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miaA from Shigella boydii CDC 3083-94

Victor ID 1658
Gene Name miaA from Shigella boydii CDC 3083-94
Sequence Strain (Species/Organism) Shigella boydii CDC 3083-94
NCBI Gene ID 6269094
NCBI Protein GI 187734140
Locus Tag SbBS512_E4702
Protein Accession YP_001882862.1
Other Database IDs UniProtKB-ID: MIAA_SHIB3
UniRef100: UniRef100_B2TY46
UniRef90: UniRef90_A8AMN0
UniRef50: UniRef50_Q7U347
UniParc: UPI00005F1FD4
EMBL: CP001063
EMBL-CDS: ACD10406.1
RefSeq_NT: NC_010658.1
EnsemblGenome: EBESCG00000072010
EnsemblGenome_TRS: EBESCT00000072963
EnsemblGenome_PRO: EBESCP00000070282
GenomeReviews: CP001063_GR
KEGG: sbc:SbBS512_E4702
HOGENOM: HBG559459
OMA: VNADSMQ
ProtClustDB: PRK00091
Taxonomy ID 344609
Gene Starting Position 4394800
Gene Ending Position 4395750
Gene Strand (Orientation) +
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|187730020:4394800-4395750 Shigella boydii CDC 3083-94 chromosome, complete genome
GATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTGATGGGGCCGACGGCCTCCGGTAAA
ACGGCGTTAGCCATTGAGCTGCGTAAAATTTTACCAGTAGAGTTGATAAGCGTTGATTCTGCCCTTATTT
ACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGTGCCGCACCGATTGCT
GGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCC
GATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGG
AAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTGCGGGCCAGAATTGAGCAACAGGCGGCAGAGCA
AGGTTGGGAGTCATTGCATCGTCAACTTCAGGAGGTAGATCCGGTTGCGGCAGCAAGGATTCATCCAAAT
GATCCACAAAGGCTTTCCCGGGCACTGGAAGTTTTTTTCATTTCGGGTAAAACTTTAACGGAACTGACGC
AAACGTCAGGAGACGCTCTACCGTATCAGGTGCATCAGTTCGCCATCGCCCCGGCGAGCCGTGAACTGCT
CCATCAACGCATTGAGCAGCGTTTTCATCAGATGTTGGCTTCAGGTTTTGAAGCAGAAGTCCGGGCGCTT
TTTGCCCGAGGAGATTTGCATACGGACTTGCCTTCCATTCGTTGCGTGGGTTATCGCCAGATGTGGTCTT
ACCTTGAAGGCGAAATCTCATACGATGAAATGGTTTATCGAGGTGTTTGCGCCACGAGACAGTTGGCGAA
GCGGCAGATAACCTGGCTGCGTGGTTGGGAAGGGGTTCACTGGCTTGACAGTGAAAAACCAGAACAGGCG
CGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTG
Protein Sequence 
>gi|187734140|ref|YP_001882862.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Shigella boydii CDC 3083-94] MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSALIYKGMDIGTAKPNAEELLAVPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARIHPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATRQLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: miaA mutants had reduced ability to invade and spread in MK2 cell monolayers compared to the wildtype (Durand et al., 1997).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Durand et al., 1997: Durand JM, Björk GR, Kuwae A, Yoshikawa M, Sasakawa C. The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes. Journal of bacteriology. 1997; 179(18); 5777-5782. [PubMed: 9294434].