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hfq from Brucella melitensis biovar Abortus 2308

Victor ID 1718
Gene Name hfq from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788748
NCBI Protein GI 82699953
Locus Tag BAB1_1134
Protein Accession YP_414527.1
Other Database IDs UniProtKB-ID: HFQ_BRUA2
UniRef100: UniRef100_B2S5W3
UniRef90: UniRef90_B3PYU7
UniRef50: UniRef50_B3PYU7
UniParc: UPI0000057E52
EMBL: AF154075
EMBL: AM040264
EMBL-CDS: AAD38187.1
EMBL-CDS: CAJ11090.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1134
eggNOG: COG1923
HOGENOM: HBG645207
OMA: PYLNVLR
ProtClustDB: PRK00395
BioCyc: BMEL359391:BAB1_1134-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1102488
Gene Ending Position 1102724
Gene Strand (Orientation) -
Protein Name RNA-binding protein Hfq
DNA Sequence 
>gi|82698932:1102488-1102724 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
GTCAGGCTTCCTCGCCTTCAAACATCTGTACAGGCTGGCTCGGCATGATTGTCGAAATAGCATGCTTGTA
AACAAGCTGCGAATGACCATCACGACGCAGAAGCACGCAGAAATTATCGAAAGACGTTACAATGCCGGTC
AATTTCACGCCATTGATAAGGAAGATCGTTAGAGAAATCTTCTGCTTGCGGACAGAATTCAGAAAGAGGT
CTTGTAGATTTTGCGATCGTTCAGCCA
Protein Sequence 
>gi|82699953|ref|YP_414527.1| RNA-binding protein Hfq [Brucella abortus 2308] MAERSQNLQDLFLNSVRKQKISLTIFLINGVKLTGIVTSFDNFCVLLRRDGHSQLVYKHAISTIMPSQPVQMFEGEEA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: RNA-binding protein that stimulates the elongation of poly(A) tails (By similarity)(UniProt: P0A3G8).

SIMILARITY: Belongs to the hfq family(UniProt: P0A3G8).

MUTATION: hfq encodes for the RNA binding protein host factor I (HF-I). The hfq knock out strain has been showed a reduced growth rate and is unable to utilize glucose as a sole carbon source(Sonnleitner et al., 2003).

hfq is required for the efficient translation of the stationary-phase sigma factor RpoS in many bacteria, and a Brucella abortus hfq mutant displays a phenotype in vitro, which suggests that it has a generalized defect in stationary-phase physiology. The inability of the B. abortus hfq mutant to survive and replicate in a wild-type manner in cultured murine macrophages, and the profound attenuation displayed by this strain and its B melitensis counterpart in experimentally infected animals indicate that stationary -phase physiology plays an essential role in the capacity of the brucellae to establish and maintain long-term intracellular residence in host macrophages (Roop et al., 2003).

In contrast to B abortus 2308, the isogenic hfq and bacA mutants remained in acidic, LAMP-1 phagosomes and failed to initiate intracellular replication (Roop et al., 2003).

A hfq mutant of B abortus was eliminated from mouse spleens more rapidly than the wild type (Roop et al., 2003).
COG COG1923R, under R: General function prediction only
References
Roop et al., 2003: Roop RM 2nd, Gee JM, Robertson GT, Richardson JM, Ng WL, Winkler ME. Brucella stationary-phase gene expression and virulence. Annual review of microbiology. 2003; 57; 57-76. [PubMed: 12730323].
Sonnleitner et al., 2003: Sonnleitner E, Hagens S, Rosenau F, Wilhelm S, Habel A, Jäger KE, Bläsi U. Reduced virulence of a hfq mutant of Pseudomonas aeruginosa O1. Microbial pathogenesis. 2003; 35(5); 217-228. [PubMed: 14521880].