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virb4 from Brucella melitensis bv. 1 str. 16M |
| Victor ID |
1331 |
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Gene Name |
virb4 from Brucella melitensis bv. 1 str. 16M |
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Sequence Strain (Species/Organism) |
Brucella melitensis bv. 1 str. 16M |
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NCBI Gene ID |
1197799
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NCBI Protein GI |
17988372
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Locus Tag |
BMEII0028 |
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Protein Accession |
NP_541005.1 |
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Other Database IDs |
UniProtKB-ID: D0B5R8_BRUME
UniRef100: UniRef100_Q8YDZ4
UniRef90: UniRef90_Q2YIT8
UniRef50: UniRef50_Q2YIT8
UniParc: UPI000005830B
EMBL: GG703779
EMBL-CDS: EEW86960.1
RefSeq_NT: NC_003318.1
OMA: DYASHTE
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Taxonomy ID |
224914
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Chromosome No |
II |
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Gene Starting Position |
26694 |
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Gene Ending Position |
29189 |
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Gene Strand (Orientation) |
+ |
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Protein Name |
ATPASE VIRB4 HOMOLOG |
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DNA Sequence |
>gi|17988344:26694-29189 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
AATGGGCGCTCAATCCAAATACGCGCAACAGCTCAATAATGAACGGTCACTTGCACCGTTCATTCCCTTC
AGGAGCCAGGTTGGCCCGACCACCGTGATAACGCGTGACGGTGATTTCGTGCGCACATGGCGCATCGCGG
GCCTGGCTTTCGAGACGCAGGACAAGGAGAAACTGTTGATCCGCAAGGACCAGCTTAACACCTTGTTCCG
TGCCATCGCCTCAAACAATGTGGCGCTGTGGTCCCACAACGTACGGCGCAGAACCTGGGATCACTTGAAA
TCCTTCTTTTCCAATCCGTTCTGCGACGCTCTCGACAAGAAGTATTACGGATCGTTTTCCGGCTACCGAA
TGATGTCAAACGAACTGTATCTGACGGTTATTTACCGGCCAGTGCCGGCGAAGATCAGCAGGTTGTTCAA
CGTGGCCGTTCATCGTTCGCATGCGGAAATCTTACAGGAGCAGAAGCTTGCCATCCGCAAGCTGGACGAA
ATCGGCAATCAAATTGAAACCAGCTTGCGTCGCTACGGTGGCGATGACGGGCGTGGCATCGAAGTGCTGA
GCACCTATGAGGACAAACACGGCGCACTGTGCTCACAGCAGCTTGAATTTTATAACTTCCTTCTCTCAGG
CGAATGGCAGAAGGTGCGCGTGCCATCCTGCCCACTGGATGAATATCTGGGCACCGGGTGGGTGTATGCT
GGCACTGAAACCATCGAGATACGCACCGCGAGCGCGACGCGCTATGCGCGTGGCATCGATTTCAAGGACT
ATGCGAACCACACCGAGCCGGGCATTTTGAACGGCCTGATGTATAGCGATTACGAGTACGTCATCACACA
ATCCTTCAGCTTCATGACAAAGCGGGATGGCAAGGAGTTCCTGACACGGCAAAAGCAGCGGCTCCAAAAT
ACCGAAGACGGCAGCGCATCCCAGATCATGGAAATGGACATTGCCATCGACCAGCTCGGCCGGGGCGATT
TCGTCATGGGTGAATATCATTATTCGCTGCTGGTATTTGCCGAAGACATGGAGACGGTGCGCCACAACAC
ATCACATGCGATGAACATCCTGCAAGACAATGGTTTTCTGGCGACGGTGATCGCCACGGCCACGGATGCG
GCGTTCTATGCACAACTGCCATGTAACTGGCGGTATCGGCCGCGCGTGGCGGGCCTGACCTCGCTCAACT
TCGCCGGTCTGTCATGTTTCCATAATTTCCGCGCAGGCAAGCGGGATGGCAACCCCTGGGGGCAGGCATT
GACGCTGCTCAAAACCCCTTCCGGACAGCCCGCCTACCTGAACTTCCACTATTCAAAAGGCGACGAAGAC
AACTTCGACAAGAAGCTTCTGGGCAATACCCGCATCATCGGCCAGTCCGGCGCTGGTAAAACTGTCCTGA
TGAACTTCTGCCTCGCGCAAGCGCAGAAGTATTTGCACAATGCTCCGATGGGTATGTGCAACGTCTTTTT
CGACAAGGATCAAGGCGCGAAAGGGACTATTCTTGCCATTGGCGGCAAATACCTTGCCATCAGGAACGGC
GAACCCACCGGGTTCAACCCATTCCAGATGGAGCCAACAGCGGGGAACATCCTGTTCCTTGAAAAACTCG
TACAGGTACTGGTATCGCGTGATGGCCAGCACGTGACCACAACGGACGAAAGCCGGATCAGTCATGCCAT
ACGTACGGTCATGCGCATGCGCCCGGAGCTGCGGCGTCTGTCGACGGTGCTGCAAAACGTTACCGAAGGC
AGCGACCGCCAAGATCGTGAAAACAGCGTGGCGAAGCGGCTCGCGAAGTGGTGTTTTGATGACGGCACCG
GCAAGCGGGGCACATTCTGGTGGGTGCTGGATTGCCCGCAAGACCAGATCGACTTCAACACGCATAGCAA
TTACGGTTTCGATGGCACCGACTTCCTCGACAACGCCGACGTGCGTACGCCGATTTCCATGTACCTGCTG
CATCGTATGGAATTGGCTATCGACGGGCGTCGTTTCATCTACTGGATGGACGAGGCGTGGAAGTGGGTAG
ACGACGAAGCATTCAGCGAGTTCGCCAATAACAAGCAGCTTACCATCCGCAAGCAAAACGGCCTGGGCGT
GTTTGCCACGCAAATGCCCTCCAGCCTGCTCAACTCCAAAGTCGCGTCGGCCCTGGTGCAACAGGTGGCG
ACGGAAATCTATCTGCCGAATCCGAAAGCCGATTATCACGAGTACACGGACGGCTTCAAGGTGACGAACG
AGGAGTTCGACATCATCCGCAGTATGAGCGAGGAAAGCCGCATGTTCCTGGTCAAGCAGGGGCATCATTC
GATGATTTGCCGCCTGGAGCTTAATGGCTTTGATGACGAACTGGCGATCCTCTCAGGCAGCTCGGACAAC
AACGAGTTGCTGGACCAGGTCATTGCCGAAGTCGGGGACGACCCTTCGGTATGGCTTCCGGTGTTCCAGG
AGCGCCGGAAAGCACGCATTGCGTCGTCCAAATCAACAGGAAGGTG
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Protein Sequence |
>gi|17988372|ref|NP_541005.1| ATPase VIRB4-like protein [Brucella melitensis bv. 1 str. 16M] MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEKLLIRKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQKLAIRKLDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEIRTASATRYARGIDFKDYANHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHNAPMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQQVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQERRKARIASSKSTGR
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Molecule Role |
Virulence factor |
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Molecule Role Annotation |
MUTATION: A mutant strain of B abortus that contains an in-frame deletion in virB4 is unable to replicate in macrophages and survives in mice (Watarai et al., 2002). ntracellular replication was inhibited in wild-type B abortus after introducing a plasmid expressing a mutant VirB4 altered in the NTP -binding region. VirB4 containing the intact NTP -binding region is essential for evasion of fusion with lysosomes (11988518).
The ruffling associated with internalization of the virB4 mutant results in a more rapid uptake than for the wild-type strain. The virB4 mutant shows primarily small regions of phalloidin staining at the sites of binding. Macrophages incubated simultaneously with B abortus and the fluid-phase marker tetramethyl rhodamine isothiocyanate (TRITC)-dextran accumulate the marker in large vacuoles containing the wild-type strain, but little or no marker accumulates in phagosomes containing the virB4 mutant. Similarly, phase-contrast micrographs have shown the wild-type strain in large phase-transparent compartments, but the virB4 mutant is in much smaller compartments (14738898).
Intracellular growth-defective virB4 mutant and attenuated vaccine strain S19 did not induce abortion (Kim et al., 2005).
The B abortus virB4 mutant was completely cleared from the spleens of mice after 4 weeks, while the pncA mutant showed a 1.5-log reduction of the number of bacteria isolated from spleens after 10 weeks. Splenomegaly was not observed at all in mice infected with virB4 mutant (Kim et al., 2004). |
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COG
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COG3451U, under U: Intracellular trafficking, secretion, and vesicular transport |
| References |
Kim et al., 2004: Kim S, Kurokawa D, Watanabe K, Makino S, Shirahata T, Watarai M. Brucella abortus nicotinamidase (PncA) contributes to its intracellular replication and infectivity in mice. FEMS microbiology letters. 2004; 234(2); 289-295. [PubMed: 15135535].
Kim et al., 2005: Kim S, Lee DS, Watanabe K, Furuoka H, Suzuki H, Watarai M. Interferon-gamma promotes abortion due to Brucella infection in pregnant mice. BMC microbiology. 2005; 5; 22. [PubMed: 15869716].
Watarai et al., 2002: Watarai M, Makino S, Shirahata T. An essential virulence protein of Brucella abortus, VirB4, requires an intact nucleoside-triphosphate-binding domain. Microbiology (Reading, England). 2002; 148(Pt 5); 1439-1446. [PubMed: 11988518].
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