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sodC from Brucella melitensis bv. 1 str. 16M

Victor ID 1373
Gene Name sodC from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1198353
NCBI Protein GI 17988926
Locus Tag BMEII0581
Protein Accession NP_541559.1
Other Database IDs UniProtKB-ID: D0B7C0_BRUME
UniRef100: UniRef100_P66826
UniRef90: UniRef90_P66826
UniRef50: UniRef50_P0AGD2
UniParc: UPI0000058534
EMBL: GG703779
EMBL-CDS: EEW87512.1
RefSeq_NT: NC_003318.1
OMA: GEHAASM
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 614735
Gene Ending Position 615259
Gene Strand (Orientation) +
Protein Name SUPEROXIDE DISMUTASE (CU-ZN)
DNA Sequence 
>gi|17988344:614735-615259 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
AGTGATGAAGTCCTTATTTATTGCATCGACAATGGTGCTTATGGCTTTTCCGGCTTTCGCAGAAAGCACG
ACGGTAAAAATGTATGAGGCGCTGCCGACCGGACCGGGTAAAGAAGTTGGCACCGTGGTCATTTCCGAAG
CCCCGGGCGGGCTGCACTTCAAGGTGAATATGGAAAAGCTGACGCCGGGCTATCATGGCTTTCATGTTCA
CGAAAATCCAAGCTGCGCTCCGGGAGAAAAAGACGGCAAGATCGTACCGGCTCTTGCTGCCGGCGGGCAT
TATGATCCGGGTAATACCCATCACCATTTAGGGCCTGAAGGTGATGGACATATGGGCGATTTGCCACGCC
TGAGCGCCAATGCTGACGGCAAGGTGAGTGAAACCGTTGTCGCTCCACATCTCAAGAAATTGGCGGAAAT
CAAGCAGCGTTCTTTGATGGTCCATGTCGGAGGGGATAATTATTCCGATAAGCCTGAGCCGCTTGGTGGC
GGTGGTGCCCGTTTTGCCTGCGGCGTGATCGAATA
Protein Sequence 
>gi|17988926|ref|NP_541559.1| superoxide dismutase (Cu-Zn) [Brucella melitensis bv. 1 str. 16M] MMKSLFIASTMVLMAFPAFAESTTVKMYEALPTGPGKEVGTVVISEAPGGLHFKVNMEKLTPGYHGFHVHENPSCAPGEKDGKIVPALAAGGHYDPGNTHHHLGPEGDGHMGDLPRLSANADGKVSETVVAPHLKKLAEIKQRSLMVHVGGDNYSDKPEPLGGGGARFACGVIE
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems (By similarity)(UniProt: P66827).

CATALYTIC ACTIVITY: 2 superoxide + 2 H(+) = O(2) + H(2)O(2)(UniProt: P66827).

COFACTOR: Binds 1 copper ion per subunit (By similarity)(UniProt: P66827).

COFACTOR: Binds 1 zinc ion per subunit (By similarity)(UniProt: P66827).

SUBUNIT: Homodimer (By similarity)(UniProt: P66827).

SUBCELLULAR LOCATION: Periplasmic (By similarity)(UniProt: P66827).

SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family(UniProt: P66827).

IMMUNOGENICITY: Induces antigen-specific Th1 immune response, as indicated by the specific induction of serum IgG2a, but not IgG1, antibodies and by the secretion of IFN-γ, but not IL-4, by the Cu/Zn SOD-stimulated splenocytes. Has been used for vaccine development (Vemulapalli et al., 2000; Vemulapalli et al., 2000; Vemulapalli et al., 2000).

MUTATION: An isogenic sodC mutant constructed from B abortus 2308 by gene replacement exhibited much greater susceptibility to killing by exogenous O(2)(-) than the parental 2308 strain, supporting a role for SodC in protecting this bacterium from O(2)(-) stress. The B abortus sodC mutant was much more sensitive to killing by cultured resident peritoneal macrophages from C57BL6J mice than 2308, and its attenuation in cultured murine macrophages was enhanced when these phagocytes were treated with gamma interferon. The attenuation of the B abortus sodC mutant in both resting and IFN-gamma -activated macrophages was alleviated in the presence of the NADPH oxidase inhibitor apocynin. Consistently, the B abortus sodC mutant also displayed significant attenuation in infected C57BL6J mice compared to the parental strain. These findings suggest that SodC protects B abortus 2308 from the respiratory burst of host macrophages (Vemulapalli et al., 2000).
COG COG2032P, under P: Inorganic ion transport and metabolism
References
Vemulapalli et al., 2000: Vemulapalli R, He Y, Cravero S, Sriranganathan N, Boyle SM, Schurig GG. Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51. Infection and immunity. 2000; 68(6); 3286-3289. [PubMed: 10816475].