTATAATGGTCGTCGGCCGCCGC
CACCATGGCGTCCACGAGGTCGAGCTTCTTGACAGAATCCTCCCACACGACCTTGCCTGCTTCCATCGGC
CCACCGGAGACGAACACGACCGGAATATTGAGACGCAGCGCCGCCATCAGCATGCCGGGCGTGATCTTGT
CGCAATTGGAGATGCAGACCATCGCATCGGCGCAATGCGCATTGACCATATATTCGACCGAGTCGGCGAT
GAGCTCACGGGAGGGGAGCGAATAGAGCATGCCGTCATGGCCCATGGCGATGCCGTCATCGACCGCAATG
GTATTGAATTCCTTGGCGACGCCGCCCGCACTTTCGATTTCGCGCGCGACGAGCTGGCCGAGATCCTTCA
GATGCACATGGCCGGGCACGAACTGGGTGAACGAGTTCACCACGGCGATAATCGGCTTGCCGAAATCCTC
ATCCTTCATGCCGGTGGCGCGCCATAGGCCGCGCGCGCCGGCCATATTGCGGCCATGGGTGGTTGTACGC
GAACGATAAGGAGGCA
Protein Sequence 
>gi|82699018|ref|YP_413592.1| dihydroxy-acid dehydratase [Brucella abortus 2308] MPPYRSRTTTHGRNMAGARGLWRATGMKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIESAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKVVWEDSVKKLDLVDAMVAAADDHYTDEQVKAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADRKRLFVEAGHLIVDLARRYYEQDDESVLPRSIATFSAFENAMTLDIAMGGSTNTVLHLLAAAQEAEIDFTMADIDRLSRRVPVLCKVAPAVSSVHMEDVHHAGGIMGILGQLDNAGLLTTSIPTVHSETLAKALDHWDVTRTNSEMVHKFYSAAPGGVPTQVAFSQERRFDKVDTDREKGVIRSKEHAFSQDGGLAVLYGNLAEDGCIVKTAGVDDSILKFSGPARIFESQDSAVLGILNGKIKPGDIVLIRYEGPRGGPGMQEMLYPTSYLKSKGLGKACALITDGRFSGGSSGLSIGHVSPEAAEGGTIGLVREGDIIDIDIPNRKIHLAVDDATLAERRAEQDAAGWKPAEERKRKISTALKAYAAMATSAARGAVRKLPD
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O(UniProt: Q8G353).

COFACTOR: Binds 1 4Fe-4S cluster (Potential)(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3(UniProt: Q8G353).

PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3(UniProt: Q8G353).

SIMILARITY: Belongs to the ilvD/edd family(UniProt: Q8G353).

MUTATION: Of those B abortus mutants with mini-Tn5 insertions in genes predicted to be involved in amino acid biosynthesis and transport, only the ilvD mutant, displayed attenuation in both macrophages and mice. The othre two amino acid biosynthesis mutants [trpB::miniTn5 and pheA::miniTn5] displayed wild-type virulence in mice but attenuated inside macrophages. The studies with B abortus ilvD, trpB, and pheA mutants suggest that tryptophan and phenylalanine are available to the brucellae in their intracellular niche but that other amino acids (eg, leucine, isoleucine, or valine) are not (Alcantara et al., 2004).
COG COG0129EG, under E: Amino acid transport and metabolism; G: Carbohydrate transport and metabolism
References
Alcantara et al., 2004: Alcantara RB, Read RD, Valderas MW, Brown TD, Roop RM 2nd. Intact purine biosynthesis pathways are required for wild-type virulence of Brucella abortus 2308 in the BALB/c mouse model. Infection and immunity. 2004; 72(8); 4911-4917. [PubMed: 15271960].