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pyrB from Brucella melitensis bv. 1 str. 16M

Victor ID 1380
Gene Name pyrB from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1198442
NCBI Protein GI 17989015
Locus Tag BMEII0670
Protein Accession NP_541648.1
Other Database IDs UniProtKB-ID: D0B735_BRUME
UniRef100: UniRef100_B2SB57
UniRef90: UniRef90_B2SB57
UniRef50: UniRef50_A4VRI1
UniParc: UPI000005858D
EMBL: GG703779
EMBL-CDS: EEW87427.1
RefSeq_NT: NC_003318.1
OMA: VMMLRVQ
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 704258
Gene Ending Position 705226
Gene Strand (Orientation) -
Protein Name aspartate carbamoyltransferase catalytic subunit
DNA Sequence 
>gi|17988344:704258-705226 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CTCATGCCGGTTCTCCGTTGCCTGGGTTGCGCCGCGGATCGAGCAGAGCCTCCATCACGGCCATGCGCAC
GGCAACGCCCATTTCCACCTGTTGCTGGATGACGCTTTGCGGCCCGTCCGCAACGTCGGAGGCAATCTCG
ACGCCCCGGTTCATTGGCCCCGGATGCATCACAAGTGCATCGGGTTTGGCGAATTTGAGTTTTTCCCGGT
CCAGGCCATAGAAATGGAAATATTCCCGCACGGAAGGCACGAAGGAACCCGCCATGCGCTCGCGCTGGAG
GCGCAGCATCATGACGACATCAGCGTCTTTCAGCCCCTCCTCCATGGAGTTGAAAACTTCCACGCTCATA
TCGGCCATGCCGGCGGGCAGGAGTGTTGAAGGCGCAACCACCCGCACCCGCGCGCCGAGCGCGTTGAGCA
GGAGAATGTTGGAGCGCGCCACGCGCGAATGCAGAACGTCACCGCAGATGGCGACGATCAGATTTTCGAT
CTGGCCCTTGGCGCGGCGGATTGTCAGCGCATCGAGAAGCGCCTGTGTGGGGTGTTCATGCGCGCCGTCG
CCCGCATTGACCACCGAGCAACCCACTTTCTGCGCGAGAAGGGCGGCAGCACCGGCGGAAGCATGGCGGA
TGACGAGAATATCCGGCTGCATGGCGTTCAGCGTCATCGCCGTATCGATGAGCGTTTCGCCTTTCTTGAC
CGATGAATTGCCGACGGACATATTCATCACATCCGCGCCCAGCCGTTTTCCGGCCAGCTCGAACGAAGAT
TGCGTCCGTGTCGATGCTTCAAAGAACAGGTTGATTTGCGTGCGACCGCGCAGCACGGACTTTTTCTTCT
CCGGCTGCCGGGAGACAGCGATTTCCTGATCGGCAAGATCGAGAAGGCAGAGAATGTCCAGGGGCGATAG
CCCCTTGATGCCAAGCAGGTGGCGGTGAGGGAAAAGCGGAGAGACCGTTTGATTTGTCA
Protein Sequence 
>gi|17989015|ref|NP_541648.1| aspartate carbamoyltransferase catalytic subunit [Brucella melitensis bv. 1 str. 16M] MTNQTVSPLFPHRHLLGIKGLSPLDILCLLDLADQEIAVSRQPEKKKSVLRGRTQINLFFEASTRTQSSFELAGKRLGADVMNMSVGNSSVKKGETLIDTAMTLNAMQPDILVIRHASAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGQIENLIVAICGDVLHSRVARSNILLLNALGARVRVVAPSTLLPAGMADMSVEVFNSMEEGLKDADVVMMLRLQRERMAGSFVPSVREYFHFYGLDREKLKFAKPDALVMHPGPMNRGVEIASDVADGPQSVIQQQVEMGVAVRMAVMEALLDPRRNPGNGEPA
Molecule Role Virulence factor
Molecule Role Annotation CATALYTIC ACTIVITY: Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate(UniProt: P65612).

PATHWAY: Nucleotide biosynthesis; UMP biosynthesis; UMP from HCO(3)(-): step 2(UniProt: P65612).

PATHWAY: Context: Pyrimidine biosynthesis(UniProt: P65612).

SIMILARITY: Belongs to the ATCase/OTCase family(UniProt: P65612).

MUTATION: B. abortus pyrB (pyrimidines) gene is essential for intracellular growth in HeLa cells as shown from transposon mutagenesis study (Kim et al., 2003).
COG COG0540F, under F: Nucleotide transport and metabolism
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].