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miaA from Shigella boydii Sb227

Victor ID 1571
Gene Name miaA from Shigella boydii Sb227
Sequence Strain (Species/Organism) Shigella boydii Sb227
NCBI Gene ID 3783268
NCBI Protein GI 82546588
Locus Tag SBO_4285
Protein Accession YP_410535.1
Other Database IDs UniProtKB-ID: MIAA_SHIBS
UniRef100: UniRef100_A7ZV40
UniRef90: UniRef90_A8AMN0
UniRef50: UniRef50_Q7U347
UniParc: UPI000012F0BD
EMBL: CP000036
EMBL-CDS: ABB68707.1
RefSeq_NT: NC_007613.1
EnsemblGenome: EBESCG00000076289
EnsemblGenome_TRS: EBESCT00000077242
EnsemblGenome_PRO: EBESCP00000074389
GenomeReviews: CP000036_GR
KEGG: sbo:SBO_4285
HOGENOM: HBG559459
OMA: VNADSMQ
ProtClustDB: PRK00091
BioCyc: SBOY300268:SBO_4285-MONOMER
Taxonomy ID 300268
Gene Starting Position 4334077
Gene Ending Position 4335027
Gene Strand (Orientation) -
Protein Name tRNA delta(2)-isopentenylpyrophosphate transferase
DNA Sequence 
>gi|82542618:4334077-4335027 Shigella boydii Sb227, complete genome
TTCAGCCTGCGATAGCACCAACAACCTGTAATACTTCGTCACGCGCCTGTTCTGGTTTTTCACTGTCAAG
CCAGTGAACCCCTTCCCAACCACGCAGCCAGGTTATCTGCCGCTTCGCCAACTGTCTCGTGGCGCAAACA
CCTCGATAAACCATTTCATCGTATGAGATTTCGCCTTCAAGGTAAGACCACATCTGGCGATAACCCACGC
AACGAATGGAAGGCAAGTCCGTATGCAAATCTCCTCGGGCAAAAAGCGCCCGGACTTCTGCTTCAAAACC
TGAAGCCAACATCTGATGAAAACGCTGCTCAATGCGTTGATGGAGCAGTTCACGGCTCGCCGGGGCGATG
GCGAACTGATGCACCTGATACGGTAGAGCGTCTCCTGACGTTTGCGTCAGTTCCGTTAAAGTTTTACCCG
AAATGAAAAAAACTTCCAGTGCCCGGGAAAGCCTTTGTGGATCATTTGGATGAATCCTTGCTGCCGCAAC
CGGATCTACCTCCTGAAGTTGACGATGCAATGACTCCCAACCTTGCTCTGCCGCCTGTTGCTCAATTCTG
GCCCGCACTTCCGGGTCTGCCGACGGTAGCGGCGACAACCCTTCCAGCAATGCCTTGAAATACAACATCG
TACCGCCCACTAACAGTGGGATCCGCCCCGCCGCGGTGATATCGGCCATTTCCGCCAGCGCATCGCGGCG
AAAATCAGCAGCCGAGTAAGCCTGCGACGGATCGCGAATATCCAGCAATCGGTGCGGCGCGGCGAGTAAC
TCTTCAGCGTTCGGCTTCGCCGTCCCGATATCCATCCCTTTGTAAATAAGGGCAGAATCAACGCTTATCA
ACTCTACTGGTAAAATTTTACGCAGCTCAATGGCTAACGCCGTTTTACCGGAGGCCGTCGGCCCCATCAA
AAAAATCGCCTTAGGCAGGCTCGCCTTACTGATATCACTCA
Protein Sequence 
>gi|82546588|ref|YP_410535.1| tRNA delta(2)-isopentenylpyrophosphate transferase [Shigella boydii Sb227] MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSALIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIPLLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARIHPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATRQLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: miaA mutants had reduced ability to invade and spread in MK2 cell monolayers compared to the wildtype (Durand et al., 1997).
COG COG0324J, under J: Translation, ribosomal structure and biogenesis
References
Durand et al., 1997: Durand JM, Björk GR, Kuwae A, Yoshikawa M, Sasakawa C. The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes. Journal of bacteriology. 1997; 179(18); 5777-5782. [PubMed: 9294434].