Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

virB2 from Brucella melitensis bv. 1 str. 16M

Victor ID 1329
Gene Name virB2 from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197797
NCBI Protein GI 17988370
Locus Tag BMEII0026
Protein Accession NP_541003.1
Other Database IDs UniProtKB-ID: D0B5S0_BRUME
UniRef100: UniRef100_Q2YIT6
UniRef90: UniRef90_Q2YIT6
UniRef50: UniRef50_Q2YIT6
UniParc: UPI0000058309
EMBL: GG703779
EMBL-CDS: EEW86962.1
RefSeq_NT: NC_003318.1
OMA: MAFAHAR
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 26013
Gene Ending Position 26330
Gene Strand (Orientation) +
Protein Name VirB2
DNA Sequence 
>gi|17988344:26013-26330 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
CATGAAAACCGCTTCCCCCAGCAAGAAGTCGCTGTCGCGGATTCTACCTCACCTGCTGCTGGCCCTCATT
GTCTCCATCGCTGCAATCGAGCCTAACCTGGCGCACGCCAACGGTGGCCTCGATAAGGTAAATACAAGCA
TGCAAAAAGTGCTGGACTTGCTAAGCGGCGTATCGATCACCATCGTTACCATAGCCATCATCTGGTCCGG
TTACAAGATGGCATTCCGGCACGCCCGCTTCATGGATGTAGTGCCGGTGCTGGGCGGCGCGCTGGTGGTT
GGCGCTGCCGCCGAAATTGCCTCTTACCTGCTTAGGTA
Protein Sequence 
>gi|17988370|ref|NP_541003.1| VirB2 [Brucella melitensis bv. 1 str. 16M] MKTASPSKKSLSRILPHLLLALIVSIAAIEPNLAHANGGLDKVNTSMQKVLDLLSGVSITIVTIAIIWSGYKMAFRHARFMDVVPVLGGALVVGAAAEIASYLLR
Molecule Role Virulence factor
Molecule Role Annotation MUTATION: The Brucella abortus virB operon, encoding a type IV secretion system (T4SS), is required for intracellular replication and persistent infection in the mouse model. The products of the first two genes of the virB operon, virB1 and virB2, are predicted to be localized at the bacterial surface. Both mutants were shown to be nonpolar, as demonstrated by their ability to express the downstream gene virB5 during stationary phase of growth in vitro. Both VirB1 and VirB2 were essential for intracellular replication in J774 macrophages. The nonpolar virB2 mutant was unable to cause persistent infection in the mouse model, demonstrating the essential role of VirB2 in the function of the T4SS apparatus during infection (den et al., 2004).

Polar mutations in the virB1 to virB2 intergenic region or in virB2 reduced the detection of VirB5 to a greater extent than they did that of VirB12. A virB1 mutation also eliminates the transcription of virB12 in B suis (Sun et al., 2005).
References
den et al., 2004: den Hartigh AB, Sun YH, Sondervan D, Heuvelmans N, Reinders MO, Ficht TA, Tsolis RM. Differential requirements for VirB1 and VirB2 during Brucella abortus infection. Infection and immunity. 2004; 72(9); 5143-5149. [PubMed: 15322008].
Sun et al., 2005: Sun YH, Rolán HG, den Hartigh AB, Sondervan D, Tsolis RM. Brucella abortus virB12 is expressed during infection but is not an essential component of the type IV secretion system. Infection and immunity. 2005; 73(9); 6048-6054. [PubMed: 16113325].