Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

dut from Brucella melitensis biovar Abortus 2308

Victor ID 1713
Gene Name dut from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3788687
NCBI Protein GI 82700463
Locus Tag BAB1_1687
Protein Accession YP_415037.1
Other Database IDs UniProtKB-ID: DUT_BRUA2
UniRef100: UniRef100_B2S7B5
UniRef90: UniRef90_B2S7B5
UniRef50: UniRef50_B2S7B5
UniParc: UPI000012996B
EMBL: AM040264
EMBL-CDS: CAJ11643.1
RefSeq_NT: NC_007618.1
PDB: 3MBQ
PDB: 3MDX
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1687
eggNOG: COG0756
HOGENOM: HBG436079
OMA: LDLRACI
ProtClustDB: PRK00601
BioCyc: BMEL359391:BAB1_1687-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1633233
Gene Ending Position 1633706
Gene Strand (Orientation) -
Protein Name deoxyuridine 5'-triphosphate nucleotidohydrolase
DNA Sequence 
>gi|82698932:1633233-1633706 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
CTCAGGCTGTGCCGGTGGAACCGAACCCGCCCGCGCCGCGGGCTGTTTCACTTATCTTGGCGCGTTCCTC
GATTTTCGGCTGGATGACCGGAGCGAACACGGCCTGTGCGATGCGCATTCCGCGCTCGATGCGGAAATCG
TCATCTCCAAGATTGATCAGCAGGACTTTTACTTCGCCGCGATAATCCGAATCGATCGTGCCGGGAGTGT
TGAGGCAGGTTATGCCATTCTTGAAGGCAAGGCCCGAGCGGGGGCGGATCTGTACTTCATAGCCTTGCGG
GATTTCAAGGATAAGGCCGGTTGGCACCAGCGTGCGGCGGCCGGGCAAGAGCACGATCTGGCGATCTTCA
GCCACGGCGGCGCGCAGGTCCATGCCCGCCGAACCGGCGGTTTCATAGGCGGGAAGATCAAGGCCCTTCG
CATGTTCGAGGCGAATGATGCCGAGCGTTGGCGCGGAAGAGGATGCTGCGGTCA
Protein Sequence 
>gi|82700463|ref|YP_415037.1| deoxyuridine 5'-triphosphate nucleotidohydrolase [Brucella abortus 2308] MTAASSSAPTLGIIRLEHAKGLDLPAYETAGSAGMDLRAAVAEDRQIVLLPGRRTLVPTGLILEIPQGYEVQIRPRSGLAFKNGITCLNTPGTIDSDYRGEVKVLLINLGDDDFRIERGMRIAQAVFAPVIQPKIEERAKISETARGAGGFGSTGTA
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA (By similarity)(UniProt: P64005).

CATALYTIC ACTIVITY: dUTP + H(2)O = dUMP + diphosphate(UniProt: P64005).

PATHWAY: De novo synthesis of thymidylate(UniProt: P64005).

SIMILARITY: Belongs toMUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis dut gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002). the dUTPase family(UniProt: P64005).
COG COG0756F, under F: Nucleotide transport and metabolism
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].