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eryB from Brucella melitensis bv. 1 str. 16M

Victor ID 1365
Gene Name eryB from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1198201
NCBI Protein GI 17988774
Locus Tag BMEII0429
Protein Accession NP_541407.1
Other Database IDs UniProtKB-ID: D0B7R6_BRUME
UniRef100: UniRef100_D0B7R6
UniRef90: UniRef90_B2SAH0
UniRef50: UniRef50_B5ZD29
UniParc: UPI000005849C
EMBL: GG703779
EMBL-CDS: EEW87658.1
RefSeq_NT: NC_003318.1
OMA: ILEEWAN
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 446621
Gene Ending Position 448129
Gene Strand (Orientation) -
Protein Name glycerol-3-phosphate dehydrogenase
DNA Sequence 
>gi|17988344:446621-448129 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CTCAAGCAACTAGATTGGCGTTGTCGAACCATTCCACGAAAGCGGCGCGTTCGGCTTCGGTCAAATGCAG
GTAATGCTTGGTGCGGCGATAGAGAATGTCTTCTGCCGTCTTTGCCCATTCTCTGGCCACCAGATAGCGC
ACTTCCGCCTCATGGAAATCGCCGCCGAAGTGACGGCCAAGCCCTTCGAGGTTCTGCGCGCCAGCCACCA
CATCCTTTGTGCGCGCACCATAGAGCCGTCCATAATGATGAACGAGTGTGCGCGGCATCCATGGATAGGT
GTCGCGTAAGGTATTGGCGAAGGTTTCATAATCGGCATTGGCAATCTCGCCGCCGGGCAGCGGCGCGTCG
TGGGTCCAGTCGCCGCCCATTTGCGGGAAAATGTGCTTGAGCCGATGCACCCCGCGCTCCGCCAGTTCGC
GGAAGGTGGTGATCTTGCCGCCAAAGACGTTGAGCAGCGGTGCACCGCCGGTTTCGTCCAGATCGAACAC
GTAATCGCGGGTGACGGCGGAAGGATTGCCCTTGCCGTCGTCAAACAGCGGGCGCACGCCGGAGAAGGAG
TGCAGCACGTCTTCGCGCCGGAGCTTTTCCTTGAAATAGCGGTTTACCGCGGTGATGAGATAGTCGATTT
CCTTCTCATCCGCCGCAACGTCTTCCGCCCGGCCTTCATAGGCAATGTCGGTGGTGCCGATCAGCGCCTT
GTCACCTTCATATGGATTGATGAAGATCACGCGCTTGTCGTGGTTCTGAACGAGATAGGCGTTTGCGCCC
GACCAGAATTTCGGAACGATGATGTGGCTGCCCTTGACGAGGCGCACATTGCGCGACGAGGTGGAGGCAG
CGACATTGTGGATGACGTCCGTGACCCATGGTCCGGCGCAATTGACGATGCAGCGGGCGCGGAAGGTGTG
GGTTTCGCCGGTATCGCTGTTTCTGGTTTCCACGATCCAGCCGCCATTTTCGCGGCGTGCGGAGACGACC
GGTGTACGGGTGAGAATGGTCGCGCCCTTTTCGGCGGCACCCACCGCATTGAGCGCCACGAGGCGCGCAT
CGTCCACCCAGCAATCGGAATATTCAAAACCCTTGGTGTACTGGTCGAGGATCGGCGTGCCTTCCGGGTC
GCGCTTCAGATCGAGCGTACGCGTGCCGGGAAGTTTTTTGCGCCCGCCCAGATGGTCATAGAGAAACAGG
CCAAGCCGCACCAGCCAGGCCGGGCGATCCTGCGGGCTGTGCGGCAGCACGAAGCGCATCGGCCAGATGA
TATGGGGTGCCGCATTCAAAAGCACTTCGCGCTCGATCAGCGCTTCGCGCACAAGGCGGAACTCATAATA
TTCGAGATAGCGCAAACCGCCATGCACCAGCTTGCCGGAGCGCGATGATGTGCCTTGCGCCAGATCGTCC
TTTTCTGCCAGCACCACCTTGAGGCCGCGCCCGGCCGCGTCGCGGGCCACGCCCGCACCGTTGATGCCGC
CGCCAATTACAAACAGGTCGCAGGTTTCCGGTTCAGCCA
Protein Sequence 
>gi|17988774|ref|NP_541407.1| glycerol-3-phosphate dehydrogenase [Brucella melitensis bv. 1 str. 16M] MAEPETCDLFVIGGGINGAGVARDAAGRGLKVVLAEKDDLAQGTSSRSGKLVHGGLRYLEYYEFRLVREALIEREVLLNAAPHIIWPMRFVLPHSPQDRPAWLVRLGLFLYDHLGGRKKLPGTRTLDLKRDPEGTPILDQYTKGFEYSDCWVDDARLVALNAVGAAEKGATILTRTPVVSARRENGGWIVETRNSDTGETHTFRARCIVNCAGPWVTDVIHNVAASTSSRNVRLVKGSHIIVPKFWSGANAYLVQNHDKRVIFINPYEGDKALIGTTDIAYEGRAEDVAADEKEIDYLITAVNRYFKEKLRREDVLHSFSGVRPLFDDGKGNPSAVTRDYVFDLDETGGAPLLNVFGGKITTFRELAERGVHRLKHIFPQMGGDWTHDAPLPGGEIANADYETFANTLRDTYPWMPRTLVHHYGRLYGARTKDVVAGAQNLEGLGRHFGGDFHEAEVRYLVAREWAKTAEDILYRRTKHYLHLTEAERAAFVEWFDNANLVA
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: EryB is an erythritol phosphate dehydrogenase. The vaccine strain B abortus B19 is the only known B abortus isolate whose growth is inhibited by erythritol. The B abortus B19 strain is an eryCD double mutant. The defect in B19 was complemented in trans by plasmids containing the complete ery region and by plasmids with Tn1725 insertions in eryA, eryB and eryD. Plasmids with Tn1725 insertions in eryC were the only ones that failed to complement the Ery phenotype of B19 (Sangari et al., 2000).

The B. suis eryB mutant by Tn5 transposon mutagenesis was attenuated in the human macrophage -like THP-1 cells. This mutant is sensitive to erythritol and mimics the erythritol sensitive response of the B19 strain (Burkhardt et al., 2005).
COG COG0578C, under C: Energy production and conversion
References
Burkhardt et al., 2005: Burkhardt S, Jiménez de Bagüés MP, Liautard JP, Köhler S. Analysis of the behavior of eryC mutants of Brucella suis attenuated in macrophages. Infection and immunity. 2005; 73(10); 6782-6790. [PubMed: 16177356].
Sangari et al., 2000: Sangari FJ, Agüero J, García-Lobo JM. The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus. Microbiology (Reading, England). 2000; 146 ( Pt 2); 487-495. [PubMed: 10708387].