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znuA from Brucella melitensis bv. 1 str. 16M

Victor ID 1354
Gene Name znuA from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197949
NCBI Protein GI 17988522
Locus Tag BMEII0178
Genbank Accession AE008918
Protein Accession NP_541155
Other Database IDs UniProtKB-ID: ZNUA_BRUME
UniRef100: UniRef100_Q8YDJ7
UniRef90: UniRef90_Q2YJH5
UniRef50: UniRef50_Q2YJH5
UniParc: UPI00000DDB62
EMBL: AE008918
EMBL-CDS: AAL53419.1
RefSeq_NT: NC_003318.1
GenomeReviews: AE008918_GR
KEGG: bme:BMEII0178
HOGENOM: HBG731008
OMA: AYQYFEH
ProtClustDB: CLSK886373
BioCyc: BMEL224914:BMEII0178-MONOMER
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 185054
Gene Ending Position 186040
Gene Strand (Orientation) +
Protein Name high-affinity zinc uptake system protein ZNUA
Protein pI 6.17
Protein Weight 33259.01
Protein Length 328
DNA Sequence 
>gi|17988344:185054-186040 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CTTGTTTCTTGCTTCTGCCTTTCTGGCTGGCTTTTGCGGTTCTTCCCTTGCAGGCGAACGTGAGGGCGTG
GTTGTTTCGATCAAGCCGCTTCACTCGATTGTTTCCGCCGTCATGCAGGGCGTGGGTAAGCCGAAGCTGA
TCGTTCAGGGGGCTGGGTCCGAGCATGTTTATAGTCTGAAACCCTCCGATGCCGAGGCTATCGAGCACGC
CAAGGTGATTTTCTGGGCCGGGCCATCCATGGAAACCTTTCTCGACAAGCCCATTGATACGCTGGGCGAG
GGCGCGAAAGTGGTGGCTCTTGGCGATGCGAAAGGGTTGACGAAACTAAAATTCCGCGAGGGTGGGCCGT
TCGAGGCGCACGATCATGGCCATGGGGGGAGCCATGAGGAAGAGCATGACGCACATGGTTCAGGCGATCA
CGACCATGCGGCCGAAGTCGCAGAAGAAGGCCATGAACACCACCATCACGGCGAATATGATCTGCATTTC
TGGCTTGACCCGCAAAATGGCAAGATTCTGGCTGCCGATATCGCCAAAACGCTTGGCGAGAGCGACCCGG
AACACGCCGCGCAATATGAAAAGAATGCCAAGGCCTATGGAGAAAAACTCGATGCCTTGACCAGGGAAGT
GGCTGCTGAACTGAAGCCGGTCAAGGACAAGCCCTTCATCGTCTTTCATGATGCCTATCAATATTTCGAG
AATCGCTTCGGCATGAAAGCAGCCGGCTCCATCACGGTCAGCCCGGAAAAAGCGCCGGGTGCGGCGCGTA
TCCAGCAAATTCACGACAAGATAAAGTCACTCGGTGCAACCTGCGTTTTCTCAGAGCCGCAATTTGAGCC
GAAACTGGTGAAGACGGTGGTTGACGGAACGAAAGCCCGGACAGGTGTTCTTGATCCTCTTGGCGCAGAA
CTGAAGGACGGCCCTGATCTTTATCCGCAACTCATCCGCAATCTGGCCAATTCGCTGAAAGACTGCCTGT
CGAAATA
Protein Sequence 
>gi|17988522|ref|NP_541155.1| high-affinity zinc uptake system protein ZNUA [Brucella melitensis bv. 1 str. 16M]
MFLASAFLAGFCGSSLAGEREGVVVSIKPLHSIVSAVMQGVGKPKLIVQGAGSEHVYSLKPSDAEAIEHA
KVIFWAGPSMETFLDKPIDTLGEGAKVVALGDAKGLTKLKFREGGPFEAHDHGHGGSHEEEHDAHGSGDH
DHAAEVAEEGHEHHHHGEYDLHFWLDPQNGKILAADIAKTLGESDPEHAAQYEKNAKAYGEKLDALTREV
AAELKPVKDKPFIVFHDAYQYFENRFGMKAAGSITVSPEKAPGAARIQQIHDKIKSLGATCVFSEPQFEP
KLVKTVVDGTKARTGVLDPLGAELKDGPDLYPQLIRNLANSLKDCLSK
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: Brucella abortus znuA mutant via mini-Tn5Km2 transposon mutagenesis has intracellular growth defect inside HeLa cells (Kim et al., 2003). High-affinity zinc uptake system protein mutant (znuA mutant) showed reduced growth in zinc chelated medium, and failed to replicate in HeLa cells and mouse bone marrow-derived macrophages. Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. Bacterial internalization into HeLa cells and macrophages and co-localization with either late endosomes or lysosomes of znuA mutant were not different from those of wild-type strain. These results suggest that znuA does not contribute to intracellular trafficking of B abortus, but contributes to utilization of zinc required for intracellular growth (Kim et al., 2004).
COG COG4531P, under P: Inorganic ion transport and metabolism
References
Kim et al., 2003: Kim S, Watarai M, Kondo Y, Erdenebaatar J, Makino S, Shirahata T. Isolation and characterization of mini-Tn5Km2 insertion mutants of Brucella abortus deficient in internalization and intracellular growth in HeLa cells. Infection and immunity. 2003; 71(6); 3020-3027. [PubMed: 12761078].
Kim et al., 2004: Kim S, Watanabe K, Shirahata T, Watarai M. Zinc uptake system (znuA locus) of Brucella abortus is essential for intracellular survival and virulence in mice. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2004; 66(9); 1059-1063. [PubMed: 15472468].