Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

eryC from Brucella melitensis bv. 1 str. 16M

Victor ID 1364
Gene Name eryC from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1198200
NCBI Protein GI 17988773
Locus Tag BMEII0428
Protein Accession NP_541406.1
Other Database IDs UniProtKB-ID: D0B7R7_BRUME
UniRef100: UniRef100_A5VVE3
UniRef90: UniRef90_A5VVE3
UniRef50: UniRef50_A6X312
UniParc: UPI000005849B
EMBL: GG703779
EMBL-CDS: EEW87659.1
RefSeq_NT: NC_003318.1
OMA: HFGHPDA
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 445681
Gene Ending Position 446613
Gene Strand (Orientation) -
Protein Name D-erythrulose 4-phosphate dehydrogenase
DNA Sequence 
>gi|17988344:445681-446613 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CCTATATCTTCAAGTCCTTAGCGCCGGTGTCAATGTGCGGCGCCCAGAAAGCCACACTTTCTGCAATCTG
TGGAATGACTTCACGGTCGTTCGGCTCGCGCTCCTTGAACGAAAGTTCAAGGCAGATTTCATTATCCACC
GCGCCGCCTTCGGCAAAGGCTTTCAAAAGCGGTTCCGGCTGGATGCGGCCCTTGGCATTGAACGCGGCTG
TGAAAGGACGATGCCCGCCCTTGTCCATCAGGCTTTGCTTGATATGGATGATGGGCGAGACTTTCGGCAC
GGTGCGGGCCCATGCGTAAGGATCGTAGTCGTCCGGGTTAGCGGATGTCACGTCACCATGGTCGATATCG
GCCATCATCCACATGGGGATCGCCATGTTAGCGGCGGTGAGCCGATCCTGAAGCTTCATGCATTCGGCAA
TCGTCTCGCCAAATTCGCGCCCGATGCTCATCGGCTCCCAGAACACATAGTCGAGGCCCGCACCTGCCGC
ATGTTCGGCCACCTCGGCCCAGCAGTCGATGGCGATCTTGATAAGTTCTTCGCGGCGCGCCGGATCATCG
AAATCCTTATAGGTGAAGATTGCAAACTGCGTACCGACGGACTTGCCGCCAAGATCGCCGATAATATCGG
CAAAGGTCTTGAACCAGTCCACATAATAGCGGCGCACGTCCCGGTCGGGATGGCCAAAATGGTTGAGGCG
GCCATAGGGGCCGGTCATGCCGGAGGTGACGCGGACGCCGGTGCGCTGCAAGGCCCTGTCCATGTCGCGG
GTGAGGCGGCGGATGGTCGAGGCCTGCCAGCTTGGATTGATGAACTCATGGGTAAGCTGGAGGTCACGCA
GGCGCAGGTCGCGGGCAACCGTTTCGATCAGGTCGTCCGGCTCGGCAAAGCGGTTGACCAGCGGATTGGT
GTTGAGCGAAAGCGTCAGGGCCA
Protein Sequence 
>gi|17988773|ref|NP_541406.1| D-erythrulose 4-phosphate dehydrogenase [Brucella melitensis bv. 1 str. 16M] MALTLSLNTNPLVNRFAEPDDLIETVARDLRLRDLQLTHEFINPSWQASTIRRLTRDMDRALQRTGVRVTSGMTGPYGRLNHFGHPDRDVRRYYVDWFKTFADIIGDLGGKSVGTQFAIFTYKDFDDPARREELIKIAIDCWAEVAEHAAGAGLDYVFWEPMSIGREFGETIAECMKLQDRLTAANMAIPMWMMADIDHGDVTSANPDDYDPYAWARTVPKVSPIIHIKQSLMDKGGHRPFTAAFNAKGRIQPEPLLKAFAEGGAVDNEICLELSFKEREPNDREVIPQIAESVAFWAPHIDTGAKDLKI
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: The eryC gene encodes for enzyme Derythrulose-1-phosphate dehydrogenase. The vaccine strain B abortus B19 is the only known B abortus isolate whose growth is inhibited by erythritol. The B abortus B19 strain is an eryCD double mutant. The defect in B19 was complemented in trans by plasmids containing the complete ery region and by plasmids with Tn1725 insertions in eryA, eryB and eryD. Plasmids with Tn1725 insertions in eryC were the only ones that failed to complement the Ery phenotype of B19 (Sangari et al., 2000).

Allelic exchange mutants in eryC of Brucella suis were erythritol sensitive in vitro with a MIC of 1 to 5 mM of erythritol. Their multiplication in macrophage-like cells was 50 to 90- fold reduced , but complementation of the mutant restored wild-type levels of intracellular multiplication and the capacity to use erythritol as a sole carbon source. In vivo, the eryC mutant colonized the spleens of infected BALBc mice to a significantly lower extent than the wild type and the complemented strain. Interestingly, eryC mutants that were in addition spontaneously erythritol tolerant nevertheless exhibited wild-type-like intramacrophagic and intramurine replication. In conclusion, erythritol was not an essential carbon source for the pathogen in the macrophage host cell but that the inactivation of the eryC gene significantly reduced the intramacrophagic and intramurine fitness of B suis (Burkhardt et al., 2005).
References
Burkhardt et al., 2005: Burkhardt S, Jiménez de Bagüés MP, Liautard JP, Köhler S. Analysis of the behavior of eryC mutants of Brucella suis attenuated in macrophages. Infection and immunity. 2005; 73(10); 6782-6790. [PubMed: 16177356].
Sangari et al., 2000: Sangari FJ, Agüero J, García-Lobo JM. The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus. Microbiology (Reading, England). 2000; 146 ( Pt 2); 487-495. [PubMed: 10708387].