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hisF from Brucella melitensis biovar Abortus 2308

Victor ID 1721
Gene Name hisF from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788754
NCBI Protein GI 82700840
Locus Tag BAB1_2086
Protein Accession YP_415414.1
Other Database IDs UniProtKB-ID: HIS6_BRUA2
UniRef100: UniRef100_B2S984
UniRef90: UniRef90_B2S984
UniRef50: UniRef50_Q12UY6
UniParc: UPI00000DD7A2
EMBL: AM040264
EMBL-CDS: CAJ12042.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_2086
eggNOG: COG0107
HOGENOM: HBG541613
OMA: RVVKGTN
ProtClustDB: PRK02083
BioCyc: BMEL359391:BAB1_2086-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 2021984
Gene Ending Position 2022769
Gene Strand (Orientation) +
Protein Name imidazole glycerol phosphate synthase subunit HisF
DNA Sequence 
>gi|82698932:2021984-2022769 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
CATGACTTTGAAAGCAAGAGTGATTCCCTGTCTCGATGTGAAAGATGGCCGCGTCGTCAAAGGTGTGAAC
TTCGTTGATCTGATCGATGCCGGTGATCCGGTGGAAGCCGCCCGCGCCTATGATGCGGCAGGCGCCGACG
AATTGTGCTTTCTCGACATCACGGCGTCTTCGGACAATCGCGAGACAATTTTCGATGTGGTGGCGCGTAC
AGCCGAACAGTGCTTCATGCCCTTGACCGTTGGTGGCGGGGTTCGCCAGGTGGCCGATATCCGCAAGCTT
CTTTTGGCGGGAGCCGACAAGGTGTCGATCAATACCGCAGCGGTGAAGAACCCGGAATTCGTGGCGGAAG
CCGCCGACAAGTTTGGCAATCAATGCATTGTTGTCGCCATCGATGCGAAAAAGGTTTCCGGCGCGGGTGA
AAATGATCGCTGGGAAATCTTCACCCATGGCGGGCGGCAGCCGACCGGCATCGATGCGGTGGAATTTGCA
CAGAAGGTCGTGGACCTTGGCGCAGGCGAAATCCTGCTCACCTCCATGGACCGCGATGGCACCAAGGCGG
GGTACGACGTGGCGCTGACCCGCGCCGTGGCCGATAGCGTGCGCGCGCCGGTCATCGCATCGGGCGGCGT
CGGCACTCTCGATCATCTGGTGGCGGGCATTCGCGATGGCCATGCGACGGCGGTTCTCGCCGCTTCGATT
TTCCACTTCGGCACCTATACGATCGGCGAGGCCAAGCGCTATATGGCCGAAGCCGGCATTCCGATGCGGC
TCGATCCGGTCCGTTG
Protein Sequence 
>gi|82700840|ref|YP_415414.1| imidazole glycerol phosphate synthase subunit HisF [Brucella abortus 2308] MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVVARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADKFGNQCIVVAIDAKKVSGAGENDRWEIFTHGGRQPTGIDAVEFAQKVVDLGAGEILLTSMDRDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYTIGEAKRYMAEAGIPMRLDPVR
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit (By similarity)(UniProt: Q8FY07).

CATALYTIC ACTIVITY: 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O(UniProt: Q8FY07).

PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5(UniProt: Q8FY07).

SUBUNIT: Heterodimer of hisH and hisF (By similarity)(UniProt: Q8FY07).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: Q8FY07).

SIMILARITY: Belongs to the hisA/hisF family(UniProt: Q8FY07).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis hisF gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0107E, under E: Amino acid transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].