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virb9 from Brucella melitensis bv. 1 str. 16M |
| Victor ID |
1335 |
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Gene Name |
virb9 from Brucella melitensis bv. 1 str. 16M |
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Sequence Strain (Species/Organism) |
Brucella melitensis bv. 1 str. 16M |
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NCBI Gene ID |
1197804
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NCBI Protein GI |
17988377
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Locus Tag |
BMEII0033 |
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Protein Accession |
NP_541010.1 |
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Other Database IDs |
UniProtKB-ID: VIRB9_BRUME
UniRef100: UniRef100_Q8YDZ1
UniRef90: UniRef90_Q2YJ79
UniRef50: UniRef50_Q2YJ79
UniParc: UPI0001529A7B
EMBL: AE008918
EMBL-CDS: AAL53274.1
RefSeq_NT: NC_003318.1
GenomeReviews: AE008918_GR
KEGG: bme:BMEII0033
NMPDR: fig|224914.1.peg.2092
HOGENOM: HBG751000
ProtClustDB: CLSK884262
BioCyc: BMEL224914:BMEII0033-MONOMER
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Taxonomy ID |
224914
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Chromosome No |
II |
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Gene Starting Position |
32320 |
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Gene Ending Position |
33063 |
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Gene Strand (Orientation) |
+ |
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Protein Name |
CHANNEL PROTEIN VIRB9 HOMOLOG |
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DNA Sequence |
>gi|17988344:32320-33063 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CGTGCTGGTTCGGGCCCTGCCCGGCGTTGGCGCACGCATCGTGTTTGCGCCGGGCGAGAATATTGAGGAT
GTCGCCTCAGGATTTACGCAAGGCTGGGAGTTCAAGGCGAGCCACAACATCCTGTATCTCAAGGCCCGCT
CCATGACGCTCTCCCACTCGAACCAATCGATCGACATGGCCCCGGAACCGGGCAAGTGGGACACAAACCT
GATGGTCACGACAGATCAGCGCATGTACGATTTCGATTTGCGCTTGATGCCCGGCCGCAACAATCAGCGT
GTTGCCTATCGTGTTCAGTTCCGCTACCCGGCGGCAGCGGCAGCGGCGGCAGTAGCAGCCGCGCAAAAGC
GAGTGGTACAGGCCCGCATGAATGCCAGACCGTCACCGGTAAACTGGAACTACACGATGCAGGTCGGCAC
TAATTCGGCCAGCATCGCGCCCACTCTTGCCTATGACGATGGGCGGTTCACATATTTGCGCTTTCCGAAC
AACAGAGACTTTCCGGCCGCTTTCCTTGTCGCCGAAGACAAGTCGGAAAGCATCGTGAACAGCCATATAG
ACCCGTCAGCGCCGGACATTCTGGTCCTGCATCGGGTCGCAAAGCAAATGGTGCTGCGCCTCGGTAACAA
GGTGATTGGCATCTACAACGAATCCTTCAATCCTGATGGCGTTCCCGCACGCGACGGAACCACAGTGCCA
CGCGTCAAGCGCGTCATCAAATCGCCCGGGGAGAACCTGCAATG
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Protein Sequence |
>gi|17988377|ref|NP_541010.1| channel protein VIRB9-like protein [Brucella melitensis bv. 1 str. 16M] MLVRALPGVGARIVFAPGENIEDVASGFTQGWEFKASHNILYLKARSMTLSHSNQSIDMAPEPGKWDTNLMVTTDQRMYDFDLRLMPGRNNQRVAYRVQFRYPAAAAAAAVAAAQKRVVQARMNARPSPVNWNYTMQVGTNSASIAPTLAYDDGRFTYLRFPNNRDFPAAFLVAEDKSESIVNSHIDPSAPDILVLHRVAKQMVLRLGNKVIGIYNESFNPDGVPARDGTTVPRVKRVIKSPGENLQ
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
MUTATION: Uptake in the presence or absence of Ca2 and Mg2 did not influence the subsequent intracellular survival of wild-type Brucella, whereas the decrease in the number of surviving virB9 mutant cells was delayed in the absence of Ca2 and Mg2. Possibly two types of adhesion molecules promoted uptake of Brucella, one being Ca2 and Mg2 dependent and the other not, and that both types participate in the uptake of wild-type bacteria but only the latter type participates in the uptake of the virB9 mutant (Rittig et al., 2001).
Four independent mutants in virB5, virB9 or virB10 were highly attenuated in an in vitro infection model with human macrophages (O'Callaghan et al., 1999).
The intracellular fate of three virB mutants (virB2, virB4 and virB9) in HeLa cells by immunofluorescence was examined. The three VirB proteins are not necessary for penetration and the inhibition of phago-lysosomal fusion within non-professional phagocytes. Rather, the virB mutants are unable to reach the replicative niche and reside in a membrane -bound vacuole expressing the late endosomal marker, LAMP1, and the sec61beta protein from the ER membrane, proteins that are present in autophagic vesicles originating from the ER (Delrue et al., 2001).
Attenuated non-polar virB2, virB4, virB8, virB9 and virB10 Brucella mutants are capable of penetrating cells as the same rate as the virulent wild-type Brucella, transit through EEA1 -positive early compartments and then localize in LAMP1-positive compartments at early times of infection (Gorvel and Moreno, 2002). |
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COG
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COG3504U, under U: Intracellular trafficking, secretion, and vesicular transport |
| References |
Delrue et al., 2001: Delrue RM, Martinez-Lorenzo M, Lestrate P, Danese I, Bielarz V, Mertens P, De Bolle X, Tibor A, Gorvel JP, Letesson JJ. Identification of Brucella spp. genes involved in intracellular trafficking. Cellular microbiology. 2001; 3(7); 487-497. [PubMed: 11437834].
Gorvel and Moreno, 2002: Gorvel JP, Moreno E. Brucella intracellular life: from invasion to intracellular replication. Veterinary microbiology. 2002; 90(1-4); 281-297. [PubMed: 12414149].
O'Callaghan et al., 1999: O'Callaghan D, Cazevieille C, Allardet-Servent A, Boschiroli ML, Bourg G, Foulongne V, Frutos P, Kulakov Y, Ramuz M. A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis. Molecular microbiology. 1999; 33(6); 1210-1220. [PubMed: 10510235].
Rittig et al., 2001: Rittig MG, Alvarez-Martinez MT, Porte F, Liautard JP, Rouot B. Intracellular survival of Brucella spp. in human monocytes involves conventional uptake but special phagosomes. Infection and immunity. 2001; 69(6); 3995-4006. [PubMed: 11349069].
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