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miaA from Brucella melitensis biovar Abortus 2308

Victor ID 1708
Gene Name miaA from Brucella melitensis biovar Abortus 2308
Sequence Strain (Species/Organism) Brucella melitensis biovar Abortus 2308
NCBI Gene ID 3787973
NCBI Protein GI 82700207
Locus Tag BAB1_1409
Protein Accession YP_414781.1
Other Database IDs UniProtKB-ID: MIAA_BRUA2
UniRef100: UniRef100_B2S6L5
UniRef90: UniRef90_B2S6L5
UniRef50: UniRef50_A9M648
UniParc: UPI00004F90E3
EMBL: AM040264
EMBL-CDS: CAJ11365.1
RefSeq_NT: NC_007618.1
GenomeReviews: AM040264_GR
KEGG: bmf:BAB1_1409
eggNOG: COG0324
HOGENOM: HBG559459
OMA: VNADSMQ
ProtClustDB: PRK00091
BioCyc: BMEL359391:BAB1_1409-MONOMER
Taxonomy ID 359391
Chromosome No I
Gene Starting Position 1360337
Gene Ending Position 1361269
Gene Strand (Orientation) -
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|82698932:1360337-1361269 Brucella melitensis biovar Abortus 2308 chromosome I, complete sequence
TTTATTGCGGATCCCGAAACGAACCGCCCTGAAACACCTCTTCGCCACTGGCATAGACACGCCAGTCTTC
GCCCAACTGATTGCGAAACCAGGTGGACTGGCGCTTGGCATATTGCCGCGTGGCAATGACGGAACGTTCA
ATAGCCTCCTCGCGCGACATGGTTTCTGCAAGAAATGCACTGACTTCCCTGACGCCAATGGCCTTCATGG
CGGGTAGCGCAGGATCGAGATCGAGCGCCAGAAGCGCCCTCACCTCATCGATTGCCCCCTCCGCCCACAT
GGCCGAAAAACGCCGCGCAATACGCTCGCCAAGCCATGCACGATCAGGCCGGAGCACGATCTTTTGCGCC
GATTGGTCATCCACCAGCGCCCTGCCCTTGACCTTTTGCCATTCCAAAAGGGATTTGCCGGTGCTTTCCA
GCACTTCCAGCGCACGCACAATGCGCTGGCTATCGGAGGGCTGCAAGGCAGCCGCGATGGCCGGGTCACG
CACACAAAGGACAGCGTGCAGGGCCTTTGCGCCTTCGGCTTCCATCCGGCCTCGCCAATGGTCGCGCACT
TGCGCCGATACTTCCGGCGTTTGCGAAAGCCCGCCCAGAAGCGCACGAAAATAAAGCCCTGTCCCGCCGA
CGAAGATCGGCACCCGGCCTTGCAGTTCGCAACGCCCCAGAAGTGCTTCGACATCCTCAAACCATTTGCC
GGTGGAATAGGTGCTGGAGGGCGGCACATGGCCGTAAAGAAAATGTTCGGCCTCGGCCAGATCGGCCCTG
GAAGGCCGCGCGGTCAAAAGATCCAGAACGCCATAAACCTGCATGGAATCGGTATTGACGATGAAACCGC
CCGTCGCTTTGGCCATGCGAATGGCAAGTGCCGACTTGCCGCTGGCCGTTGGGCCAGCTATCAGGATCGC
ATTCTTGACTGCATCCTCACTCA
Protein Sequence 
>gi|82700207|ref|YP_414781.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Brucella abortus 2308] MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLAEAEHFLYGHVPPSSTYSTGKWFEDVEALLGRCELQGRVPIFVGGTGLYFRALLGGLSQTPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPALPAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the first step in the biosynthesis of 2-methylthio-N6-(delta(2)-isopentenyl)-adenosine (MS[2]I[6]A) adjacent to the anticodon of several tRNA species (By similarity)(UniProt: Q8CY40).

CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine(UniProt: Q8CY40).

SIMILARITY: Belongs to the IPP transferase family(UniProt: Q8CY40).

MUTATION: A study with miniTn5 mutants of B. suis constitutively expressing gfp indicates that B. suis miaA gene is required for intracellular multiplication in human macrophage THP-1 cells (Kohler et al., 2002).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Kohler et al., 2002: Kohler S, Foulongne V, Ouahrani-Bettache S, Bourg G, Teyssier J, Ramuz M, Liautard JP. The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell. Proceedings of the National Academy of Sciences of the United States of America. 2002 Nov 26; 99(24); 15711-6. [PubMed: 12438693 ].