Search: for Help
About
Introduction
Statistics
Your PHIDIAS
Register or Login
Philert
Submission
Curated Data
Victors
BBP (Brucella)
Phinfo
Phinet
HazARD
Data Analysis
Phigen
Pacodom
BLAST
Help & Documents
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

hemH from Brucella melitensis bv. 1 str. 16M

Victor ID 1327
Gene Name hemH from Brucella melitensis bv. 1 str. 16M
Sequence Strain (Species/Organism) Brucella melitensis bv. 1 str. 16M
NCBI Gene ID 1197789
NCBI Protein GI 17988362
Locus Tag BMEII0018
Protein Accession NP_540995.1
Other Database IDs UniProtKB-ID: D0B5S8_BRUME
UniRef100: UniRef100_C0RK28
UniRef90: UniRef90_B2SCP3
UniRef50: UniRef50_A9MDJ3
UniParc: UPI0000058301
EMBL: GG703779
EMBL-CDS: EEW86970.1
RefSeq_NT: NC_003318.1
OMA: TIEEIGM
Taxonomy ID 224914
Chromosome No II
Gene Starting Position 16325
Gene Ending Position 17383
Gene Strand (Orientation) +
Protein Name ferrochelatase
DNA Sequence 
>gi|17988344:16325-17383 Brucella melitensis bv. 1 str. 16M chromosome chromosome II, complete sequence
TATGAGTGGAACGGACAAGGTGCGTGTGAACGTCAGCCAGACAGCGCAGACCCCGCTGCATACATCGGCC
AAACTGCCGAAAGTCGGGGTTTTGCTGGTCAATCTCGGCACGCCGGACGGCACGAGCTACGGCCCGATGC
GGCGCTATCTTGCCGAATTTCTCTCCGACCGCCGCGTCATCGAATGGTCGCGCCTTATCTGGTACCCGAT
CCTTTACGGTATTGTTCTCAACACACGGCCCAGGCGTTCCGGCAGGCTCTATGATCGCATCTGGAATCAC
GAGAATAATGAATCGCCCCTGCGCACCTATACGCGCGCGCAGGGCGAGAAACTTGCCAAAGCACTTAGCG
ACCAACCCAATGTCGTGGTGGACTGGGCGATGCGCTATGGCCAGCCTTCCATCGAGAGCATAACCGACCG
CCTGCTTCAGCAGGGCTGCGAGCGGATTGTGATATTCCCGCTCTATCCGCAATATTCCGCCACCACCACG
GCGACGGTGAACGACAAGTTCTTCGAGGCGCTGATGAAAAAGCGCTTCATGCCTGCAATCCGCACCGTGC
CGTCATACGAGGCCGAGCCGGTCTATATCGACGCGCTGGCGCGTTCCGTCGAAAAGCATCTGGCTACGCT
TTCCTTCAAGCCCGAAGTCATCCTCACCTCCTATCACGGCATTCCGAAAAGCTATTCGGACAAGGGCGAC
CCCTATCGCCAGCAATGCCTTGAAACGACGCGGCTTCTGCGTGAGCGCCTCGGCCTTGGCGAAGACGAGA
TGCGCGCCACCTTCCAGTCACGCTTCGGCCCGGAAGAATGGCTCCAGCCCTATACCGACGAAACCGTGAA
GGAACTGGCCAAAAACGGCGTCAAATCGGTGGCGGTGCTCAATCCTGGTTTCGTGGCCGATTGCCTGGAA
ACGGTGGACGAAATCGGCAATGAGGCAGCGGAAGAGTTTCTTGAAAACGGCGGCGAAAACTTCAGCCATA
TTCCCTGCCTCAACGATAGCGAAGAGGGCATGAAGGTCATTGAGACGCTGGTGCGCCGCGAATTGCTGGG
ATGGGTTTA
Protein Sequence 
>gi|17988362|ref|NP_540995.1| ferrochelatase [Brucella melitensis bv. 1 str. 16M] MSGTDKVRVNVSQTAQTPLHTSAKLPKVGVLLVNLGTPDGTSYGPMRRYLAEFLSDRRVIEWSRLIWYPILYGIVLNTRPRRSGRLYDRIWNHENNESPLRTYTRAQGEKLAKALSDQPNVVVDWAMRYGQPSIESITDRLLQQGCERIVIFPLYPQYSATTTATVNDKFFEALMKKRFMPAIRTVPSYEAEPVYIDALARSVEKHLATLSFKPEVILTSYHGIPKSYSDKGDPYRQQCLETTRLLRERLGLGEDEMRATFQSRFGPEEWLQPYTDETVKELAKNGVKSVAVLNPGFVADCLETVDEIGNEAAEEFLENGGENFSHIPCLNDSEEGMKVIETLVRRELLGWV
Molecule Role Virulence factor
Molecule Role Annotation FUNCTION: Catalyzes the ferrous insertion into protoporphyrin IX(UniProt: P0A3D7).

CATALYTIC ACTIVITY: Protoporphyrin + Fe(2+) = protoheme + 2 H(+)(UniProt: P0A3D7).

PATHWAY: Protoheme biosynthesis; last step(UniProt: P0A3D7).

SUBCELLULAR LOCATION: Cytoplasm (By similarity)(UniProt: P0A3D7).

SIMILARITY: Belongs to the ferrochelatase family(UniProt: P0A3D7).

MUTATION: A hemH knockout B. abortus mutant displayed auxotrophy for hemin, defective intracellular survival inside J774 and HeLa cells, and lack of virulence in BALBc mice. This phenotype was overcome by complementing the mutant strain with a plasmid harboring wild-type hemH. These data demonstrate that B abortus synthesizes its own heme and also has the ability to use an external source of heme (Almirón et al., 2001).
COG COG0276H, under H: Coenzyme transport and metabolism
References
Almirón et al., 2001: Almirón M, Martínez M, Sanjuan N, Ugalde RA. Ferrochelatase is present in Brucella abortus and is critical for its intracellular survival and virulence. Infection and immunity. 2001; 69(10); 6225-6230. [PubMed: 11553564].